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The map label for this gene is eptB [H]

Identifier: 157368380

GI number: 157368380

Start: 139262

End: 140956

Strand: Direct

Name: eptB [H]

Synonym: Spro_0131

Alternate gene names: 157368380

Gene position: 139262-140956 (Clockwise)

Preceding gene: 157368379

Following gene: 157368381

Centisome position: 2.56

GC content: 52.74

Gene sequence:

>1695_bases
ATGAACAAAGTTAAATCGCTATCACAGCAGAATTTATCGTTATTGTTAGCGATCTATATCGGCATCTTTTTGAACCTTTC
CGTTTTCTATCGTCGCTTTGATTCGTTTTCGCACGGCATTCAGGGGATTAAGGTCATTTCTGCGGTAACCGAGGTTATCG
CCATCGTTCTGTTCACCTTCTTTATTATGCGGCTGGTCTCGCTCGGCGGCCGGCTGTTCTACCGGATTATTGCCTCGCTA
TTGGTGCTGATTTCCGTGGCCGCCAGCTATTACATGACGTTTTTCAACGTGGTGATCGGCTACGGCATTGTGGTGTCGGT
GATGACCACCGATATCGACCTGTCGAAAGAAGTGGTCGGTATGCACTTCGTGCTGTGGATGGTGGTGCTCAGCGCATTGC
CGCTGCTGTGCATCTGGAAAAACAACCTGCGTTATACCCTGATCGAGCAACTGAAAACGCCCGGACACCGGATAAAACCG
CTGGTGGTGCTGGTGGCGGTGGTGGCGCTGGTCTGGGTGCCGCTGCGTATGCTGGATAACGAGCAAAGCGCGCAGGAAAA
AATCACTAATGTGGATTTGCCAAGCTACGGTGGCGTGGTTGCACATTCCTATTTGCCATCCAACTGGCTGTCGGCGTTGG
GTCTGTTTGCCTACACCCGTTATGACGAAAGTCAGGATCAGGCCAATTTGTTCGACCCGGCCAAGAATTTTACCTATGTT
GCACCGGCCGATATTGATGATACCTACGTGGTGTTTATCATTGGTGAAACCACGCGCTGGGATCACATGGGCATTCTTGG
CTACGACCGTGACACTACGCCGCGGCTATCCAAAGAGAAAAATCTGGTGGCGTTCCGCGGCACGTCTTGTGATACCGCCA
CCAAACTGTCGTTGCGCTGTATGTTCGTGCGGGAAGGCGGCACGGCAGATAACCCGCAACGGACTCTGAAAGAGCAAAAC
GTGTTCGCGGTAATGAAAGATTTGGGCTTCACCTCCGAGCTGTTCGCCATGCAGAGTGAGGTTTGGTTCTATAACAACAC
CGAAGTGAACAACTATTCGTTCCGTGAGATGATCGCCTCCGAAAAGCGCAACGACGGCAAATCGGTTGACGATATGCTGT
TGGTGGATGAAATGAAGGAGTCGCTGGCCCGTTATCCTAAGGGCAAGCATCTGGTGATCCTGCACACCAAGGGCTCACAT
TATCTCTATTCCCAGCGTTATCCGCGTAGCTACGCGCGTTATCAGCCGGAGTGTATCGGCGTTGACGATTTCTGCTCCAA
GGACCAGTTGGTTAACGCCTTCGATAACTCGGTGCTGTATACCGACAGCTTTATCGCCAATGTGATTGATCAGGTGCGCG
AGAAGAAGGCGATTGTGTTCTATTCTTCCGATCACGGCGAATCGATCGGCGAGAATTCACACCTGCACGGCACGCCGCGT
GAAATGGCGCCGGTGGAGCAGTTCCGCGTGCCAATGATGGTATGGGCATCCGACAAGTTCCTGGAAGACCCACAGCATCG
CGGAGCGTTTGAGCAATTGCAGGCGCAGCAGCGGGTTGGCAAGACCCACCGCCATGTTGAGCTGTTCGATACCATTCTCG
GCTGCCTCGGTTATACCTCGCCGAACGGCGGCATCGTGGCCAAAAACAACTGGTGTGATATTCCGCAGGAAAAAGCGTCG
CCAAGCGAGCTGTAA

Upstream 100 bases:

>100_bases
CCCCCAAAATAGCGCTTATTGCCGATCGGCTGAACCTGCAGCCGCATCGAATGTCTGACTGACTTCAGAACTGTTAAAGC
CCCCGGAGCCACCGCGTTAC

Downstream 100 bases:

>100_bases
CCTCTCCAATACCGGGCGCACTGCGCCCGGTAAAATCTGCTTATACCTTCCTTTCATCAACTAATCTGGCTCAGGCTTGC
GCTATACTTGCGCACTTTCC

Product: phosphoethanolamine transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MNKVKSLSQQNLSLLLAIYIGIFLNLSVFYRRFDSFSHGIQGIKVISAVTEVIAIVLFTFFIMRLVSLGGRLFYRIIASL
LVLISVAASYYMTFFNVVIGYGIVVSVMTTDIDLSKEVVGMHFVLWMVVLSALPLLCIWKNNLRYTLIEQLKTPGHRIKP
LVVLVAVVALVWVPLRMLDNEQSAQEKITNVDLPSYGGVVAHSYLPSNWLSALGLFAYTRYDESQDQANLFDPAKNFTYV
APADIDDTYVVFIIGETTRWDHMGILGYDRDTTPRLSKEKNLVAFRGTSCDTATKLSLRCMFVREGGTADNPQRTLKEQN
VFAVMKDLGFTSELFAMQSEVWFYNNTEVNNYSFREMIASEKRNDGKSVDDMLLVDEMKESLARYPKGKHLVILHTKGSH
YLYSQRYPRSYARYQPECIGVDDFCSKDQLVNAFDNSVLYTDSFIANVIDQVREKKAIVFYSSDHGESIGENSHLHGTPR
EMAPVEQFRVPMMVWASDKFLEDPQHRGAFEQLQAQQRVGKTHRHVELFDTILGCLGYTSPNGGIVAKNNWCDIPQEKAS
PSEL

Sequences:

>Translated_564_residues
MNKVKSLSQQNLSLLLAIYIGIFLNLSVFYRRFDSFSHGIQGIKVISAVTEVIAIVLFTFFIMRLVSLGGRLFYRIIASL
LVLISVAASYYMTFFNVVIGYGIVVSVMTTDIDLSKEVVGMHFVLWMVVLSALPLLCIWKNNLRYTLIEQLKTPGHRIKP
LVVLVAVVALVWVPLRMLDNEQSAQEKITNVDLPSYGGVVAHSYLPSNWLSALGLFAYTRYDESQDQANLFDPAKNFTYV
APADIDDTYVVFIIGETTRWDHMGILGYDRDTTPRLSKEKNLVAFRGTSCDTATKLSLRCMFVREGGTADNPQRTLKEQN
VFAVMKDLGFTSELFAMQSEVWFYNNTEVNNYSFREMIASEKRNDGKSVDDMLLVDEMKESLARYPKGKHLVILHTKGSH
YLYSQRYPRSYARYQPECIGVDDFCSKDQLVNAFDNSVLYTDSFIANVIDQVREKKAIVFYSSDHGESIGENSHLHGTPR
EMAPVEQFRVPMMVWASDKFLEDPQHRGAFEQLQAQQRVGKTHRHVELFDTILGCLGYTSPNGGIVAKNNWCDIPQEKAS
PSEL
>Mature_564_residues
MNKVKSLSQQNLSLLLAIYIGIFLNLSVFYRRFDSFSHGIQGIKVISAVTEVIAIVLFTFFIMRLVSLGGRLFYRIIASL
LVLISVAASYYMTFFNVVIGYGIVVSVMTTDIDLSKEVVGMHFVLWMVVLSALPLLCIWKNNLRYTLIEQLKTPGHRIKP
LVVLVAVVALVWVPLRMLDNEQSAQEKITNVDLPSYGGVVAHSYLPSNWLSALGLFAYTRYDESQDQANLFDPAKNFTYV
APADIDDTYVVFIIGETTRWDHMGILGYDRDTTPRLSKEKNLVAFRGTSCDTATKLSLRCMFVREGGTADNPQRTLKEQN
VFAVMKDLGFTSELFAMQSEVWFYNNTEVNNYSFREMIASEKRNDGKSVDDMLLVDEMKESLARYPKGKHLVILHTKGSH
YLYSQRYPRSYARYQPECIGVDDFCSKDQLVNAFDNSVLYTDSFIANVIDQVREKKAIVFYSSDHGESIGENSHLHGTPR
EMAPVEQFRVPMMVWASDKFLEDPQHRGAFEQLQAQQRVGKTHRHVELFDTILGCLGYTSPNGGIVAKNNWCDIPQEKAS
PSEL

Specific function: Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (KDO) residue of a KDO(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases dia

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082286, Length=562, Percent_Identity=66.7259786476868, Blast_Score=800, Evalue=0.0,
Organism=Escherichia coli, GI87082372, Length=312, Percent_Identity=32.0512820512821, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI87082223, Length=461, Percent_Identity=24.5119305856833, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1790392, Length=491, Percent_Identity=23.2179226069246, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 63991; Mature: 63991

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 9298646 [H]