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The map label for this gene is gshA

Identifier: 157369092

GI number: 157369092

Start: 943951

End: 945513

Strand: Direct

Name: gshA

Synonym: Spro_0847

Alternate gene names: 157369092

Gene position: 943951-945513 (Clockwise)

Preceding gene: 157369091

Following gene: 157369093

Centisome position: 17.32

GC content: 54.25

Gene sequence:

>1563_bases
TTGATCCCGGACGTATCCCAGGCGCTTTCTTGGTTGGAAGCCCATCCCCATGCATTAAAAGGCATTCGTCGCGGCATTGA
GCGTGAAACGCTGCGTGTCACCGAAGACGGAAAGCTGGCAACAACCGGACATCCGGAAAAATTGGGGGCGGCATTAACGC
ACCACTGGATAACCACAGACTTTGCCGAGGCGCTGTTAGAATTTATTACGCCGGTTGACGACAACCTCGATCACCTGCTG
ACGTTCTTGCGTGATATTCATCGTCACGTGGCGCGTAATCTGGGTGAGGAGCGCATGTGGCCGCTCAGCATGCCGTGCTT
TATTGAAGCCGAGCAGGACATTGAACTGGCGCAGTTCGGCTCGTCGAATATTGGCCGCATGAAGACGTTATACCGTGAAG
GGTTGAAGAACCGTTACGGCGCGCTGATGCAGACCATTTCCGGCGTGCACTACAACTTCTCCCTGCCGCTGGAGTTCTGG
CAGGCATGGGCCGGCGTTAAAGACGCAGAAAGCGGTAAGGAACAGATTTCAGCGGGCTATTTCCGTCTGATCCGCAACTA
TTATCGTTTTGGCTGGGTGATCCCGTATCTGTTCGGCGCATCGCCGGCGATCTGTTCCTCGTTCCTGAAAGGGCGTGAGA
CCGCGTTGCCGTTTGAGCGTACAGAACAGGGTATGTGTTATCTGCCGTATGCCACTTCGCTACGCTTGAGTGACCTGGGT
TACACCAATAAATCACAAAGCAATCTGGGTATTACCTTTAACGACCTGCAAAGTTATGTTGAAGGCTTGAAGCGGGCGAT
TGTTACGCCTTCGGAAGAGTTTGCCAAATTAGGTGTGAAAGATGGCGATCGTCATCTGCAGTTGAACAGTAACGTGCTGC
AGATCGAAAACGAACTCTATGCACCGATCCGGCCGAAGCGGGTGACTAAAAGCGGTGAGACACCGTCAGATGCGCTGCTG
CGCGGCGGTATCGAATACATCGAAGTTCGCTCGCTGGACATCAACCCGTTCTCGCCAATCGGCGTGGATGCGGTACAGGC
ACGTTTCCTGGACCTGTTCCTGATATGGTGTGTGTTGGCCGATGCGCCAGAGATGAGCAGTGATGAATTGCTCTGCACGC
GCAAAAACTGGAACCGGGTGATCCTCGAAGGGCGTAAACCGGGACAGACCATTGGCATAGGTTGCGATGACAGCCGTGAG
CCACTGGCAAAAGTAGGCAAAGCCTTGTTTGACGATTTGCGCCGCGTGGCGGAAGTGTTGGATAGCGAAGCCGGCGATCG
CCAGTATCAGCAGGTGTGTGACGAACTGGTCGCAGCGTTTGACGATCCGGAACTGACGTTCTCGGCCCGTATCCTGAAAG
CGATGAAGGAAGAGGGCACCGGTCGTGTGGGCCTGCAGTTAGCTGAGCAATACCGTCAGATGCTGATTGAAGAGCCGTTG
GAAATCCTGACTGAAACCGAATTGGCGAAGGAGCAGGAAGCCTCATGGCAGCGCCAACGTAACGTAGAAGCCAGTGATAC
GCTAAGCTTTGAAGAGTTCCTGAAGCAACATGGCGGGAGCTGA

Upstream 100 bases:

>100_bases
ATGGCCTGGTGGCATTAACGACGTGAATATAACTCAATATTCTCTCGGGTGCAGATTCAGTATGCTTACGAGTTCTTGTT
TTTAGAAGCGGGAGGTCAAT

Downstream 100 bases:

>100_bases
AAAAGAAAAAGGCCACATCAATGTGGCCAAATATGCATCTCTGATGACAGGGATGATGATAACAAATGCGCGTCTTTCAC
ATATTCAGACTCGCTGAGTT

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MIPDVSQALSWLEAHPHALKGIRRGIERETLRVTEDGKLATTGHPEKLGAALTHHWITTDFAEALLEFITPVDDNLDHLL
TFLRDIHRHVARNLGEERMWPLSMPCFIEAEQDIELAQFGSSNIGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLEFW
QAWAGVKDAESGKEQISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLKGRETALPFERTEQGMCYLPYATSLRLSDLG
YTNKSQSNLGITFNDLQSYVEGLKRAIVTPSEEFAKLGVKDGDRHLQLNSNVLQIENELYAPIRPKRVTKSGETPSDALL
RGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLADAPEMSSDELLCTRKNWNRVILEGRKPGQTIGIGCDDSRE
PLAKVGKALFDDLRRVAEVLDSEAGDRQYQQVCDELVAAFDDPELTFSARILKAMKEEGTGRVGLQLAEQYRQMLIEEPL
EILTETELAKEQEASWQRQRNVEASDTLSFEEFLKQHGGS

Sequences:

>Translated_520_residues
MIPDVSQALSWLEAHPHALKGIRRGIERETLRVTEDGKLATTGHPEKLGAALTHHWITTDFAEALLEFITPVDDNLDHLL
TFLRDIHRHVARNLGEERMWPLSMPCFIEAEQDIELAQFGSSNIGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLEFW
QAWAGVKDAESGKEQISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLKGRETALPFERTEQGMCYLPYATSLRLSDLG
YTNKSQSNLGITFNDLQSYVEGLKRAIVTPSEEFAKLGVKDGDRHLQLNSNVLQIENELYAPIRPKRVTKSGETPSDALL
RGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLADAPEMSSDELLCTRKNWNRVILEGRKPGQTIGIGCDDSRE
PLAKVGKALFDDLRRVAEVLDSEAGDRQYQQVCDELVAAFDDPELTFSARILKAMKEEGTGRVGLQLAEQYRQMLIEEPL
EILTETELAKEQEASWQRQRNVEASDTLSFEEFLKQHGGS
>Mature_520_residues
MIPDVSQALSWLEAHPHALKGIRRGIERETLRVTEDGKLATTGHPEKLGAALTHHWITTDFAEALLEFITPVDDNLDHLL
TFLRDIHRHVARNLGEERMWPLSMPCFIEAEQDIELAQFGSSNIGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLEFW
QAWAGVKDAESGKEQISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLKGRETALPFERTEQGMCYLPYATSLRLSDLG
YTNKSQSNLGITFNDLQSYVEGLKRAIVTPSEEFAKLGVKDGDRHLQLNSNVLQIENELYAPIRPKRVTKSGETPSDALL
RGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLADAPEMSSDELLCTRKNWNRVILEGRKPGQTIGIGCDDSRE
PLAKVGKALFDDLRRVAEVLDSEAGDRQYQQVCDELVAAFDDPELTFSARILKAMKEEGTGRVGLQLAEQYRQMLIEEPL
EILTETELAKEQEASWQRQRNVEASDTLSFEEFLKQHGGS

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789044, Length=518, Percent_Identity=73.9382239382239, Blast_Score=809, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSH1_SERP5 (A8GA13)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477081.1
- ProteinModelPortal:   A8GA13
- SMR:   A8GA13
- STRING:   A8GA13
- GeneID:   5602614
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0847
- eggNOG:   COG2918
- HOGENOM:   HBG289023
- OMA:   FGLIRNY
- ProtClustDB:   PRK02107
- BioCyc:   SPRO399741:SPRO_0847-MONOMER
- HAMAP:   MF_00578
- InterPro:   IPR007370
- InterPro:   IPR006334
- TIGRFAMs:   TIGR01434

Pfam domain/function: PF04262 Glu_cys_ligase

EC number: =6.3.2.2

Molecular weight: Translated: 58781; Mature: 58781

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPDVSQALSWLEAHPHALKGIRRGIERETLRVTEDGKLATTGHPEKLGAALTHHWITTD
CCCCHHHHHHHHHHCHHHHHHHHHCCCHHHEEECCCCCEECCCCHHHHHHHHHHHHHHHH
FAEALLEFITPVDDNLDHLLTFLRDIHRHVARNLGEERMWPLSMPCFIEAEQDIELAQFG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEECCCCCHHHHCC
SSNIGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLEFWQAWAGVKDAESGKEQISAGY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCHHHHHHHHHHHH
FRLIRNYYRFGWVIPYLFGASPAICSSFLKGRETALPFERTEQGMCYLPYATSLRLSDLG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHCCCCEEEECCCCCEEEHHCC
YTNKSQSNLGITFNDLQSYVEGLKRAIVTPSEEFAKLGVKDGDRHLQLNSNVLQIENELY
CCCCCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEECCCEEEECHHHC
APIRPKRVTKSGETPSDALLRGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLA
CCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
DAPEMSSDELLCTRKNWNRVILEGRKPGQTIGIGCDDSREPLAKVGKALFDDLRRVAEVL
CCCCCCCCCEEEECCCCCEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
DSEAGDRQYQQVCDELVAAFDDPELTFSARILKAMKEEGTGRVGLQLAEQYRQMLIEEPL
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EILTETELAKEQEASWQRQRNVEASDTLSFEEFLKQHGGS
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHCCCC
>Mature Secondary Structure
MIPDVSQALSWLEAHPHALKGIRRGIERETLRVTEDGKLATTGHPEKLGAALTHHWITTD
CCCCHHHHHHHHHHCHHHHHHHHHCCCHHHEEECCCCCEECCCCHHHHHHHHHHHHHHHH
FAEALLEFITPVDDNLDHLLTFLRDIHRHVARNLGEERMWPLSMPCFIEAEQDIELAQFG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEECCCCCHHHHCC
SSNIGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLEFWQAWAGVKDAESGKEQISAGY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCHHHHHHHHHHHH
FRLIRNYYRFGWVIPYLFGASPAICSSFLKGRETALPFERTEQGMCYLPYATSLRLSDLG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHCCCCEEEECCCCCEEEHHCC
YTNKSQSNLGITFNDLQSYVEGLKRAIVTPSEEFAKLGVKDGDRHLQLNSNVLQIENELY
CCCCCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEECCCEEEECHHHC
APIRPKRVTKSGETPSDALLRGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLA
CCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
DAPEMSSDELLCTRKNWNRVILEGRKPGQTIGIGCDDSREPLAKVGKALFDDLRRVAEVL
CCCCCCCCCEEEECCCCCEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
DSEAGDRQYQQVCDELVAAFDDPELTFSARILKAMKEEGTGRVGLQLAEQYRQMLIEEPL
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EILTETELAKEQEASWQRQRNVEASDTLSFEEFLKQHGGS
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA