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The map label for this gene is gatA [H]

Identifier: 157369173

GI number: 157369173

Start: 1027844

End: 1029244

Strand: Reverse

Name: gatA [H]

Synonym: Spro_0928

Alternate gene names: 157369173

Gene position: 1029244-1027844 (Counterclockwise)

Preceding gene: 157369174

Following gene: 157369172

Centisome position: 18.89

GC content: 63.03

Gene sequence:

>1401_bases
ATGAATCCAATCAGCACACTGTCGATCGCCGAGATCCGCGCCGCCCTGCAGCAAGGTGCGATTTCCGCCCGTGAGGTAGC
CCAGCAAACGCTGGATAATATCGACGCCCAAAACCCTTCGCTTAACGCCTATACCCACGTCACCCGTGAACGTATGCTGA
CGGAGGCCGACAATCTCGATCGTGCCCGTGCCAAAGGGCAAGCGCTACCCAGCCTGGCGGCCATTCCTTATGCGGTGAAA
AATCTGTTCGATGTCACCGGGGAAACCACCCTGGCCGGTGCCAGCCTGTTTTGCGATAACCCACCCGCCAAACAAGACGC
CTGGGTGGTCAACCGGCTGGCGGCACAGGGCGCGCTGCTCTCCGGCATGCTGAATATGGACGCCTATGCCTACGGCTTCA
CCACCGAAAACAGCCACTACGGCGCGACGCGTAACCCACACGACACCACGCGTATTGCCGGGGGGTCCTCTGGGGGTTCA
GCGGCTGCGGTCGCCGCCGGGCTGGTAACATTTTCACTCGGCAGCGATACCAACGGCTCAATACGCGTGCCCTCTTCGCT
GTGCGGCATCTTCGGGCTGAAGCCGACCTTTGGCCGCCTGTCGCGCAGCGGCAGCCAGCCGTTTGTCGCCAGTCTGGATC
ATGTCGGCCCCTTTGCCCGGCGGGTGCGCGATCTGGCCGCGGTGTATGACGTGATGCAGGGCACCGATATCGAGGACCGC
TTCCAGGCGGAAAAACCGATCACCCAAACCGAGTCGCTGCTGCCGCGTGGCCAACAAGGGTTACGTTGCGCGGTGCTGGC
CGGCTATTTCCAGCAATGGTGCAATGAAGACGCCAAAGCGGCGGTGCAGCAGGTCGCTCAGGCGCTGGAGGCCGTCGCCG
AAGTCGAACTGCCCCAGGCTGAACTGGCGCGTTCGGCGGCGTTTATTCTCACCGCCGCCGAGGGAGGTAACCAGTATCTG
CCGCAGTTGCGCAGCCAACCCGAGCGCTTTGAACCGCTGTCGCGCGAGCGGCTGCTTGCCGGGGCCATGCTGCCCGCCGC
CTGGTACCTGCAGGCACAACGTTTCCGCACCCATTTCCAGCAGCAGGTATTGCCGCTGTTTGAACACTGGGACATTCTGA
TCGCCCCGGCCACGCCGTGCGCCGCGACGGTCATTGGTCAGGAAACCCTACGTATCAACGGCACCGACCTGCCGATCCGC
GCCGGCATGGGTATGCTGACACAGCCGATCTCCTTTCTCGGCCTGCCGGTGGTGACGGTGCCGCTGAAAACCGCGGCGGG
GCTACCGATCGGGCTGCAGCTTATCGCACCGCCATGGCGTGAAGATCTGGCACTGCGAGCCGCCTATGCGCTGGAACAAC
GCGGCGTAGCTACCTGTGTTTTACCAGGGAAACAGGATTAA

Upstream 100 bases:

>100_bases
AGGATCTGCTGATCCAATTCAACCGCATCGCCGCAATGGCAGAGCCGCTGATGGCCTTGCCGCTCGATCAGCGTCTGGAA
ATTGCCGGGGTATATCGAGC

Downstream 100 bases:

>100_bases
CTAATGAAGAGTGAATATATCGATCGACCGGCAGTGCTGGCAGAAGTGAACGCCGCCTTTTACCGTTACGAACAGGCGCT
GACTGGCAATGATATTGACG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MNPISTLSIAEIRAALQQGAISAREVAQQTLDNIDAQNPSLNAYTHVTRERMLTEADNLDRARAKGQALPSLAAIPYAVK
NLFDVTGETTLAGASLFCDNPPAKQDAWVVNRLAAQGALLSGMLNMDAYAYGFTTENSHYGATRNPHDTTRIAGGSSGGS
AAAVAAGLVTFSLGSDTNGSIRVPSSLCGIFGLKPTFGRLSRSGSQPFVASLDHVGPFARRVRDLAAVYDVMQGTDIEDR
FQAEKPITQTESLLPRGQQGLRCAVLAGYFQQWCNEDAKAAVQQVAQALEAVAEVELPQAELARSAAFILTAAEGGNQYL
PQLRSQPERFEPLSRERLLAGAMLPAAWYLQAQRFRTHFQQQVLPLFEHWDILIAPATPCAATVIGQETLRINGTDLPIR
AGMGMLTQPISFLGLPVVTVPLKTAAGLPIGLQLIAPPWREDLALRAAYALEQRGVATCVLPGKQD

Sequences:

>Translated_466_residues
MNPISTLSIAEIRAALQQGAISAREVAQQTLDNIDAQNPSLNAYTHVTRERMLTEADNLDRARAKGQALPSLAAIPYAVK
NLFDVTGETTLAGASLFCDNPPAKQDAWVVNRLAAQGALLSGMLNMDAYAYGFTTENSHYGATRNPHDTTRIAGGSSGGS
AAAVAAGLVTFSLGSDTNGSIRVPSSLCGIFGLKPTFGRLSRSGSQPFVASLDHVGPFARRVRDLAAVYDVMQGTDIEDR
FQAEKPITQTESLLPRGQQGLRCAVLAGYFQQWCNEDAKAAVQQVAQALEAVAEVELPQAELARSAAFILTAAEGGNQYL
PQLRSQPERFEPLSRERLLAGAMLPAAWYLQAQRFRTHFQQQVLPLFEHWDILIAPATPCAATVIGQETLRINGTDLPIR
AGMGMLTQPISFLGLPVVTVPLKTAAGLPIGLQLIAPPWREDLALRAAYALEQRGVATCVLPGKQD
>Mature_466_residues
MNPISTLSIAEIRAALQQGAISAREVAQQTLDNIDAQNPSLNAYTHVTRERMLTEADNLDRARAKGQALPSLAAIPYAVK
NLFDVTGETTLAGASLFCDNPPAKQDAWVVNRLAAQGALLSGMLNMDAYAYGFTTENSHYGATRNPHDTTRIAGGSSGGS
AAAVAAGLVTFSLGSDTNGSIRVPSSLCGIFGLKPTFGRLSRSGSQPFVASLDHVGPFARRVRDLAAVYDVMQGTDIEDR
FQAEKPITQTESLLPRGQQGLRCAVLAGYFQQWCNEDAKAAVQQVAQALEAVAEVELPQAELARSAAFILTAAEGGNQYL
PQLRSQPERFEPLSRERLLAGAMLPAAWYLQAQRFRTHFQQQVLPLFEHWDILIAPATPCAATVIGQETLRINGTDLPIR
AGMGMLTQPISFLGLPVVTVPLKTAAGLPIGLQLIAPPWREDLALRAAYALEQRGVATCVLPGKQD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=29.0581162324649, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI195972892, Length=248, Percent_Identity=31.0483870967742, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI166795287, Length=155, Percent_Identity=39.3548387096774, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=438, Percent_Identity=28.9954337899543, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17556264, Length=269, Percent_Identity=32.7137546468402, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=482, Percent_Identity=24.4813278008299, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=311, Percent_Identity=26.6881028938907, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=510, Percent_Identity=23.921568627451, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=206, Percent_Identity=32.5242718446602, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=200, Percent_Identity=33.5, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=227, Percent_Identity=27.3127753303965, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=416, Percent_Identity=29.3269230769231, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323950, Length=409, Percent_Identity=25.4278728606357, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320448, Length=232, Percent_Identity=29.7413793103448, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=31.8367346938775, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24644968, Length=500, Percent_Identity=26.8, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI161078093, Length=492, Percent_Identity=27.6422764227642, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24652985, Length=479, Percent_Identity=25.678496868476, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19922090, Length=479, Percent_Identity=25.678496868476, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24652981, Length=479, Percent_Identity=25.678496868476, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24652983, Length=479, Percent_Identity=25.678496868476, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24648435, Length=470, Percent_Identity=25.9574468085106, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24648437, Length=470, Percent_Identity=25.9574468085106, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI45550774, Length=470, Percent_Identity=25.9574468085106, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21356731, Length=483, Percent_Identity=23.8095238095238, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648441, Length=417, Percent_Identity=25.8992805755396, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648439, Length=417, Percent_Identity=25.8992805755396, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 49942; Mature: 49942

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPISTLSIAEIRAALQQGAISAREVAQQTLDNIDAQNPSLNAYTHVTRERMLTEADNLD
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHH
RARAKGQALPSLAAIPYAVKNLFDVTGETTLAGASLFCDNPPAKQDAWVVNRLAAQGALL
HHHHHCCCCCHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCHHHHHHHHHHHHHHH
SGMLNMDAYAYGFTTENSHYGATRNPHDTTRIAGGSSGGSAAAVAAGLVTFSLGSDTNGS
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHEEECCCCCCCC
IRVPSSLCGIFGLKPTFGRLSRSGSQPFVASLDHVGPFARRVRDLAAVYDVMQGTDIEDR
EECCHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHH
FQAEKPITQTESLLPRGQQGLRCAVLAGYFQQWCNEDAKAAVQQVAQALEAVAEVELPQA
HHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHH
ELARSAAFILTAAEGGNQYLPQLRSQPERFEPLSRERLLAGAMLPAAWYLQAQRFRTHFQ
HHHCCEEEEEEECCCCCHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQVLPLFEHWDILIAPATPCAATVIGQETLRINGTDLPIRAGMGMLTQPISFLGLPVVTV
HHHHHHHHCCCEEEECCCCHHHHHHCCCEEEECCCCCCHHHCCCHHHHHHHHHCCCCEEE
PLKTAAGLPIGLQLIAPPWREDLALRAAYALEQRGVATCVLPGKQD
CHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCC
>Mature Secondary Structure
MNPISTLSIAEIRAALQQGAISAREVAQQTLDNIDAQNPSLNAYTHVTRERMLTEADNLD
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHH
RARAKGQALPSLAAIPYAVKNLFDVTGETTLAGASLFCDNPPAKQDAWVVNRLAAQGALL
HHHHHCCCCCHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCHHHHHHHHHHHHHHH
SGMLNMDAYAYGFTTENSHYGATRNPHDTTRIAGGSSGGSAAAVAAGLVTFSLGSDTNGS
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHEEECCCCCCCC
IRVPSSLCGIFGLKPTFGRLSRSGSQPFVASLDHVGPFARRVRDLAAVYDVMQGTDIEDR
EECCHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHH
FQAEKPITQTESLLPRGQQGLRCAVLAGYFQQWCNEDAKAAVQQVAQALEAVAEVELPQA
HHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHH
ELARSAAFILTAAEGGNQYLPQLRSQPERFEPLSRERLLAGAMLPAAWYLQAQRFRTHFQ
HHHCCEEEEEEECCCCCHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQVLPLFEHWDILIAPATPCAATVIGQETLRINGTDLPIRAGMGMLTQPISFLGLPVVTV
HHHHHHHHCCCEEEECCCCHHHHHHCCCEEEECCCCCCHHHCCCHHHHHHHHHCCCCEEE
PLKTAAGLPIGLQLIAPPWREDLALRAAYALEQRGVATCVLPGKQD
CHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA