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The map label for this gene is carA [H]

Identifier: 157368958

GI number: 157368958

Start: 788062

End: 789210

Strand: Direct

Name: carA [H]

Synonym: Spro_0713

Alternate gene names: 157368958

Gene position: 788062-789210 (Clockwise)

Preceding gene: 157368957

Following gene: 157368959

Centisome position: 14.46

GC content: 58.05

Gene sequence:

>1149_bases
TTGATTAAGTCAGCGCTATTGGTTCTCGAAGACGGAACCCAATTCCACGGTCGGGCCATCGGGGCAGAGGGTACGGCAGT
AGGAGAAGTGGTCTTCAATACGTCGATGACCGGTTATCAAGAAATCCTCACTGATCCTTCCTACTCCCGCCAGATCGTTA
CTCTTACTTATCCTCATATCGGCAATGTCGGCACCAATGCTGCTGACGAAGAATCCTCCGCAGTACACGCCCAAGGCCTC
GTCATTCGCGACCTCCCACTGATTGCCAGCAACTACCGCAACGAAGAAGGCCTGTCTGACTACCTGAAGCGTCACAACAT
CGTGGCGATCGCCGATATTGATACCCGCAAGCTGACCCGTCTGCTGCGTGAGAAGGGTGCACAGAACGGCTGCATCATTG
CTGCCGATACCCCGGATGCAGCCCTGGCCTTGGCCAAGGCCAAAGGTTTCCCGGGCTTGAAAGGCATGGATCTGGCGAAA
GAAGTGACCACCCAGGAAGCTTACAGCTGGCAGCAAGGCAGCTGGACGCTGGAAGGCGAACTGCCGGAAGCCAAAGCGGC
CTCCGAGCTGCCATTCCACGTGGTGGCTTACGACTTCGGCGCCAAGCGCAACATCCTGCGTATGCTGGTGGATCGCGGCT
GCCGCCTGACGGTAGTGCCGGCACAGACCCCGGCAGATGAAGTGTTGAAGATGGCTCCGGACGGCATTTTCCTGTCCAAC
GGCCCGGGTGACCCGGAGCCTTGTGATTACGCCATCACCGCCATCAAGCAGTTCCTGGAAACCGACATTCCGGTGTTTGG
CATCTGCCTCGGCCACCAGTTGTTGGCACTGGCCAGCGGCGCGAAAACCGTGAAGATGAAGCTCGGCCACCACGGCGGCA
ACCACCCGGTAAAAGACCTGGATAAAGACTGCGTCATGATCACCGCGCAGAACCATGGTTTTGCCGTGGACGAAAATAAC
CTGCCGGCGGAACTGCGCGTAACGCACAAATCCTTGTTTGACCACACGGTGCAGGGCATTCACCGCACCGACAAGGCGGC
GTTCAGCTTCCAGGGCCACCCGGAAGCCAGCCCGGGCCCACACGATGCCGCGCCGCTGTTCGATCACTTTATCGAACTGA
TCGAGACTTACCGTTCTAACGCCAAATAA

Upstream 100 bases:

>100_bases
TGCCAAAAATTGACCTTGAGGGCGGTTTTTGCATTGATTTAGATCGCGATATCTGAATTAATATGCAAATATTGTGATTG
ATTATTCCTTGGAGGGTGTT

Downstream 100 bases:

>100_bases
TCAGGAGCAGTAAAAATGCCAAAACGTACAGACATAAAAAGCATCCTGATCCTCGGCGCTGGCCCGATTGTTATCGGCCA
GGCGTGTGAGTTCGACTACT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNAADEESSAVHAQGL
VIRDLPLIASNYRNEEGLSDYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIAADTPDAALALAKAKGFPGLKGMDLAK
EVTTQEAYSWQQGSWTLEGELPEAKAASELPFHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTPADEVLKMAPDGIFLSN
GPGDPEPCDYAITAIKQFLETDIPVFGICLGHQLLALASGAKTVKMKLGHHGGNHPVKDLDKDCVMITAQNHGFAVDENN
LPAELRVTHKSLFDHTVQGIHRTDKAAFSFQGHPEASPGPHDAAPLFDHFIELIETYRSNAK

Sequences:

>Translated_382_residues
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNAADEESSAVHAQGL
VIRDLPLIASNYRNEEGLSDYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIAADTPDAALALAKAKGFPGLKGMDLAK
EVTTQEAYSWQQGSWTLEGELPEAKAASELPFHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTPADEVLKMAPDGIFLSN
GPGDPEPCDYAITAIKQFLETDIPVFGICLGHQLLALASGAKTVKMKLGHHGGNHPVKDLDKDCVMITAQNHGFAVDENN
LPAELRVTHKSLFDHTVQGIHRTDKAAFSFQGHPEASPGPHDAAPLFDHFIELIETYRSNAK
>Mature_382_residues
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNAADEESSAVHAQGL
VIRDLPLIASNYRNEEGLSDYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIAADTPDAALALAKAKGFPGLKGMDLAK
EVTTQEAYSWQQGSWTLEGELPEAKAASELPFHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTPADEVLKMAPDGIFLSN
GPGDPEPCDYAITAIKQFLETDIPVFGICLGHQLLALASGAKTVKMKLGHHGGNHPVKDLDKDCVMITAQNHGFAVDENN
LPAELRVTHKSLFDHTVQGIHRTDKAAFSFQGHPEASPGPHDAAPLFDHFIELIETYRSNAK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=37.696335078534, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI169790915, Length=385, Percent_Identity=36.1038961038961, Blast_Score=224, Evalue=8e-59,
Organism=Homo sapiens, GI21361331, Length=385, Percent_Identity=36.1038961038961, Blast_Score=224, Evalue=9e-59,
Organism=Escherichia coli, GI1786215, Length=382, Percent_Identity=88.7434554973822, Blast_Score=704, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193204318, Length=389, Percent_Identity=35.9897172236504, Blast_Score=228, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6324878, Length=390, Percent_Identity=39.2307692307692, Blast_Score=236, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6322331, Length=396, Percent_Identity=35.8585858585859, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=39.0745501285347, Blast_Score=225, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=39.0745501285347, Blast_Score=224, Evalue=5e-59,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41380; Mature: 41380

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI
CCCCEEEEEECCCCCCCEEECCCCCEEEHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNVGTNAADEESSAVHAQGLVIRDLPLIASNYRNEEGLSDYLKRHNIVAIADIDTRKLTR
CCCCCCCCCCCCCCHHHCCEEEECCCHHHHCCCCCCHHHHHHHHCCEEEEEECCHHHHHH
LLREKGAQNGCIIAADTPDAALALAKAKGFPGLKGMDLAKEVTTQEAYSWQQGSWTLEGE
HHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECC
LPEAKAASELPFHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTPADEVLKMAPDGIFLSN
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCEEECC
GPGDPEPCDYAITAIKQFLETDIPVFGICLGHQLLALASGAKTVKMKLGHHGGNHPVKDL
CCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHC
DKDCVMITAQNHGFAVDENNLPAELRVTHKSLFDHTVQGIHRTDKAAFSFQGHPEASPGP
CCCEEEEEECCCCEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
HDAAPLFDHFIELIETYRSNAK
CCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI
CCCCEEEEEECCCCCCCEEECCCCCEEEHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNVGTNAADEESSAVHAQGLVIRDLPLIASNYRNEEGLSDYLKRHNIVAIADIDTRKLTR
CCCCCCCCCCCCCCHHHCCEEEECCCHHHHCCCCCCHHHHHHHHCCEEEEEECCHHHHHH
LLREKGAQNGCIIAADTPDAALALAKAKGFPGLKGMDLAKEVTTQEAYSWQQGSWTLEGE
HHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECC
LPEAKAASELPFHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTPADEVLKMAPDGIFLSN
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCEEECC
GPGDPEPCDYAITAIKQFLETDIPVFGICLGHQLLALASGAKTVKMKLGHHGGNHPVKDL
CCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHC
DKDCVMITAQNHGFAVDENNLPAELRVTHKSLFDHTVQGIHRTDKAAFSFQGHPEASPGP
CCCEEEEEECCCCEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
HDAAPLFDHFIELIETYRSNAK
CCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA