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The map label for this gene is ispD

Identifier: 157369071

GI number: 157369071

Start: 920181

End: 920888

Strand: Direct

Name: ispD

Synonym: Spro_0826

Alternate gene names: 157369071

Gene position: 920181-920888 (Clockwise)

Preceding gene: 157369070

Following gene: 157369072

Centisome position: 16.89

GC content: 59.46

Gene sequence:

>708_bases
ATGAATCACTCCGCAGGAACCTTTCCACCGGTGATTGCCGTCCTGCCGGCTGCCGGTATCGGCAGCCGTATGCAGGCGGA
TTGCCCGAAGCAGTATTTAACCATCGGCCACCAAACCATTCTCGAGCACGCGATCCATGCGCTATTGCGCCATCCGCGTA
TTCCGCAGGTGATTGTGGCGATCGGTCCCGATGACCAGCAGTTCCACCAGTTACCGATTGCCAAAGATCCCCGCGTGCGG
GTCACCGTCGGCGGTCAGCAACGCGCCGATTCGGTGATGGCCGGGTTGCAACTGGCCGGCGAGGCCAAATGGGTGCTGGT
ACATGACGCGGCAAGACCCTGCCTGCATGCCGAAGATCTGGAACGCCTGCTGGCGATCACCGAACACAGCGAGGTGGGGG
GCATTTTGGCCGCGCCGGTGCGTGATACCATGAAGCGTGCCGAACCTGGTCGCAGTACTATCTCACACACCGTTGAACGC
CAGGATCTGTGGCATGCGTTAACTCCGCAACTGTTCCCGCTGGCGTTGCTCAAGCTCTGTCTGCAGCGTGCGATGGATGA
CGGTGCGACGGTCACCGATGAGGCTTCTGCCCTGGAGCATTGTGGCTATCATCCGCTGCTGGTCAGTGGACGCTCGGACA
ATATTAAAGTCACGCGCCCGGAAGATTTGGCGCTGGCGGCCTTTTACTTAACTCAGTTGGACAATTAA

Upstream 100 bases:

>100_bases
GAGCATGATCAAGCCCGGTGAGACTTTCTATCGTCTGGTTCCTGACCAATCCAGACGCAACGCGGCGTCCTCCTCGCAAA
ATAACCTACAAAAGTAACGC

Downstream 100 bases:

>100_bases
GGAGCGCATCATGCGTATCGGTCACGGTTTTGACGTACATAAATTTGGTGGCGAAGGGCCGCTGGTGATCGGTGGTGTTC
GTATCCCTTATGAAAAAGGT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MNHSAGTFPPVIAVLPAAGIGSRMQADCPKQYLTIGHQTILEHAIHALLRHPRIPQVIVAIGPDDQQFHQLPIAKDPRVR
VTVGGQQRADSVMAGLQLAGEAKWVLVHDAARPCLHAEDLERLLAITEHSEVGGILAAPVRDTMKRAEPGRSTISHTVER
QDLWHALTPQLFPLALLKLCLQRAMDDGATVTDEASALEHCGYHPLLVSGRSDNIKVTRPEDLALAAFYLTQLDN

Sequences:

>Translated_235_residues
MNHSAGTFPPVIAVLPAAGIGSRMQADCPKQYLTIGHQTILEHAIHALLRHPRIPQVIVAIGPDDQQFHQLPIAKDPRVR
VTVGGQQRADSVMAGLQLAGEAKWVLVHDAARPCLHAEDLERLLAITEHSEVGGILAAPVRDTMKRAEPGRSTISHTVER
QDLWHALTPQLFPLALLKLCLQRAMDDGATVTDEASALEHCGYHPLLVSGRSDNIKVTRPEDLALAAFYLTQLDN
>Mature_235_residues
MNHSAGTFPPVIAVLPAAGIGSRMQADCPKQYLTIGHQTILEHAIHALLRHPRIPQVIVAIGPDDQQFHQLPIAKDPRVR
VTVGGQQRADSVMAGLQLAGEAKWVLVHDAARPCLHAEDLERLLAITEHSEVGGILAAPVRDTMKRAEPGRSTISHTVER
QDLWHALTPQLFPLALLKLCLQRAMDDGATVTDEASALEHCGYHPLLVSGRSDNIKVTRPEDLALAAFYLTQLDN

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=241, Percent_Identity=28.2157676348548, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789104, Length=228, Percent_Identity=68.4210526315789, Blast_Score=331, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_SERP5 (A8G9Z2)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477060.1
- ProteinModelPortal:   A8G9Z2
- SMR:   A8G9Z2
- STRING:   A8G9Z2
- GeneID:   5603080
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0826
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- BioCyc:   SPRO399741:SPRO_0826-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25623; Mature: 25623

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSAGTFPPVIAVLPAAGIGSRMQADCPKQYLTIGHQTILEHAIHALLRHPRIPQVIVA
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IGPDDQQFHQLPIAKDPRVRVTVGGQQRADSVMAGLQLAGEAKWVLVHDAARPCLHAEDL
ECCCCHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHH
ERLLAITEHSEVGGILAAPVRDTMKRAEPGRSTISHTVERQDLWHALTPQLFPLALLKLC
HHHHHHHCCCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
LQRAMDDGATVTDEASALEHCGYHPLLVSGRSDNIKVTRPEDLALAAFYLTQLDN
HHHHHCCCCCCCCHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNHSAGTFPPVIAVLPAAGIGSRMQADCPKQYLTIGHQTILEHAIHALLRHPRIPQVIVA
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IGPDDQQFHQLPIAKDPRVRVTVGGQQRADSVMAGLQLAGEAKWVLVHDAARPCLHAEDL
ECCCCHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHH
ERLLAITEHSEVGGILAAPVRDTMKRAEPGRSTISHTVERQDLWHALTPQLFPLALLKLC
HHHHHHHCCCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
LQRAMDDGATVTDEASALEHCGYHPLLVSGRSDNIKVTRPEDLALAAFYLTQLDN
HHHHHCCCCCCCCHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA