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The map label for this gene is fadA

Identifier: 157368509

GI number: 157368509

Start: 289224

End: 290387

Strand: Reverse

Name: fadA

Synonym: Spro_0260

Alternate gene names: 157368509

Gene position: 290387-289224 (Counterclockwise)

Preceding gene: 157368510

Following gene: 157368505

Centisome position: 5.33

GC content: 59.36

Gene sequence:

>1164_bases
ATGGAAAACGTAGTTATTGTTGATGCCGTACGCACGCCGATGGGCCGCTCCAAGGGCGGCGCCTTCCGCCAGGTACGTGC
TGAAGATCTTTCCGCTCACCTGATGCGCGCCGTACTGAGCCGTAATCCGTCGCTGGATGCCGCCGAAATTGACGACATTT
ATTGGGGTTGCGTGCAGCAGACGCTGGAGCAGGGCTTTAACATTGCCCGTAACGCTTCGCTGCTGGCCGAGATCCCGCAC
AGTGTTCCGGCGGTCACCGTCAACCGGCTGTGCGGCTCCTCCATGCAGGCACTGCACGATGCCGCACGCGCCATTATGGT
CGGCGATGCACACGTCAGCCTGATTGGTGGCGTAGAACATATGGGCCACGTACCGATGAATCACGGTGTGGATTTCCACC
CGGGTCTGAGTCGCAGCGTGGCAAAAGCCGCCGGAATGATGGGGCTGACCGCCGAGATGCTGGCCAAGATGCACAATATC
AGCCGCCAGATGCAGGATGAGTTCGCGGCACGCTCGCACCAACGCGCACATGCTGCCACCCTGGCCGGCTATTTCAAGAA
TGAAATCATCCCAACCACCGGCCACGACGCCGACGGTGTGCTGACCCGCTATGACTTCGACGAGGTCATCCGCCCGGAAA
CCACCGTTGCCAGCCTGGCAGCGCTGCGTCCGGCCTTCGATCCGGTCAATGGTACCGTGACCGCCGGGACTTCTTCGGCA
TTGTCCGACGGTGCCTCTGCCATGCTGTTGATGAGTGAATCACGTGCCAAAGCGCTTGGCCTGAAAGCCCGTGCCCGCAT
TCGTTCAATGGCCGTTGTCGGTTGTGACCCTTCCATCATGGGTTACGGCCCTGTGCCTGCCAGCAAGTTGGCGCTCAAAC
GTGCCGGCCTGAGCGTACAGGACATCGACCTGTTTGAACTGAATGAAGCCTTTGCCGCCCAGTCACTGCCGTGCATCAAG
GATTTGGGACTGTTGGACAGCATCGACGACAAAATCAACCTGAACGGCGGCGCCATTGCTCTGGGCCACCCATTAGGCTG
CTCGGGCTCACGTATCTCGACTACCCTGCTGAACAATATGGAACGCCGCGACGTACAGTTTGGTTTGGCGACCATGTGTA
TCGGTTTGGGCCAGGGGATTGCTACCGTTTTTGAACGCGTTTAG

Upstream 100 bases:

>100_bases
GCCGGCCTGCGCGCCAAAGCTGAACATAATGAAAGCTACTACCCGGTGGCAGCGCCGCTGTCCGATGTCTCCACTGGCCA
ACCGGCATGAGGTCATAAAG

Downstream 100 bases:

>100_bases
CTGTGTCTATTAGGGATGACTGCGTCAACACTTCAAACCGCAGCATTCCACCAAGTTTAGTTGCCGTACTTCCCGCCTGT
CAGGGGCGGGTTTTTTTATG

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MENVVIVDAVRTPMGRSKGGAFRQVRAEDLSAHLMRAVLSRNPSLDAAEIDDIYWGCVQQTLEQGFNIARNASLLAEIPH
SVPAVTVNRLCGSSMQALHDAARAIMVGDAHVSLIGGVEHMGHVPMNHGVDFHPGLSRSVAKAAGMMGLTAEMLAKMHNI
SRQMQDEFAARSHQRAHAATLAGYFKNEIIPTTGHDADGVLTRYDFDEVIRPETTVASLAALRPAFDPVNGTVTAGTSSA
LSDGASAMLLMSESRAKALGLKARARIRSMAVVGCDPSIMGYGPVPASKLALKRAGLSVQDIDLFELNEAFAAQSLPCIK
DLGLLDSIDDKINLNGGAIALGHPLGCSGSRISTTLLNNMERRDVQFGLATMCIGLGQGIATVFERV

Sequences:

>Translated_387_residues
MENVVIVDAVRTPMGRSKGGAFRQVRAEDLSAHLMRAVLSRNPSLDAAEIDDIYWGCVQQTLEQGFNIARNASLLAEIPH
SVPAVTVNRLCGSSMQALHDAARAIMVGDAHVSLIGGVEHMGHVPMNHGVDFHPGLSRSVAKAAGMMGLTAEMLAKMHNI
SRQMQDEFAARSHQRAHAATLAGYFKNEIIPTTGHDADGVLTRYDFDEVIRPETTVASLAALRPAFDPVNGTVTAGTSSA
LSDGASAMLLMSESRAKALGLKARARIRSMAVVGCDPSIMGYGPVPASKLALKRAGLSVQDIDLFELNEAFAAQSLPCIK
DLGLLDSIDDKINLNGGAIALGHPLGCSGSRISTTLLNNMERRDVQFGLATMCIGLGQGIATVFERV
>Mature_387_residues
MENVVIVDAVRTPMGRSKGGAFRQVRAEDLSAHLMRAVLSRNPSLDAAEIDDIYWGCVQQTLEQGFNIARNASLLAEIPH
SVPAVTVNRLCGSSMQALHDAARAIMVGDAHVSLIGGVEHMGHVPMNHGVDFHPGLSRSVAKAAGMMGLTAEMLAKMHNI
SRQMQDEFAARSHQRAHAATLAGYFKNEIIPTTGHDADGVLTRYDFDEVIRPETTVASLAALRPAFDPVNGTVTAGTSSA
LSDGASAMLLMSESRAKALGLKARARIRSMAVVGCDPSIMGYGPVPASKLALKRAGLSVQDIDLFELNEAFAAQSLPCIK
DLGLLDSIDDKINLNGGAIALGHPLGCSGSRISTTLLNNMERRDVQFGLATMCIGLGQGIATVFERV

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=41.4572864321608, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI4501853, Length=397, Percent_Identity=42.8211586901763, Blast_Score=262, Evalue=3e-70,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=39.9497487437186, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI4557237, Length=407, Percent_Identity=34.8894348894349, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=33.7995337995338, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI194353979, Length=241, Percent_Identity=35.2697095435685, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI48994986, Length=387, Percent_Identity=84.4961240310078, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI1787663, Length=405, Percent_Identity=46.4197530864198, Blast_Score=328, Evalue=2e-91,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=41.645885286783, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788554, Length=406, Percent_Identity=44.5812807881773, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1788683, Length=416, Percent_Identity=34.375, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI133906874, Length=399, Percent_Identity=41.6040100250627, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=33.7209302325581, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17535921, Length=403, Percent_Identity=33.4987593052109, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25147385, Length=399, Percent_Identity=31.077694235589, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=395, Percent_Identity=26.8354430379747, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322031, Length=396, Percent_Identity=40.6565656565657, Blast_Score=245, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6325229, Length=405, Percent_Identity=35.5555555555556, Blast_Score=198, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=41.3965087281795, Blast_Score=267, Evalue=9e-72,
Organism=Drosophila melanogaster, GI17648125, Length=404, Percent_Identity=39.6039603960396, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=34.7319347319347, Blast_Score=204, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24640423, Length=407, Percent_Identity=32.1867321867322, Blast_Score=172, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FADA_SERP5 (A8G8D0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476498.1
- ProteinModelPortal:   A8G8D0
- SMR:   A8G8D0
- STRING:   A8G8D0
- GeneID:   5606612
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0260
- eggNOG:   COG0183
- HOGENOM:   HBG370930
- OMA:   AIDDIYW
- ProtClustDB:   PRK08947
- BioCyc:   SPRO399741:SPRO_0260-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01620
- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930
- TIGRFAMs:   TIGR02445

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: =2.3.1.16

Molecular weight: Translated: 41049; Mature: 41049

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3; PS00178 AA_TRNA_LIGASE_I

Important sites: ACT_SITE 91-91 ACT_SITE 343-343 ACT_SITE 373-373

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENVVIVDAVRTPMGRSKGGAFRQVRAEDLSAHLMRAVLSRNPSLDAAEIDDIYWGCVQQ
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TLEQGFNIARNASLLAEIPHSVPAVTVNRLCGSSMQALHDAARAIMVGDAHVSLIGGVEH
HHHHCCCHHHCCHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHEEEECCCCEEEHHCHHH
MGHVPMNHGVDFHPGLSRSVAKAAGMMGLTAEMLAKMHNISRQMQDEFAARSHQRAHAAT
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGYFKNEIIPTTGHDADGVLTRYDFDEVIRPETTVASLAALRPAFDPVNGTVTAGTSSA
HHHHHHCCCCCCCCCCCCCEEEECCHHHHHCCCHHHHHHHHHCCCCCCCCCEEECCCCHH
LSDGASAMLLMSESRAKALGLKARARIRSMAVVGCDPSIMGYGPVPASKLALKRAGLSVQ
HHCCCCEEEEEECCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCEE
DIDLFELNEAFAAQSLPCIKDLGLLDSIDDKINLNGGAIALGHPLGCSGSRISTTLLNNM
CCHHHHHHHHHHHCCCCHHHHCCCHHCCCCCEECCCCEEEECCCCCCCCCHHHHHHHHHH
ERRDVQFGLATMCIGLGQGIATVFERV
HHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MENVVIVDAVRTPMGRSKGGAFRQVRAEDLSAHLMRAVLSRNPSLDAAEIDDIYWGCVQQ
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TLEQGFNIARNASLLAEIPHSVPAVTVNRLCGSSMQALHDAARAIMVGDAHVSLIGGVEH
HHHHCCCHHHCCHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHEEEECCCCEEEHHCHHH
MGHVPMNHGVDFHPGLSRSVAKAAGMMGLTAEMLAKMHNISRQMQDEFAARSHQRAHAAT
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGYFKNEIIPTTGHDADGVLTRYDFDEVIRPETTVASLAALRPAFDPVNGTVTAGTSSA
HHHHHHCCCCCCCCCCCCCEEEECCHHHHHCCCHHHHHHHHHCCCCCCCCCEEECCCCHH
LSDGASAMLLMSESRAKALGLKARARIRSMAVVGCDPSIMGYGPVPASKLALKRAGLSVQ
HHCCCCEEEEEECCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCEE
DIDLFELNEAFAAQSLPCIKDLGLLDSIDDKINLNGGAIALGHPLGCSGSRISTTLLNNM
CCHHHHHHHHHHHCCCCHHHHCCCHHCCCCCEECCCCEEEECCCCCCCCCHHHHHHHHHH
ERRDVQFGLATMCIGLGQGIATVFERV
HHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA