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The map label for this gene is lpfB [H]

Identifier: 157369297

GI number: 157369297

Start: 1160811

End: 1161515

Strand: Reverse

Name: lpfB [H]

Synonym: Spro_1054

Alternate gene names: 157369297

Gene position: 1161515-1160811 (Counterclockwise)

Preceding gene: 157369298

Following gene: 157369296

Centisome position: 21.32

GC content: 64.4

Gene sequence:

>705_bases
ATGACCTATCGGCTCACTGCACGCTATCGCCGTATTCTGATGACCGCCGCGCTGATGGCCGGCCTGTTGACGACCAGCCT
GCCGACTCAGGCCGGCGTGGTGGTCGGCGCGACCCGCCTGGTCTACGAGGGTGACAAAAAAGAGAGTTCCCTGACCGTCA
GCAACCCGGACGCCTCGCCTTACCTTATCCAGTCCTGGGTCGAAACCGCCCCGAGCGACGAGAACAAAGCCCCCTTCATC
CTGACCCCGCCGCTGTTTCGCCTCGACGGGGGGCAACAAAACGTTCTGCGCGTAGTGCGGGTTGGCGGCGCGCTGGCCGA
AGACAGGGAAACGCTGTACTGGCTGAATGTCAAAGCCATCCCCTCGGCCGTGCGCCAGGACAACACCCTGCAAATCGCCA
TCAAGACGCGTATCAAACTGATTTTCCGCCCGAAGGCCCTGAGCGGCCGCCAGCCGGAGAGCGTCGGCGGTCAACTGACC
TGGCGCCGGGTGGCGGGCAAGCTGGAGGTCAGCAACCCCACGCCGTTTTACATGAACTTTCAGGGTATCAAGGTCAATGG
CCTGCCGGTGAAGGACGCCACCTACGTGGCCCCGTTCAGCAGTGCGACCCTGACCCTGCCGGGCGACGGCCGTGAGGTCA
GCTGGCGGCTGCTGAACGATTTCGGCGGCGCGGGGCAATGGCTGAAAGGCACCGTGGCCGGCTAG

Upstream 100 bases:

>100_bases
CGGGACGGCGAACGCCACCGCCAGCTTCACCATCAACTACAACTGATACCACTCCGGGGCGGCAGCGACTGCTGCCCCTT
TTCCTGCCGGAGACAATGCC

Downstream 100 bases:

>100_bases
CCGGTCGCGGCAACGCAAGCAAGGAGCATGCGATGCAGCAACAACGCACTACAGGAATAAAACCGCTGAGGATGATGATA
CTGACCGCACTGGCGTTGTC

Product: pili assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MTYRLTARYRRILMTAALMAGLLTTSLPTQAGVVVGATRLVYEGDKKESSLTVSNPDASPYLIQSWVETAPSDENKAPFI
LTPPLFRLDGGQQNVLRVVRVGGALAEDRETLYWLNVKAIPSAVRQDNTLQIAIKTRIKLIFRPKALSGRQPESVGGQLT
WRRVAGKLEVSNPTPFYMNFQGIKVNGLPVKDATYVAPFSSATLTLPGDGREVSWRLLNDFGGAGQWLKGTVAG

Sequences:

>Translated_234_residues
MTYRLTARYRRILMTAALMAGLLTTSLPTQAGVVVGATRLVYEGDKKESSLTVSNPDASPYLIQSWVETAPSDENKAPFI
LTPPLFRLDGGQQNVLRVVRVGGALAEDRETLYWLNVKAIPSAVRQDNTLQIAIKTRIKLIFRPKALSGRQPESVGGQLT
WRRVAGKLEVSNPTPFYMNFQGIKVNGLPVKDATYVAPFSSATLTLPGDGREVSWRLLNDFGGAGQWLKGTVAG
>Mature_233_residues
TYRLTARYRRILMTAALMAGLLTTSLPTQAGVVVGATRLVYEGDKKESSLTVSNPDASPYLIQSWVETAPSDENKAPFIL
TPPLFRLDGGQQNVLRVVRVGGALAEDRETLYWLNVKAIPSAVRQDNTLQIAIKTRIKLIFRPKALSGRQPESVGGQLTW
RRVAGKLEVSNPTPFYMNFQGIKVNGLPVKDATYVAPFSSATLTLPGDGREVSWRLLNDFGGAGQWLKGTVAG

Specific function: Required for the biogenesis of long polar fimbria; binds and interact with lpfA [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1790771, Length=230, Percent_Identity=39.1304347826087, Blast_Score=151, Evalue=3e-38,
Organism=Escherichia coli, GI1789532, Length=220, Percent_Identity=37.7272727272727, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787171, Length=223, Percent_Identity=39.4618834080717, Blast_Score=143, Evalue=8e-36,
Organism=Escherichia coli, GI1786743, Length=217, Percent_Identity=32.7188940092166, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786333, Length=234, Percent_Identity=31.6239316239316, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1786936, Length=212, Percent_Identity=34.4339622641509, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788677, Length=194, Percent_Identity=33.5051546391753, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082203, Length=228, Percent_Identity=28.0701754385965, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081806, Length=223, Percent_Identity=31.390134529148, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788428, Length=226, Percent_Identity=26.1061946902655, Blast_Score=87, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 25562; Mature: 25431

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYRLTARYRRILMTAALMAGLLTTSLPTQAGVVVGATRLVYEGDKKESSLTVSNPDASP
CEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCEEEEECCCCCH
YLIQSWVETAPSDENKAPFILTPPLFRLDGGQQNVLRVVRVGGALAEDRETLYWLNVKAI
HHHHHHHHHCCCCCCCCCEEECCCCEEECCCHHHHHHHHHHCCCCCCCCCEEEEEEEEHH
PSAVRQDNTLQIAIKTRIKLIFRPKALSGRQPESVGGQLTWRRVAGKLEVSNPTPFYMNF
HHHHCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEECCCCCEEEEE
QGIKVNGLPVKDATYVAPFSSATLTLPGDGREVSWRLLNDFGGAGQWLKGTVAG
EEEEECCCCCCCCEEEECCCCCEEEECCCCCEEEEEEEECCCCCCCEEEEECCC
>Mature Secondary Structure 
TYRLTARYRRILMTAALMAGLLTTSLPTQAGVVVGATRLVYEGDKKESSLTVSNPDASP
EEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCEEEEECCCCCH
YLIQSWVETAPSDENKAPFILTPPLFRLDGGQQNVLRVVRVGGALAEDRETLYWLNVKAI
HHHHHHHHHCCCCCCCCCEEECCCCEEECCCHHHHHHHHHHCCCCCCCCCEEEEEEEEHH
PSAVRQDNTLQIAIKTRIKLIFRPKALSGRQPESVGGQLTWRRVAGKLEVSNPTPFYMNF
HHHHCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEECCCCCEEEEE
QGIKVNGLPVKDATYVAPFSSATLTLPGDGREVSWRLLNDFGGAGQWLKGTVAG
EEEEECCCCCCCCEEEECCCCCEEEECCCCCEEEEEEEECCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7721701; 11677609 [H]