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The map label for this gene is ilvI [H]

Identifier: 157368993

GI number: 157368993

Start: 834851

End: 836569

Strand: Direct

Name: ilvI [H]

Synonym: Spro_0748

Alternate gene names: 157368993

Gene position: 834851-836569 (Clockwise)

Preceding gene: 157368992

Following gene: 157368994

Centisome position: 15.32

GC content: 57.01

Gene sequence:

>1719_bases
ATGGAGATGTTGTCAGGAGCTGAGATGGTCGTCCGATCGTTGATCGATCAAGGCGTTAAGCATGTATTCGGTTATCCGGG
CGGGGCGGTACTCGATATCTACGACGCCCTGCATACGGTCGGAGGGATCGACCACATATTGGTGCGCCATGAGCAAGGTG
CGGTGCATATGGCCGATGGCTATGCGCGTGCGACCGGTGAAGTGGGGGTGGTGCTGGTGACCTCTGGCCCTGGAGCGACC
AATGCGATCACCGGCATTGCAACCGCCTATATGGACTCCATTCCGATGGTGGTGCTTTCCGGTCAGGTTCCCAGCTCGCT
GATTGGCTATGATGCCTTCCAGGAGTGCGACATGGTCGGGATTTCACGCCCGGTGGTGAAACACAGTTTCCTGGTCAAAC
GTACCGAAGACATTCCCGGCATACTGAAAAAGGCTTTTTACCTGGCCTCAACCGGCCGCCCTGGGCCGGTGGTGATCGAT
TTGCCGAAAGACATCGTGGGCCCGGCGGTGAGAATGCCTTACGCCTATCCGCAGGACGTGAGCATGCGTTCCTATAACCC
GACGGTGCAAGGGCACCGCGGGCAGATCAAACGGGCACTGCACACCATTTTGACGGCCAAAAAGCCCGTTATGTACGTCG
GTGGCGGGGCGATCAACTCGGCCTGTGACGCAGAGCTGCTGGAACTGGCGGAGAAGCTGAATCTGCCTGTCACCTGCACC
CTGATGGGGTTGGGGGCCTTCCCCGGCACGCATCGCCAGAGCGTGGGTATGCTCGGCATGCATGGGACTTATGAAGCCAA
CAAAACCATGCATAACTCTGACGTAATCTTTGCCGTCGGCGTGCGTTTTGACGATCGCACCACCAATAACCTGGCCAAAT
ACTGCCCGAACGCTACCGTGCTGCATATCGACATTGACCCTACCTCAATTTCCAAGACGGTAGACGCCGATATTCCTATT
GTCGGCGATGCCAAACAGGTGTTGACCCAAATGCTGGAGCTGCTGGCGCAGGAAGATAAAGCGCAGGATCACGACGCGTT
GCGCGACTGGTGGCAGGCTATCGAACAGTGGCGTGCGCGTGATTGCCTGGGTTACGACAAAAACAGCGGCACCATTAAGC
CGCAGGCAGTGATCGAAACCCTGCATCGGCTGACCAGAGGGGACGCTTATGTCACTTCCGATGTCGGCCAGCACCAGATG
TTCGCGGCACTCTACTATCCGTTCGACAAACCACGCCGTTGGATCAACTCCGGCGGATTGGGCACCATGGGCTTTGGTCT
GCCGGCGGCGCTGGGCGTGAAGCTGGCGCTGCCTGATGAAACCGTGGTGTGTGTCACCGGCGACGGCAGCATCCAGATGA
ACATTCAGGAACTGTCCACCGCGTTGCAATACAACCTGCCGGTGATCGTAGTGAATCTTAACAACCGCTATCTGGGTATG
GTGAAGCAGTGGCAGGACATGATTTATTCCGGCCGTCACTCCCAGTCTTATATGGATTCCCTGCCGGACTTCGTCAAACT
GGCGGAGGCTTACGGCCACGTGGGCATTGCCATTCGCACGCCGGATGAACTGGAAAGCAAACTGGCTCAGGCGCTGGCCG
AGAAAGAGCGCCTGGTATTTGTCGACGTGACCGTCGATGAAACTGAACATGTTTACCCAATGCAGATTCGTGGTGGAAGC
ATGGACGAAATGTGGTTAAGCAAAACGGAGAGGACCTGA

Upstream 100 bases:

>100_bases
AATGGGTTATAGTCAATCCATAAAAATTTTGGGGTATTGCCCCACAACAGGTGCCACGTTCCGGTGCCAGATAAGAATAA
GTTAGCCTGGAGGCAAACCC

Downstream 100 bases:

>100_bases
TCATGCGCCGTATTTTATCTGTATTGCTGGAAAATGAATCCGGTGCGTTATCGCGTGTGGTGGGTTTGTTCTCTCAGCGC
GGTTACAACATTGAAAGCCT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MEMLSGAEMVVRSLIDQGVKHVFGYPGGAVLDIYDALHTVGGIDHILVRHEQGAVHMADGYARATGEVGVVLVTSGPGAT
NAITGIATAYMDSIPMVVLSGQVPSSLIGYDAFQECDMVGISRPVVKHSFLVKRTEDIPGILKKAFYLASTGRPGPVVID
LPKDIVGPAVRMPYAYPQDVSMRSYNPTVQGHRGQIKRALHTILTAKKPVMYVGGGAINSACDAELLELAEKLNLPVTCT
LMGLGAFPGTHRQSVGMLGMHGTYEANKTMHNSDVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVDADIPI
VGDAKQVLTQMLELLAQEDKAQDHDALRDWWQAIEQWRARDCLGYDKNSGTIKPQAVIETLHRLTRGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKLALPDETVVCVTGDGSIQMNIQELSTALQYNLPVIVVNLNNRYLGM
VKQWQDMIYSGRHSQSYMDSLPDFVKLAEAYGHVGIAIRTPDELESKLAQALAEKERLVFVDVTVDETEHVYPMQIRGGS
MDEMWLSKTERT

Sequences:

>Translated_572_residues
MEMLSGAEMVVRSLIDQGVKHVFGYPGGAVLDIYDALHTVGGIDHILVRHEQGAVHMADGYARATGEVGVVLVTSGPGAT
NAITGIATAYMDSIPMVVLSGQVPSSLIGYDAFQECDMVGISRPVVKHSFLVKRTEDIPGILKKAFYLASTGRPGPVVID
LPKDIVGPAVRMPYAYPQDVSMRSYNPTVQGHRGQIKRALHTILTAKKPVMYVGGGAINSACDAELLELAEKLNLPVTCT
LMGLGAFPGTHRQSVGMLGMHGTYEANKTMHNSDVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVDADIPI
VGDAKQVLTQMLELLAQEDKAQDHDALRDWWQAIEQWRARDCLGYDKNSGTIKPQAVIETLHRLTRGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKLALPDETVVCVTGDGSIQMNIQELSTALQYNLPVIVVNLNNRYLGM
VKQWQDMIYSGRHSQSYMDSLPDFVKLAEAYGHVGIAIRTPDELESKLAQALAEKERLVFVDVTVDETEHVYPMQIRGGS
MDEMWLSKTERT
>Mature_572_residues
MEMLSGAEMVVRSLIDQGVKHVFGYPGGAVLDIYDALHTVGGIDHILVRHEQGAVHMADGYARATGEVGVVLVTSGPGAT
NAITGIATAYMDSIPMVVLSGQVPSSLIGYDAFQECDMVGISRPVVKHSFLVKRTEDIPGILKKAFYLASTGRPGPVVID
LPKDIVGPAVRMPYAYPQDVSMRSYNPTVQGHRGQIKRALHTILTAKKPVMYVGGGAINSACDAELLELAEKLNLPVTCT
LMGLGAFPGTHRQSVGMLGMHGTYEANKTMHNSDVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVDADIPI
VGDAKQVLTQMLELLAQEDKAQDHDALRDWWQAIEQWRARDCLGYDKNSGTIKPQAVIETLHRLTRGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKLALPDETVVCVTGDGSIQMNIQELSTALQYNLPVIVVNLNNRYLGM
VKQWQDMIYSGRHSQSYMDSLPDFVKLAEAYGHVGIAIRTPDELESKLAQALAEKERLVFVDVTVDETEHVYPMQIRGGS
MDEMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=590, Percent_Identity=27.1186440677966, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI21361361, Length=513, Percent_Identity=26.9005847953216, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=84.4947735191638, Blast_Score=1016, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=40.7473309608541, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1786717, Length=567, Percent_Identity=35.978835978836, Blast_Score=336, Evalue=3e-93,
Organism=Escherichia coli, GI1787096, Length=558, Percent_Identity=29.5698924731183, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1788716, Length=576, Percent_Identity=25.5208333333333, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=579, Percent_Identity=26.5975820379965, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17531299, Length=579, Percent_Identity=26.5975820379965, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17542570, Length=598, Percent_Identity=24.9163879598662, Blast_Score=144, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=584, Percent_Identity=42.8082191780822, Blast_Score=450, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320816, Length=483, Percent_Identity=25.879917184265, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6323163, Length=474, Percent_Identity=24.0506329113924, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320123, Length=539, Percent_Identity=22.6345083487941, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321524, Length=496, Percent_Identity=21.7741935483871, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=492, Percent_Identity=23.5772357723577, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19922626, Length=592, Percent_Identity=25.8445945945946, Blast_Score=176, Evalue=3e-44,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62518; Mature: 62518

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGAEMVVRSLIDQGVKHVFGYPGGAVLDIYDALHTVGGIDHILVRHEQGAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEECC
YARATGEVGVVLVTSGPGATNAITGIATAYMDSIPMVVLSGQVPSSLIGYDAFQECDMVG
CEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHCCHHHHHCCCCC
ISRPVVKHSFLVKRTEDIPGILKKAFYLASTGRPGPVVIDLPKDIVGPAVRMPYAYPQDV
CCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCHHCCCCCCCCCC
SMRSYNPTVQGHRGQIKRALHTILTAKKPVMYVGGGAINSACDAELLELAEKLNLPVTCT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
LMGLGAFPGTHRQSVGMLGMHGTYEANKTMHNSDVIFAVGVRFDDRTTNNLAKYCPNATV
EEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHIDIDPTSISKTVDADIPIVGDAKQVLTQMLELLAQEDKAQDHDALRDWWQAIEQWRAR
EEEECCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
DCLGYDKNSGTIKPQAVIETLHRLTRGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
HCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHEEHHEECCCHHHHHHHCCCCC
GTMGFGLPAALGVKLALPDETVVCVTGDGSIQMNIQELSTALQYNLPVIVVNLNNRYLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCCHHHH
VKQWQDMIYSGRHSQSYMDSLPDFVKLAEAYGHVGIAIRTPDELESKLAQALAEKERLVF
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCCCEEE
VDVTVDETEHVYPMQIRGGSMDEMWLSKTERT
EEEEECCCCCEEEEEECCCCCHHHHHHHCCCC
>Mature Secondary Structure
MEMLSGAEMVVRSLIDQGVKHVFGYPGGAVLDIYDALHTVGGIDHILVRHEQGAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEECC
YARATGEVGVVLVTSGPGATNAITGIATAYMDSIPMVVLSGQVPSSLIGYDAFQECDMVG
CEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHCCHHHHHCCCCC
ISRPVVKHSFLVKRTEDIPGILKKAFYLASTGRPGPVVIDLPKDIVGPAVRMPYAYPQDV
CCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCHHCCCCCCCCCC
SMRSYNPTVQGHRGQIKRALHTILTAKKPVMYVGGGAINSACDAELLELAEKLNLPVTCT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
LMGLGAFPGTHRQSVGMLGMHGTYEANKTMHNSDVIFAVGVRFDDRTTNNLAKYCPNATV
EEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHIDIDPTSISKTVDADIPIVGDAKQVLTQMLELLAQEDKAQDHDALRDWWQAIEQWRAR
EEEECCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
DCLGYDKNSGTIKPQAVIETLHRLTRGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
HCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHEEHHEECCCHHHHHHHCCCCC
GTMGFGLPAALGVKLALPDETVVCVTGDGSIQMNIQELSTALQYNLPVIVVNLNNRYLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCCHHHH
VKQWQDMIYSGRHSQSYMDSLPDFVKLAEAYGHVGIAIRTPDELESKLAQALAEKERLVF
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCCCEEE
VDVTVDETEHVYPMQIRGGSMDEMWLSKTERT
EEEEECCCCCEEEEEECCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]