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The map label for this gene is cynT [H]

Identifier: 157368811

GI number: 157368811

Start: 629129

End: 629764

Strand: Direct

Name: cynT [H]

Synonym: Spro_0566

Alternate gene names: 157368811

Gene position: 629129-629764 (Clockwise)

Preceding gene: 157368810

Following gene: 157368812

Centisome position: 11.55

GC content: 54.4

Gene sequence:

>636_bases
ATGCAACATATCATTGAAGGTTTCCTCAGCTTCCAGAAAGAGATTTTCCCACAGCGTAAGGAACTCTTCCGCAGCCTTGC
CTCCAGCCAGAACCCTAAAGCGCTTTTTATTTCGTGTTCTGACAGCCGCCTGGTACCCGAGCTTGTGACTCAGCAAGACC
CGGGGCAGCTTTTCGTTATCCGTAATGCAGGCAACATCGTTCCTTCCTTTGGTCCGGAACCGGGCGGTGTGTCGGCGACC
ATCGAATACGCGGTGGTCGCATTGGGCGTCACGGATATCGTGATTTGCGGCCACTCGAACTGCGGCGCCATGAAGGCGAT
AGCTTCTTGCCAGTGCCTTGATCCGATGCCGGCGGTGGCCCACTGGCTGCACTATGCGGACGCGGCCAAAGCGGTGGTAG
AGAAGAAAACCTGGGATAACGAAATCGACAAAGTTAACGCTATGGTCGAAGAGAACGTTATCGCCCAGCTAAATAACATT
AAGACTCACCCGTCGGTTGCCGTGGGCCTGCGCAACAACGGCCTGCGCCTGCACGGTTGGGTGTACGACATCGAAAGTGG
CGAGATCCGCACGCTGGATAAAAATACGAAGAATTTCGTGTCACTGGCGGATAACCCCGCAGTTCACTTCGAATAA

Upstream 100 bases:

>100_bases
CTCGCAGTCCCCTGAGCGTTCATTTCCCTCCAATAGATACTGGACTAACCGGGAGTGATGATTATCATGTTATCGATACT
ACCGATCCAGAGAGCGTGTT

Downstream 100 bases:

>100_bases
AGCGATGCCTGGCCTTGGCTATGAAATTCAATCCACGTTTCGGCGTGGATTTTTTTAGGTCTAGATCTAATTAGTTGAAT
GTCATTAATCATGTTCGCTT

Product: carbonate dehydratase

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MQHIIEGFLSFQKEIFPQRKELFRSLASSQNPKALFISCSDSRLVPELVTQQDPGQLFVIRNAGNIVPSFGPEPGGVSAT
IEYAVVALGVTDIVICGHSNCGAMKAIASCQCLDPMPAVAHWLHYADAAKAVVEKKTWDNEIDKVNAMVEENVIAQLNNI
KTHPSVAVGLRNNGLRLHGWVYDIESGEIRTLDKNTKNFVSLADNPAVHFE

Sequences:

>Translated_211_residues
MQHIIEGFLSFQKEIFPQRKELFRSLASSQNPKALFISCSDSRLVPELVTQQDPGQLFVIRNAGNIVPSFGPEPGGVSAT
IEYAVVALGVTDIVICGHSNCGAMKAIASCQCLDPMPAVAHWLHYADAAKAVVEKKTWDNEIDKVNAMVEENVIAQLNNI
KTHPSVAVGLRNNGLRLHGWVYDIESGEIRTLDKNTKNFVSLADNPAVHFE
>Mature_211_residues
MQHIIEGFLSFQKEIFPQRKELFRSLASSQNPKALFISCSDSRLVPELVTQQDPGQLFVIRNAGNIVPSFGPEPGGVSAT
IEYAVVALGVTDIVICGHSNCGAMKAIASCQCLDPMPAVAHWLHYADAAKAVVEKKTWDNEIDKVNAMVEENVIAQLNNI
KTHPSVAVGLRNNGLRLHGWVYDIESGEIRTLDKNTKNFVSLADNPAVHFE

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=208, Percent_Identity=66.8269230769231, Blast_Score=299, Evalue=1e-82,
Organism=Escherichia coli, GI1786318, Length=199, Percent_Identity=30.6532663316583, Blast_Score=87, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI25147564, Length=237, Percent_Identity=29.957805907173, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI115532990, Length=238, Percent_Identity=26.890756302521, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324292, Length=195, Percent_Identity=30.2564102564103, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24645213, Length=249, Percent_Identity=24.4979919678715, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 23124; Mature: 23124

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHIIEGFLSFQKEIFPQRKELFRSLASSQNPKALFISCSDSRLVPELVTQQDPGQLFVI
CHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEE
RNAGNIVPSFGPEPGGVSATIEYAVVALGVTDIVICGHSNCGAMKAIASCQCLDPMPAVA
ECCCCCCCCCCCCCCCCCHHHHHHHEEECCEEEEEECCCCCCHHHHHHHCCCCCCHHHHH
HWLHYADAAKAVVEKKTWDNEIDKVNAMVEENVIAQLNNIKTHPSVAVGLRNNGLRLHGW
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEE
VYDIESGEIRTLDKNTKNFVSLADNPAVHFE
EEEECCCCEEEECCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MQHIIEGFLSFQKEIFPQRKELFRSLASSQNPKALFISCSDSRLVPELVTQQDPGQLFVI
CHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEE
RNAGNIVPSFGPEPGGVSATIEYAVVALGVTDIVICGHSNCGAMKAIASCQCLDPMPAVA
ECCCCCCCCCCCCCCCCCHHHHHHHEEECCEEEEEECCCCCCHHHHHHHCCCCCCHHHHH
HWLHYADAAKAVVEKKTWDNEIDKVNAMVEENVIAQLNNIKTHPSVAVGLRNNGLRLHGW
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEE
VYDIESGEIRTLDKNTKNFVSLADNPAVHFE
EEEECCCCEEEECCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]