Click here to switch to the map view.

The map label for this gene is truD

Identifier: 157369073

GI number: 157369073

Start: 921377

End: 922423

Strand: Direct

Name: truD

Synonym: Spro_0828

Alternate gene names: 157369073

Gene position: 921377-922423 (Clockwise)

Preceding gene: 157369072

Following gene: 157369074

Centisome position: 16.91

GC content: 56.54

Gene sequence:

>1047_bases
ATGGATATGGCTAACCTGACCTGGCTGCATGGCCAGCCACAGAGCACCGGCGTGTTGAAAGCCAACCCGGAAGATTTTGT
GGTGGTGGAAGACCTGGGCTTTGAGCCGGACGGCGAAGGCGAACATGTGCTGGTGAACATCCGTAAGAACGGTTGTAACA
CTCAGTTTGTCGCCGATTATTTGGCGCGCTTTGCCGGTATTCACGCCCGTTCTGTCAGTTACGCGGGTCTGAAAGATCGC
CATGCGGTGACCGAACAATGGTTCTGCCTGCATCTACCGGGCAAAGAGACACCTGATTTTACTCAGTTTTCACTGGAAGG
CTGCGAAGTGCTGGCCAGCGCCCGCCACCTGCGCAAAATGCGTATAGGCACGCTAAAAGGCAATGCTTTCACGCTGGTGC
TGCGTCATATTTCCGACCGTCAGGATGTCGAACCGCGGCTGCAGGCGATTGCAACCGGCGGCGTGCCAAACTATTTTGGC
AGCCAGCGCTTTGGTCGCGGCGGCAATAACCTGGTGATGGCGCGCCGTTGGGCAAATGATGAGATCCGGGTTAAAGAACG
CAGCAAGCGCAGTTTTTATCTTTCCGCCAGCCGCAGTGCGCTGTTTAATTTGATTGCCAGCCGACGCTTGGCCAACCAGC
AACAACATACCGTGCTGGAAGGCGATGCCTTACAGCTCAGTGGGCGTGGCAGTTGGTTTGTCGCCAAGCCGGAAGAGCTG
GAAGCGCTGCAACAGCGGCTGGATGCCGGCGAACTGATGATTACCGCTCCTTTGCCGGGTGACGGCGAACCAGGCACCGC
CGGTGAAGCGCTGAGTTTTGAACAGCAGTGCTTGCTGGAACAACCGGAACTGATGGCGCTTTTAAAGCGTGAGCGTGTTG
ATCCGGCCCGCCGTGCGTTGCTGTTACAGCCAAAAAACATGCACTGGGAATGGTGGGATGATGCCACCGTTGAACTGCGT
TTTTGGCTGCCGGCCGGCAGTTTCGCCACCAGCGTGGTGCGCGAAATTATGCGGCAGGACAGTAGTGATGCGGATATTAG
TGAGTAA

Upstream 100 bases:

>100_bases
GTCAACGTTAAGGCCACCACCACCGAACAGCTCGGTTTCACCGGGCGCGGCGAGGGCATTGCCTGTGAAGCGGTTGCCTT
GTTGATCAAGGAATAAACGC

Downstream 100 bases:

>100_bases
TAATTAAAAACCTGCTTATGCAGGTTTTTTTTCGTCTGGAGCATGACGACGTAATGAGGTGTGTTTGCCCAACTGCGGTT
TTCGGTGCTAACACGAGGCC

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MDMANLTWLHGQPQSTGVLKANPEDFVVVEDLGFEPDGEGEHVLVNIRKNGCNTQFVADYLARFAGIHARSVSYAGLKDR
HAVTEQWFCLHLPGKETPDFTQFSLEGCEVLASARHLRKMRIGTLKGNAFTLVLRHISDRQDVEPRLQAIATGGVPNYFG
SQRFGRGGNNLVMARRWANDEIRVKERSKRSFYLSASRSALFNLIASRRLANQQQHTVLEGDALQLSGRGSWFVAKPEEL
EALQQRLDAGELMITAPLPGDGEPGTAGEALSFEQQCLLEQPELMALLKRERVDPARRALLLQPKNMHWEWWDDATVELR
FWLPAGSFATSVVREIMRQDSSDADISE

Sequences:

>Translated_348_residues
MDMANLTWLHGQPQSTGVLKANPEDFVVVEDLGFEPDGEGEHVLVNIRKNGCNTQFVADYLARFAGIHARSVSYAGLKDR
HAVTEQWFCLHLPGKETPDFTQFSLEGCEVLASARHLRKMRIGTLKGNAFTLVLRHISDRQDVEPRLQAIATGGVPNYFG
SQRFGRGGNNLVMARRWANDEIRVKERSKRSFYLSASRSALFNLIASRRLANQQQHTVLEGDALQLSGRGSWFVAKPEEL
EALQQRLDAGELMITAPLPGDGEPGTAGEALSFEQQCLLEQPELMALLKRERVDPARRALLLQPKNMHWEWWDDATVELR
FWLPAGSFATSVVREIMRQDSSDADISE
>Mature_348_residues
MDMANLTWLHGQPQSTGVLKANPEDFVVVEDLGFEPDGEGEHVLVNIRKNGCNTQFVADYLARFAGIHARSVSYAGLKDR
HAVTEQWFCLHLPGKETPDFTQFSLEGCEVLASARHLRKMRIGTLKGNAFTLVLRHISDRQDVEPRLQAIATGGVPNYFG
SQRFGRGGNNLVMARRWANDEIRVKERSKRSFYLSASRSALFNLIASRRLANQQQHTVLEGDALQLSGRGSWFVAKPEEL
EALQQRLDAGELMITAPLPGDGEPGTAGEALSFEQQCLLEQPELMALLKRERVDPARRALLLQPKNMHWEWWDDATVELR
FWLPAGSFATSVVREIMRQDSSDADISE

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Escherichia coli, GI1789102, Length=348, Percent_Identity=64.9425287356322, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_SERP5 (A8G9Z4)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477062.1
- ProteinModelPortal:   A8G9Z4
- SMR:   A8G9Z4
- STRING:   A8G9Z4
- GeneID:   5603033
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0828
- eggNOG:   COG0585
- HOGENOM:   HBG541221
- OMA:   ALTRQWF
- ProtClustDB:   PRK00984
- BioCyc:   SPRO399741:SPRO_0828-MONOMER
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 39069; Mature: 39069

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 79-79 BINDING 26-26 BINDING 128-128 BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMANLTWLHGQPQSTGVLKANPEDFVVVEDLGFEPDGEGEHVLVNIRKNGCNTQFVADY
CCCCCEEEECCCCCCCCEEEECCCCEEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHH
LARFAGIHARSVSYAGLKDRHAVTEQWFCLHLPGKETPDFTQFSLEGCEVLASARHLRKM
HHHHHCCHHCCEECCCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RIGTLKGNAFTLVLRHISDRQDVEPRLQAIATGGVPNYFGSQRFGRGGNNLVMARRWAND
HHEEECCCEEEEEEEHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
EIRVKERSKRSFYLSASRSALFNLIASRRLANQQQHTVLEGDALQLSGRGSWFVAKPEEL
CEEEEECCCCEEEEECHHHHHHHHHHHHHHCCCHHHEEECCCEEEECCCCCEEEECCHHH
EALQQRLDAGELMITAPLPGDGEPGTAGEALSFEQQCLLEQPELMALLKRERVDPARRAL
HHHHHHCCCCCEEEEECCCCCCCCCCCCCHHCHHHHHHHCCHHHHHHHHHHCCCHHHHEE
LLQPKNMHWEWWDDATVELRFWLPAGSFATSVVREIMRQDSSDADISE
EECCCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDMANLTWLHGQPQSTGVLKANPEDFVVVEDLGFEPDGEGEHVLVNIRKNGCNTQFVADY
CCCCCEEEECCCCCCCCEEEECCCCEEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHH
LARFAGIHARSVSYAGLKDRHAVTEQWFCLHLPGKETPDFTQFSLEGCEVLASARHLRKM
HHHHHCCHHCCEECCCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RIGTLKGNAFTLVLRHISDRQDVEPRLQAIATGGVPNYFGSQRFGRGGNNLVMARRWAND
HHEEECCCEEEEEEEHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
EIRVKERSKRSFYLSASRSALFNLIASRRLANQQQHTVLEGDALQLSGRGSWFVAKPEEL
CEEEEECCCCEEEEECHHHHHHHHHHHHHHCCCHHHEEECCCEEEECCCCCEEEECCHHH
EALQQRLDAGELMITAPLPGDGEPGTAGEALSFEQQCLLEQPELMALLKRERVDPARRAL
HHHHHHCCCCCEEEEECCCCCCCCCCCCCHHCHHHHHHHCCHHHHHHHHHHCCCHHHHEE
LLQPKNMHWEWWDDATVELRFWLPAGSFATSVVREIMRQDSSDADISE
EECCCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA