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The map label for this gene is dsbA [H]

Identifier: 157369226

GI number: 157369226

Start: 1087251

End: 1087892

Strand: Direct

Name: dsbA [H]

Synonym: Spro_0983

Alternate gene names: 157369226

Gene position: 1087251-1087892 (Clockwise)

Preceding gene: 157369223

Following gene: 157369227

Centisome position: 19.95

GC content: 56.39

Gene sequence:

>642_bases
ATGTTGGCTAAAGTTAATCGTCTGCTCGCCGGTGCGTTACTGGTGTTATTGCTGCCTGCCGTTGCTGCGGAGGCGGATTA
CCGCGCGGGCGAGCAATATACTCGCCTGGATAAGCCGGTGGCGTCGGCCCCTGCGGTGGTGGAGTTTTTCTCTTTCTATT
GCGGCCCGTGCTATCAGTTTGCCGAGACTTACCATGTGGGCAGCACCGTCAGCCAGGCATTGCCTGCGGGCACCAAACTG
ACTAAATACCACGTTGGCCTGATGGGCAAACTGGGGAATGAGCTGACCGAGGCCTGGTCGGTGGCGATGGTGATGGGCAT
TGAGGACAAAATCGAAGGCATGCTGTTCGACGAGCTGCAAAAGAAGCGTGCCATCAACAATGAACAGGATATTCAGCGGG
TGTTTGCCGCTGCCGGTGTTGATGCGGCCACCTATGAAAATGCGCGTCACAGCCTGCTGGTGAAGGGCATGATCGCCAAA
CAGAATGAAGCGGTCAAAGCGCTGGACGTGCGCGCCACGCCATCGTTTTATGTTTCCGGCAAGTACAAGATCGACAATGC
CGGAATGGCGAGCCAAAGCGTTGACGGCTATGCCAAAGAGTACGCCGCCGTAGTCCACTATCTGCTGACCACCCAGCCTT
AA

Upstream 100 bases:

>100_bases
TCCCACATAAACATAAGGTAATAAAAACTGGTCTTGTCCCGATAATTTGATTATAAACACCGGCGTTAGGGACAATGTTC
TCATCTGAATTGGAGTAGTT

Downstream 100 bases:

>100_bases
GATTTTCCCCTTACTCTTGTTTTTACCTCCCCGGCTACGGCCGGGTTTTTCATATCGGGTGGCGCAAGACGCAATCTGTG
ATCCCAGCGCCAGATCCCGG

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MLAKVNRLLAGALLVLLLPAVAAEADYRAGEQYTRLDKPVASAPAVVEFFSFYCGPCYQFAETYHVGSTVSQALPAGTKL
TKYHVGLMGKLGNELTEAWSVAMVMGIEDKIEGMLFDELQKKRAINNEQDIQRVFAAAGVDAATYENARHSLLVKGMIAK
QNEAVKALDVRATPSFYVSGKYKIDNAGMASQSVDGYAKEYAAVVHYLLTTQP

Sequences:

>Translated_213_residues
MLAKVNRLLAGALLVLLLPAVAAEADYRAGEQYTRLDKPVASAPAVVEFFSFYCGPCYQFAETYHVGSTVSQALPAGTKL
TKYHVGLMGKLGNELTEAWSVAMVMGIEDKIEGMLFDELQKKRAINNEQDIQRVFAAAGVDAATYENARHSLLVKGMIAK
QNEAVKALDVRATPSFYVSGKYKIDNAGMASQSVDGYAKEYAAVVHYLLTTQP
>Mature_213_residues
MLAKVNRLLAGALLVLLLPAVAAEADYRAGEQYTRLDKPVASAPAVVEFFSFYCGPCYQFAETYHVGSTVSQALPAGTKL
TKYHVGLMGKLGNELTEAWSVAMVMGIEDKIEGMLFDELQKKRAINNEQDIQRVFAAAGVDAATYENARHSLLVKGMIAK
QNEAVKALDVRATPSFYVSGKYKIDNAGMASQSVDGYAKEYAAVVHYLLTTQP

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=198, Percent_Identity=44.9494949494949, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23150; Mature: 23150

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKVNRLLAGALLVLLLPAVAAEADYRAGEQYTRLDKPVASAPAVVEFFSFYCGPCYQF
CCHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHCCCCCCCHHHHHHHHHHCCHHHHH
AETYHVGSTVSQALPAGTKLTKYHVGLMGKLGNELTEAWSVAMVMGIEDKIEGMLFDELQ
HHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KKRAINNEQDIQRVFAAAGVDAATYENARHSLLVKGMIAKQNEAVKALDVRATPSFYVSG
HHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCEEEEC
KYKIDNAGMASQSVDGYAKEYAAVVHYLLTTQP
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAKVNRLLAGALLVLLLPAVAAEADYRAGEQYTRLDKPVASAPAVVEFFSFYCGPCYQF
CCHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHCCCCCCCHHHHHHHHHHCCHHHHH
AETYHVGSTVSQALPAGTKLTKYHVGLMGKLGNELTEAWSVAMVMGIEDKIEGMLFDELQ
HHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KKRAINNEQDIQRVFAAAGVDAATYENARHSLLVKGMIAKQNEAVKALDVRATPSFYVSG
HHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCEEEEC
KYKIDNAGMASQSVDGYAKEYAAVVHYLLTTQP
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7476168 [H]