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The map label for this gene is fliY [H]

Identifier: 157369177

GI number: 157369177

Start: 1032248

End: 1033039

Strand: Direct

Name: fliY [H]

Synonym: Spro_0932

Alternate gene names: 157369177

Gene position: 1032248-1033039 (Clockwise)

Preceding gene: 157369176

Following gene: 157369178

Centisome position: 18.94

GC content: 57.2

Gene sequence:

>792_bases
ATGAACTTCAAGAAAATCGGCGTAGTGGCATTAGGTGCAGTGCTGTTGATGGCTCAGGCCGCTTCGGCGATGGCGGATCA
ACTGCAGGAAATCCAGCAGCGCGGCGTGTTGCGGGTAGCGGTACCCCAGGACTTCCCGCCGTTCGGCTCCGTGGGCACCG
ATCTGCAGCCACAGGGTTATGACATCGATATGGCGCAGTATCTGGCCGACAAGATGAAACTTAAGTTGCAGCTGGTGCCG
GTCACCAGCGCCAACCGCGTGCCTTATCTGCAGACCAACAAGGTTGATTTGGTGATTTCCAGCCTGGGCAAAAATGCGGA
ACGCGAGAAGGCAATCGCCTTCAGCCGCACCTATGCGCCGTTCTTCCTGGGCGTATTTGGTCCGAAAGAATCGGTGTTGG
CGGACGCCACGCAGCTGAATGGTAAAACCATCGGCGTGACGCGCGGTGCGGTGGAAGACATGGTGCTGAGCGACACCGCG
CCAAAGGGCACGCAGATCAAGCGCTATGAAGACAATAACACCACGCTGTCGGCCTATCTGTCCGGTCAGGTGCAATACAT
CGCTACCGGCAATCTGGTGGTGAGCGCCATAGCACGGCAGAACCCGAGCAAGGCACCGGTGGCCAAATTTATGCTGAAAG
ATTCACCCTGCTATGTGGGGCTGCGTCTGGGTGAACCGGCGTTGCAAGCGAAAGTGGACCAACTGATTGAACAGGCATTG
AAGGATAACACCCTTAATGGCCTGTCCGAGAAATGGCTCAAAGCGCCGCTGCCTGCCGACTTCGGCGCATAA

Upstream 100 bases:

>100_bases
ACTAAAGCCGAACGTTCCCGACGTCGCTTATTGGCACATTAGTTGCAGAGTGATGATCAAAGAATCTTTTGTTTCATCAT
TTCACCATCGGGGATAGTCT

Downstream 100 bases:

>100_bases
CGGGGGCGCCTGATGACCTATCAGCTGAACTTTCCCGCCCTGTGGCCTTACCTGCCCGAGTTGCTGGCCGGTTTGTTGAC
CACCATTGAGCTGACGGCTC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MNFKKIGVVALGAVLLMAQAASAMADQLQEIQQRGVLRVAVPQDFPPFGSVGTDLQPQGYDIDMAQYLADKMKLKLQLVP
VTSANRVPYLQTNKVDLVISSLGKNAEREKAIAFSRTYAPFFLGVFGPKESVLADATQLNGKTIGVTRGAVEDMVLSDTA
PKGTQIKRYEDNNTTLSAYLSGQVQYIATGNLVVSAIARQNPSKAPVAKFMLKDSPCYVGLRLGEPALQAKVDQLIEQAL
KDNTLNGLSEKWLKAPLPADFGA

Sequences:

>Translated_263_residues
MNFKKIGVVALGAVLLMAQAASAMADQLQEIQQRGVLRVAVPQDFPPFGSVGTDLQPQGYDIDMAQYLADKMKLKLQLVP
VTSANRVPYLQTNKVDLVISSLGKNAEREKAIAFSRTYAPFFLGVFGPKESVLADATQLNGKTIGVTRGAVEDMVLSDTA
PKGTQIKRYEDNNTTLSAYLSGQVQYIATGNLVVSAIARQNPSKAPVAKFMLKDSPCYVGLRLGEPALQAKVDQLIEQAL
KDNTLNGLSEKWLKAPLPADFGA
>Mature_263_residues
MNFKKIGVVALGAVLLMAQAASAMADQLQEIQQRGVLRVAVPQDFPPFGSVGTDLQPQGYDIDMAQYLADKMKLKLQLVP
VTSANRVPYLQTNKVDLVISSLGKNAEREKAIAFSRTYAPFFLGVFGPKESVLADATQLNGKTIGVTRGAVEDMVLSDTA
PKGTQIKRYEDNNTTLSAYLSGQVQYIATGNLVVSAIARQNPSKAPVAKFMLKDSPCYVGLRLGEPALQAKVDQLIEQAL
KDNTLNGLSEKWLKAPLPADFGA

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=254, Percent_Identity=26.7716535433071, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787088, Length=226, Percent_Identity=26.9911504424779, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787085, Length=223, Percent_Identity=25.5605381165919, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786876, Length=243, Percent_Identity=24.2798353909465, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788648, Length=230, Percent_Identity=23.4782608695652, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788649, Length=258, Percent_Identity=23.6434108527132, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28359; Mature: 28359

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKKIGVVALGAVLLMAQAASAMADQLQEIQQRGVLRVAVPQDFPPFGSVGTDLQPQGY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCC
DIDMAQYLADKMKLKLQLVPVTSANRVPYLQTNKVDLVISSLGKNAEREKAIAFSRTYAP
CCHHHHHHHHHHEEEEEEEEECCCCCCCEEECCCHHHHHHHHCCCCCHHHHHHHHCCCCH
FFLGVFGPKESVLADATQLNGKTIGVTRGAVEDMVLSDTAPKGTQIKRYEDNNTTLSAYL
HEEEECCCCHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCEEEEEECCCCEEEEEE
SGQVQYIATGNLVVSAIARQNPSKAPVAKFMLKDSPCYVGLRLGEPALQAKVDQLIEQAL
CCEEEEEEECHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHH
KDNTLNGLSEKWLKAPLPADFGA
HCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNFKKIGVVALGAVLLMAQAASAMADQLQEIQQRGVLRVAVPQDFPPFGSVGTDLQPQGY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCC
DIDMAQYLADKMKLKLQLVPVTSANRVPYLQTNKVDLVISSLGKNAEREKAIAFSRTYAP
CCHHHHHHHHHHEEEEEEEEECCCCCCCEEECCCHHHHHHHHCCCCCHHHHHHHHCCCCH
FFLGVFGPKESVLADATQLNGKTIGVTRGAVEDMVLSDTAPKGTQIKRYEDNNTTLSAYL
HEEEECCCCHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCEEEEEECCCCEEEEEE
SGQVQYIATGNLVVSAIARQNPSKAPVAKFMLKDSPCYVGLRLGEPALQAKVDQLIEQAL
CCEEEEEEECHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHH
KDNTLNGLSEKWLKAPLPADFGA
HCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]