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The map label for this gene is tusA

Identifier: 157368454

GI number: 157368454

Start: 230845

End: 231090

Strand: Direct

Name: tusA

Synonym: Spro_0205

Alternate gene names: 157368454

Gene position: 230845-231090 (Clockwise)

Preceding gene: 157368451

Following gene: 157368459

Centisome position: 4.24

GC content: 56.1

Gene sequence:

>246_bases
ATGACTGACTTATTTGCCCAGGCCGACCAGACGCTTGATGCGCTGGGGCTGCGTTGTCCTGAACCGGTAATGATGGTGCG
CAAAACCGTGCGCCATATGGATAACGGTGAAACTCTGCTGATCATCGCTGACGATCCGGCTACCACCCGTGACATTCCGG
GGTTCTGCCGCTTTATGGAACACACGCTGGTGGCGCAGGAAACCGACACTGCGCCTTACCGCTATTTGTTGCGCAAAGGC
GTTTGA

Upstream 100 bases:

>100_bases
AAACGTTAACGCATCTTGCACAGAAAAACTACCGGCGTAAACTATGCGCTAATGAGATGTGGCGAGCGTTCGCCGATCGT
TTTCGACCGAGAAAAATTGC

Downstream 100 bases:

>100_bases
CGTTTATCACCGCCTGTTTGCCTGGCGTTTTGTAATATTTGCTGTGCGTCAGCTGCGCGAGCATGCAATGCCAGGCCATT
GAAATCCTGTGGGCGGTAAT

Product: SirA family protein

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein A

Number of amino acids: Translated: 81; Mature: 80

Protein sequence:

>81_residues
MTDLFAQADQTLDALGLRCPEPVMMVRKTVRHMDNGETLLIIADDPATTRDIPGFCRFMEHTLVAQETDTAPYRYLLRKG
V

Sequences:

>Translated_81_residues
MTDLFAQADQTLDALGLRCPEPVMMVRKTVRHMDNGETLLIIADDPATTRDIPGFCRFMEHTLVAQETDTAPYRYLLRKG
V
>Mature_80_residues
TDLFAQADQTLDALGLRCPEPVMMVRKTVRHMDNGETLLIIADDPATTRDIPGFCRFMEHTLVAQETDTAPYRYLLRKGV

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Interacts with iscS and stimulates its activity. Then, accepts a sulfur from iscS and transfers it in turn to tusD

COG id: COG0425

COG function: function code O; Predicted redox protein, regulator of disulfide bond formation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0033 family. TusA subfamily

Homologues:

Organism=Escherichia coli, GI1789881, Length=80, Percent_Identity=83.75, Blast_Score=140, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TUSA_SERP5 (A8G875)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476443.1
- ProteinModelPortal:   A8G875
- SMR:   A8G875
- STRING:   A8G875
- GeneID:   5605775
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0205
- eggNOG:   COG0425
- HOGENOM:   HBG731190
- OMA:   HTLIASQ
- BioCyc:   SPRO399741:SPRO_0205-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00413
- InterPro:   IPR001455
- InterPro:   IPR022931
- Gene3D:   G3DSA:3.30.110.40

Pfam domain/function: PF01206 SirA; SSF64307 SirA_like

EC number: NA

Molecular weight: Translated: 9183; Mature: 9052

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01148 UPF0033

Important sites: ACT_SITE 19-19

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLFAQADQTLDALGLRCPEPVMMVRKTVRHMDNGETLLIIADDPATTRDIPGFCRFME
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHH
HTLVAQETDTAPYRYLLRKGV
HHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TDLFAQADQTLDALGLRCPEPVMMVRKTVRHMDNGETLLIIADDPATTRDIPGFCRFME
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHH
HTLVAQETDTAPYRYLLRKGV
HHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA