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The map label for this gene is tcyB [H]

Identifier: 157369178

GI number: 157369178

Start: 1033052

End: 1033717

Strand: Direct

Name: tcyB [H]

Synonym: Spro_0933

Alternate gene names: 157369178

Gene position: 1033052-1033717 (Clockwise)

Preceding gene: 157369177

Following gene: 157369179

Centisome position: 18.96

GC content: 60.66

Gene sequence:

>666_bases
ATGACCTATCAGCTGAACTTTCCCGCCCTGTGGCCTTACCTGCCCGAGTTGCTGGCCGGTTTGTTGACCACCATTGAGCT
GACGGCTCTGGCCACGTTAATCGGGGTGGCACTGGGGATTTGCGGTGCGGCGATCCGCAGCGGCAAGGCTAACCTGGTTA
GTCGCGTTTGGGGCATTTACGTTGAGGTGGTACGCAACACGCCGTTCGTGGTGCAACTGTTCTTCATCGTCTTCGGCCTG
CCGAGCCTGGGCTGGAAACTGACCGCCGGGCAGGCGGCGCTGCTGGCGATGGTGATTAACCTGGGGGCCTACAGTACCGA
GATTATCCGTGCCGGCATTCAGGTGACGCCGAAAGGCCAGTGGGAGGCCGGCAGGGTGCTGGGGCTCAGCCGCAGCCAGA
CGTTTTTGCGCATCGTGCTGCCACCGTCGCTGCAGCGGATTTATCCGGCGTTGGTCAGCCAGTGCATTATCGTCATGCTG
GGGTCATCGGTGGTGTCGCAGGTGTCGTTCGAAGAGTTGACCTTCGCCGCCAACCTGATCCAGTCGCGCACCTTCCTCAG
TTTTGAGGTGTATCTGGTGACGGCGCTGTTTTACCTGTTGTTATCGATACTGATGCGCCAGCTGTTGCTGGCCGCCGGAC
GCCGTTTTCTGGGGAGCCAGGGCTGA

Upstream 100 bases:

>100_bases
GATTGAACAGGCATTGAAGGATAACACCCTTAATGGCCTGTCCGAGAAATGGCTCAAAGCGCCGCTGCCTGCCGACTTCG
GCGCATAACGGGGGCGCCTG

Downstream 100 bases:

>100_bases
TGCTGACTTTTACCGACTGGGACATTGTGCGCAACCTGCTGTTGGCGGCACGCTGGACCTTACTGCTGTCGCTGACGGCA
TTTTTGGGCGGCACCCTGGT

Product: polar amino acid ABC transporter inner membrane subunit

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTYQLNFPALWPYLPELLAGLLTTIELTALATLIGVALGICGAAIRSGKANLVSRVWGIYVEVVRNTPFVVQLFFIVFGL
PSLGWKLTAGQAALLAMVINLGAYSTEIIRAGIQVTPKGQWEAGRVLGLSRSQTFLRIVLPPSLQRIYPALVSQCIIVML
GSSVVSQVSFEELTFAANLIQSRTFLSFEVYLVTALFYLLLSILMRQLLLAAGRRFLGSQG

Sequences:

>Translated_221_residues
MTYQLNFPALWPYLPELLAGLLTTIELTALATLIGVALGICGAAIRSGKANLVSRVWGIYVEVVRNTPFVVQLFFIVFGL
PSLGWKLTAGQAALLAMVINLGAYSTEIIRAGIQVTPKGQWEAGRVLGLSRSQTFLRIVLPPSLQRIYPALVSQCIIVML
GSSVVSQVSFEELTFAANLIQSRTFLSFEVYLVTALFYLLLSILMRQLLLAAGRRFLGSQG
>Mature_220_residues
TYQLNFPALWPYLPELLAGLLTTIELTALATLIGVALGICGAAIRSGKANLVSRVWGIYVEVVRNTPFVVQLFFIVFGLP
SLGWKLTAGQAALLAMVINLGAYSTEIIRAGIQVTPKGQWEAGRVLGLSRSQTFLRIVLPPSLQRIYPALVSQCIIVMLG
SSVVSQVSFEELTFAANLIQSRTFLSFEVYLVTALFYLLLSILMRQLLLAAGRRFLGSQG

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787030, Length=214, Percent_Identity=32.7102803738318, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788226, Length=201, Percent_Identity=32.3383084577114, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1787087, Length=210, Percent_Identity=27.1428571428571, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787086, Length=213, Percent_Identity=28.6384976525822, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1786873, Length=224, Percent_Identity=29.9107142857143, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788646, Length=218, Percent_Identity=25.6880733944954, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI48994930, Length=215, Percent_Identity=28.8372093023256, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788645, Length=212, Percent_Identity=25.4716981132075, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786874, Length=219, Percent_Identity=25.1141552511416, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 24110; Mature: 23979

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYQLNFPALWPYLPELLAGLLTTIELTALATLIGVALGICGAAIRSGKANLVSRVWGIY
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VEVVRNTPFVVQLFFIVFGLPSLGWKLTAGQAALLAMVINLGAYSTEIIRAGIQVTPKGQ
HHHHHCCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHCCHHHHHHHCCCEECCCCC
WEAGRVLGLSRSQTFLRIVLPPSLQRIYPALVSQCIIVMLGSSVVSQVSFEELTFAANLI
CCCCCEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSRTFLSFEVYLVTALFYLLLSILMRQLLLAAGRRFLGSQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TYQLNFPALWPYLPELLAGLLTTIELTALATLIGVALGICGAAIRSGKANLVSRVWGIY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VEVVRNTPFVVQLFFIVFGLPSLGWKLTAGQAALLAMVINLGAYSTEIIRAGIQVTPKGQ
HHHHHCCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHCCHHHHHHHCCCEECCCCC
WEAGRVLGLSRSQTFLRIVLPPSLQRIYPALVSQCIIVMLGSSVVSQVSFEELTFAANLI
CCCCCEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSRTFLSFEVYLVTALFYLLLSILMRQLLLAAGRRFLGSQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]