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The map label for this gene is mraW

Identifier: 157368998

GI number: 157368998

Start: 840090

End: 841031

Strand: Direct

Name: mraW

Synonym: Spro_0753

Alternate gene names: 157368998

Gene position: 840090-841031 (Clockwise)

Preceding gene: 157368997

Following gene: 157368999

Centisome position: 15.42

GC content: 57.96

Gene sequence:

>942_bases
ATGTTGGAAAATTATAAACACACCACCGTACTGTTGGATGAGGCGGTTAACGGCCTTAATATTCGCAGTAACGGCACATA
TATCGACGGTACTTTTGGCCGCGGTGGCCATTCGCGTCTGATTCTGTCCCAGTTGGGGCCGGAAGGGCGTTTGCTGGCAA
TTGACCGCGACCCACAGGCGATTGCAGCCGCCAAGTCTATTGAAGATCCTCGTTTTAGCATCGTACACGGCCCCTTTTCA
GATTTGTCACACTATGTGCGGGAACGCGAACTGGTGGGGCAGATTGACGGCATTTTACTCGATCTGGGCGTTTCTTCGCC
GCAGTTGGATGACGCAGAGCGCGGTTTCTCCTTCATGCGTGACGGGCCGCTGGACATGCGTATGGACTCCTCAAGCGGTC
AGTCAGCCTCCGAGTGGCTAATGAAAGCGGAAGCCGACGACATTGCCTGGGTGTTGAAAACCTTTGGCGAAGAGCGTTTC
GCCAAGCGTATCGCTCGCGCCATCGTGGAAAGAAACCAGGTCGACCCGATGACTCGCACCAAACAGCTGGCCGATTTGAT
CGCCAACGCCAGCCCGTTCCGGGACAAGCACAAACACCCGGCAACCCGCAGTTTCCAGGCGATCCGCATCTACATAAACA
GTGAGTTGGAAGAGATCGAACGTGCGCTGGACGGCGCGCTGGAAGTGCTGGCTCCGCAGGGGCGCCTGTCGGTGATCAGT
TTCCACTCGCTGGAAGACCGCATCGTTAAACGCTTTATGCGCCACCACAGCCGCGGCGCTCAGGTGCCGGCCGGTATTCC
GCTGACCGAAGCGCAATTGCGCAGTATGGGCGGACGCACGCTGAAAGCGCTGGGCAAGATGATGCCTGGGGATGCCGAAG
TGGCGGAAAACCCGCGCGCACGTAGCTCGGTGCTGCGTATTGCCGAGAGGATGCCCACGTGA

Upstream 100 bases:

>100_bases
ATGAACAGACCTGGTATCAACAAGTCAAGGATGATATTGACGCAGAACAGTCGACTCAGGAACCGTTGTCTGAGCGGCTA
CAGGACTTGTCGCTATAAGC

Downstream 100 bases:

>100_bases
TTGGTAATGAGCGTCACGGCCTGGTTGGGGTGATTGGCGGCGATCTGCTGCGTAACGCCAAGATCCCGTTGATTTTGCTG
ATTGCCTCGCTGGTGTCAGC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLENYKHTTVLLDEAVNGLNIRSNGTYIDGTFGRGGHSRLILSQLGPEGRLLAIDRDPQAIAAAKSIEDPRFSIVHGPFS
DLSHYVRERELVGQIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDSSSGQSASEWLMKAEADDIAWVLKTFGEERF
AKRIARAIVERNQVDPMTRTKQLADLIANASPFRDKHKHPATRSFQAIRIYINSELEEIERALDGALEVLAPQGRLSVIS
FHSLEDRIVKRFMRHHSRGAQVPAGIPLTEAQLRSMGGRTLKALGKMMPGDAEVAENPRARSSVLRIAERMPT

Sequences:

>Translated_313_residues
MLENYKHTTVLLDEAVNGLNIRSNGTYIDGTFGRGGHSRLILSQLGPEGRLLAIDRDPQAIAAAKSIEDPRFSIVHGPFS
DLSHYVRERELVGQIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDSSSGQSASEWLMKAEADDIAWVLKTFGEERF
AKRIARAIVERNQVDPMTRTKQLADLIANASPFRDKHKHPATRSFQAIRIYINSELEEIERALDGALEVLAPQGRLSVIS
FHSLEDRIVKRFMRHHSRGAQVPAGIPLTEAQLRSMGGRTLKALGKMMPGDAEVAENPRARSSVLRIAERMPT
>Mature_313_residues
MLENYKHTTVLLDEAVNGLNIRSNGTYIDGTFGRGGHSRLILSQLGPEGRLLAIDRDPQAIAAAKSIEDPRFSIVHGPFS
DLSHYVRERELVGQIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDSSSGQSASEWLMKAEADDIAWVLKTFGEERF
AKRIARAIVERNQVDPMTRTKQLADLIANASPFRDKHKHPATRSFQAIRIYINSELEEIERALDGALEVLAPQGRLSVIS
FHSLEDRIVKRFMRHHSRGAQVPAGIPLTEAQLRSMGGRTLKALGKMMPGDAEVAENPRARSSVLRIAERMPT

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=347, Percent_Identity=37.1757925072046, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI165377202, Length=192, Percent_Identity=40.1041666666667, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI1786270, Length=310, Percent_Identity=80, Blast_Score=520, Evalue=1e-149,
Organism=Drosophila melanogaster, GI62472493, Length=328, Percent_Identity=41.4634146341463, Blast_Score=207, Evalue=5e-54,
Organism=Drosophila melanogaster, GI28571637, Length=328, Percent_Identity=41.4634146341463, Blast_Score=207, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_SERP5 (A8G9R9)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476987.1
- ProteinModelPortal:   A8G9R9
- SMR:   A8G9R9
- STRING:   A8G9R9
- GeneID:   5604771
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0753
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   SPRO399741:SPRO_0753-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34790; Mature: 34790

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 79-79 BINDING 101-101 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENYKHTTVLLDEAVNGLNIRSNGTYIDGTFGRGGHSRLILSQLGPEGRLLAIDRDPQA
CCCCCCHHHEEHHHHCCCCEECCCCCEEECCCCCCCCCEEHHHHCCCCCCEEEECCCCHH
IAAAKSIEDPRFSIVHGPFSDLSHYVRERELVGQIDGILLDLGVSSPQLDDAERGFSFMR
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHCHHHHC
DGPLDMRMDSSSGQSASEWLMKAEADDIAWVLKTFGEERFAKRIARAIVERNQVDPMTRT
CCCCCEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KQLADLIANASPFRDKHKHPATRSFQAIRIYINSELEEIERALDGALEVLAPQGRLSVIS
HHHHHHHHCCCCCHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEE
FHSLEDRIVKRFMRHHSRGAQVPAGIPLTEAQLRSMGGRTLKALGKMMPGDAEVAENPRA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHCCCHH
RSSVLRIAERMPT
HHHHHHHHHHCCC
>Mature Secondary Structure
MLENYKHTTVLLDEAVNGLNIRSNGTYIDGTFGRGGHSRLILSQLGPEGRLLAIDRDPQA
CCCCCCHHHEEHHHHCCCCEECCCCCEEECCCCCCCCCEEHHHHCCCCCCEEEECCCCHH
IAAAKSIEDPRFSIVHGPFSDLSHYVRERELVGQIDGILLDLGVSSPQLDDAERGFSFMR
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHCHHHHC
DGPLDMRMDSSSGQSASEWLMKAEADDIAWVLKTFGEERFAKRIARAIVERNQVDPMTRT
CCCCCEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KQLADLIANASPFRDKHKHPATRSFQAIRIYINSELEEIERALDGALEVLAPQGRLSVIS
HHHHHHHHCCCCCHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEE
FHSLEDRIVKRFMRHHSRGAQVPAGIPLTEAQLRSMGGRTLKALGKMMPGDAEVAENPRA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHCCCHH
RSSVLRIAERMPT
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA