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The map label for this gene is seqA [H]

Identifier: 157369483

GI number: 157369483

Start: 1364723

End: 1365262

Strand: Direct

Name: seqA [H]

Synonym: Spro_1240

Alternate gene names: 157369483

Gene position: 1364723-1365262 (Clockwise)

Preceding gene: 157369478

Following gene: 157369484

Centisome position: 25.05

GC content: 55.0

Gene sequence:

>540_bases
ATGAAAACTATTGAAGTCGACGAAGAGCTCTACCGTTATATTGCCAGCCACACGCAACATATTGGTGAGAGCGCGTCCGA
TATTTTGCGCCGCATGTTGAAATTTACCGCAGGTCAGCCGTTACGTGCGGTGCCGGCAGCCAGTGCCCAGAGCGCTGAAC
AGGATAAAATTGTTGCCGCGTCGCGCCCGCGCGATCGCGTACGTGCCGTGCGTGAGCTGCTGTTGTCGGATGAATATGCC
GAGCAGAACAAAGCGGTGAACCGTTTTATGCTGGTACTTTCCACCCTGTATACCCTCGACGCGGCCGGTTTTGCCACGGC
GACCGAGGCGTTGCATGGCCGTACCCGCACCTATTTTGCCGGCGATCAGCAAACCCTGCTGGCAAACGGAACGCACACCA
AACCAAAGCACGTTCCGGGCACCCCTTACTGGGTGATCACCAATACCAATACCGGTCGTAAACGCAGCATGGTGGAACAC
ATCATGCAGGCTATGCAGTTCCCAGCGGAACTGACAGAAAAAGTTTGCGGTACTATCTAA

Upstream 100 bases:

>100_bases
TTGTGATGTTCGCATCTGCGCTTTTAACCTTATAATCCCATGGCTTGAATGCAGGATCTGGCTTCAGGTTCTAAGTAGAA
AAAAACAGTACTGGATAAAG

Downstream 100 bases:

>100_bases
TTTAGCGAACAGCCCGTCATGGTGGCCGTGAGCGACATATTGCCAGGCCGCAGGACAGGGATAGGGAGATATGTCGATGG
CGAATAATCCACGTGCCGGG

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MKTIEVDEELYRYIASHTQHIGESASDILRRMLKFTAGQPLRAVPAASAQSAEQDKIVAASRPRDRVRAVRELLLSDEYA
EQNKAVNRFMLVLSTLYTLDAAGFATATEALHGRTRTYFAGDQQTLLANGTHTKPKHVPGTPYWVITNTNTGRKRSMVEH
IMQAMQFPAELTEKVCGTI

Sequences:

>Translated_179_residues
MKTIEVDEELYRYIASHTQHIGESASDILRRMLKFTAGQPLRAVPAASAQSAEQDKIVAASRPRDRVRAVRELLLSDEYA
EQNKAVNRFMLVLSTLYTLDAAGFATATEALHGRTRTYFAGDQQTLLANGTHTKPKHVPGTPYWVITNTNTGRKRSMVEH
IMQAMQFPAELTEKVCGTI
>Mature_179_residues
MKTIEVDEELYRYIASHTQHIGESASDILRRMLKFTAGQPLRAVPAASAQSAEQDKIVAASRPRDRVRAVRELLLSDEYA
EQNKAVNRFMLVLSTLYTLDAAGFATATEALHGRTRTYFAGDQQTLLANGTHTKPKHVPGTPYWVITNTNTGRKRSMVEH
IMQAMQFPAELTEKVCGTI

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form. Binds hemimethylated oriC. This process has

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=184, Percent_Identity=73.9130434782609, Blast_Score=275, Evalue=9e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005621
- InterPro:   IPR010985 [H]

Pfam domain/function: PF03925 SeqA [H]

EC number: NA

Molecular weight: Translated: 19977; Mature: 19977

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIEVDEELYRYIASHTQHIGESASDILRRMLKFTAGQPLRAVPAASAQSAEQDKIVAA
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
SRPRDRVRAVRELLLSDEYAEQNKAVNRFMLVLSTLYTLDAAGFATATEALHGRTRTYFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GDQQTLLANGTHTKPKHVPGTPYWVITNTNTGRKRSMVEHIMQAMQFPAELTEKVCGTI
CCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MKTIEVDEELYRYIASHTQHIGESASDILRRMLKFTAGQPLRAVPAASAQSAEQDKIVAA
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
SRPRDRVRAVRELLLSDEYAEQNKAVNRFMLVLSTLYTLDAAGFATATEALHGRTRTYFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GDQQTLLANGTHTKPKHVPGTPYWVITNTNTGRKRSMVEHIMQAMQFPAELTEKVCGTI
CCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]