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The map label for this gene is nusA [H]

Identifier: 157368735

GI number: 157368735

Start: 537943

End: 539451

Strand: Direct

Name: nusA [H]

Synonym: Spro_0488

Alternate gene names: 157368735

Gene position: 537943-539451 (Clockwise)

Preceding gene: 229593539

Following gene: 157368736

Centisome position: 9.87

GC content: 54.27

Gene sequence:

>1509_bases
ATGAATAAAGAGATTCTGGCTGTTGTTGAAGCTGTTTCCAATGAAAAATCCCTTCCGCGTGAGAAGATTTTCGAAGCGCT
GGAAACCGCATTAGCCACCGCCACCAAGAAAAAATACGAGCAGGAAATCGAAGTTCGCGTCAGCATTGACCGCAAAACTG
GCGATTTCGACACCTTCCGCCGTTGGGTCGCTGTAGATGAAGTGACTCAGCCAACGCGTGAAATCACGCTGGAAGCTGCA
CAGTATGAAGAGCCAGGCATCGAGCTGGGCGGTTACATCGAAGATCAGATTGAATCTGTGACCTTCGACCGTATCACCAC
TCAAACCGCGAAACAGGTTATCGTACAGAAAGTACGTGAAGCCGAGCGCGCGATGGTGGTTGACGCCTTCCGCCAGCACG
AAGGTGAAATCGTCACCGGCGTGGTGAAGAAAGTTAACCGTGACAGCATTGCGCTGGATCTGGGTAGCAACGCTGAAGCG
GTCATTGGCCGTGAAGATATGCTGCCACGCGAAAACTTCCGTCCGGGTGACCGTATCCGCGGTATCCTGTACGCCGTTCG
TCCAGAAGCCCGTGGCGCGCAGCTGTTTGTCAGCCGCTCCAGCGCCGAGATGCTGAAAGAACTGTTCCGCATCGAAGTAC
CGGAAATCGGCGAAGAAGTGATCGAAATTAAAGCGGCAGCCCGCGATCCTGGCTCCCGTGCAAAAATTGCAGTGAAAACC
AACGACAAACGCATCGACCCGGTCGGCGCTTGCGTAGGTATGCGCGGTGCGCGCGTTCAGGCCGTTTCGAGCGAGCTCGG
CGGCGAACGTATCGACATCATCCTGTGGGATGATAACCCTGCGCAGTTCGTCATCAACGCCATGGCGCCGGCAGACGTTG
CCTCAATCGTGGTTGATGAAGATAATTGCACTATGGATATCGCCGTTGAAGCCAGCAATCTGGCACAGGCGATCGGCCGT
AATGGCCAAAACGTGCGTTTGGCATCCCAGTTGTTAAAACAGCATCGTGACGATGACCGTTGGGAACTGAACGTGATGAC
GGCGGACGATCTGCAGGCCAAGCACCAGGCCGAAGCTCATGCCGCCATTGATACCTTCACCAAACATCTTGATATTGACG
AAGATTTCGCCACCGTACTGGTTGAAGAAGGCTTCTCTACTCTGGAAGAGTTGGCTTACGTGCCAATCAAAGAGCTGCTG
GAAATCGACGGTCTGGATGAAGATATGGTTGAAGCGTTGCGCGATCGCGCCAAAGCTGCATTGACCACGCTGGCCCTGGC
ACAAGAAGAAAGCCTGGGCGACCAAAAACCTGCTGACGATTTGCTCAACCTGCCGGGTCTTGAGCGCAGCATGGCCTTTA
AACTGGCCGCGCGTGGGGTTTGTACGCTGGAAGATCTTGCCGAGCAGGGTGTTGACGATCTGGCTGATATTGAAGGGCTT
AGCGATGAGCAAGCCGGCGAGCTGATTATGGCCGCACGTAATATCTGTTGGTTTGGCGACAACGCGTAA

Upstream 100 bases:

>100_bases
CACGGTTGCTGTGGAAGGGAAAGATGAAGTGTTCGCGCTGAGCAATATCCAGAAAGCGAACCTGGTACCCCACTTTTAAA
GTTTGGATGAGGCAACTAGG

Downstream 100 bases:

>100_bases
TAAACTGTAGCAGGAAGGAACAGCATGACGACAGATGTAACCGTAAAATCGCTGGCAGCAGAGATTCAGACTCCAGTTGA
TCGCCTGGTACAGCAGTTTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MNKEILAVVEAVSNEKSLPREKIFEALETALATATKKKYEQEIEVRVSIDRKTGDFDTFRRWVAVDEVTQPTREITLEAA
QYEEPGIELGGYIEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDAFRQHEGEIVTGVVKKVNRDSIALDLGSNAEA
VIGREDMLPRENFRPGDRIRGILYAVRPEARGAQLFVSRSSAEMLKELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSSELGGERIDIILWDDNPAQFVINAMAPADVASIVVDEDNCTMDIAVEASNLAQAIGR
NGQNVRLASQLLKQHRDDDRWELNVMTADDLQAKHQAEAHAAIDTFTKHLDIDEDFATVLVEEGFSTLEELAYVPIKELL
EIDGLDEDMVEALRDRAKAALTTLALAQEESLGDQKPADDLLNLPGLERSMAFKLAARGVCTLEDLAEQGVDDLADIEGL
SDEQAGELIMAARNICWFGDNA

Sequences:

>Translated_502_residues
MNKEILAVVEAVSNEKSLPREKIFEALETALATATKKKYEQEIEVRVSIDRKTGDFDTFRRWVAVDEVTQPTREITLEAA
QYEEPGIELGGYIEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDAFRQHEGEIVTGVVKKVNRDSIALDLGSNAEA
VIGREDMLPRENFRPGDRIRGILYAVRPEARGAQLFVSRSSAEMLKELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSSELGGERIDIILWDDNPAQFVINAMAPADVASIVVDEDNCTMDIAVEASNLAQAIGR
NGQNVRLASQLLKQHRDDDRWELNVMTADDLQAKHQAEAHAAIDTFTKHLDIDEDFATVLVEEGFSTLEELAYVPIKELL
EIDGLDEDMVEALRDRAKAALTTLALAQEESLGDQKPADDLLNLPGLERSMAFKLAARGVCTLEDLAEQGVDDLADIEGL
SDEQAGELIMAARNICWFGDNA
>Mature_502_residues
MNKEILAVVEAVSNEKSLPREKIFEALETALATATKKKYEQEIEVRVSIDRKTGDFDTFRRWVAVDEVTQPTREITLEAA
QYEEPGIELGGYIEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDAFRQHEGEIVTGVVKKVNRDSIALDLGSNAEA
VIGREDMLPRENFRPGDRIRGILYAVRPEARGAQLFVSRSSAEMLKELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSSELGGERIDIILWDDNPAQFVINAMAPADVASIVVDEDNCTMDIAVEASNLAQAIGR
NGQNVRLASQLLKQHRDDDRWELNVMTADDLQAKHQAEAHAAIDTFTKHLDIDEDFATVLVEEGFSTLEELAYVPIKELL
EIDGLDEDMVEALRDRAKAALTTLALAQEESLGDQKPADDLLNLPGLERSMAFKLAARGVCTLEDLAEQGVDDLADIEGL
SDEQAGELIMAARNICWFGDNA

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=502, Percent_Identity=86.8525896414343, Blast_Score=880, Evalue=0.0,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55454; Mature: 55454

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEILAVVEAVSNEKSLPREKIFEALETALATATKKKYEQEIEVRVSIDRKTGDFDTFR
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHH
RWVAVDEVTQPTREITLEAAQYEEPGIELGGYIEDQIESVTFDRITTQTAKQVIVQKVRE
HHHHHHHHCCCHHHHEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERAMVVDAFRQHEGEIVTGVVKKVNRDSIALDLGSNAEAVIGREDMLPRENFRPGDRIR
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCCCEEECHHHCCCCCCCCCCHHHC
GILYAVRPEARGAQLFVSRSSAEMLKELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKT
EEEEEECCCCCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEC
NDKRIDPVGACVGMRGARVQAVSSELGGERIDIILWDDNPAQFVINAMAPADVASIVVDE
CCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHEEEECCCCCCEEEEEEEC
DNCTMDIAVEASNLAQAIGRNGQNVRLASQLLKQHRDDDRWELNVMTADDLQAKHQAEAH
CCCEEEEEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHH
AAIDTFTKHLDIDEDFATVLVEEGFSTLEELAYVPIKELLEIDGLDEDMVEALRDRAKAA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHH
LTTLALAQEESLGDQKPADDLLNLPGLERSMAFKLAARGVCTLEDLAEQGVDDLADIEGL
HHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHCCC
SDEQAGELIMAARNICWFGDNA
CCHHHHHHHHHHCCEEEECCCC
>Mature Secondary Structure
MNKEILAVVEAVSNEKSLPREKIFEALETALATATKKKYEQEIEVRVSIDRKTGDFDTFR
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHH
RWVAVDEVTQPTREITLEAAQYEEPGIELGGYIEDQIESVTFDRITTQTAKQVIVQKVRE
HHHHHHHHCCCHHHHEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERAMVVDAFRQHEGEIVTGVVKKVNRDSIALDLGSNAEAVIGREDMLPRENFRPGDRIR
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCCCEEECHHHCCCCCCCCCCHHHC
GILYAVRPEARGAQLFVSRSSAEMLKELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKT
EEEEEECCCCCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEC
NDKRIDPVGACVGMRGARVQAVSSELGGERIDIILWDDNPAQFVINAMAPADVASIVVDE
CCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHEEEECCCCCCEEEEEEEC
DNCTMDIAVEASNLAQAIGRNGQNVRLASQLLKQHRDDDRWELNVMTADDLQAKHQAEAH
CCCEEEEEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHH
AAIDTFTKHLDIDEDFATVLVEEGFSTLEELAYVPIKELLEIDGLDEDMVEALRDRAKAA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHH
LTTLALAQEESLGDQKPADDLLNLPGLERSMAFKLAARGVCTLEDLAEQGVDDLADIEGL
HHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHCCC
SDEQAGELIMAARNICWFGDNA
CCHHHHHHHHHHCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]