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The map label for this gene is xylF [H]

Identifier: 157368348

GI number: 157368348

Start: 106406

End: 107401

Strand: Direct

Name: xylF [H]

Synonym: Spro_0099

Alternate gene names: 157368348

Gene position: 106406-107401 (Clockwise)

Preceding gene: 157368342

Following gene: 157368349

Centisome position: 1.95

GC content: 54.82

Gene sequence:

>996_bases
ATGAAATTCAAACCTGTGTTCCTTTCGGTCTGTGCGCTGCTGGCGCTGGCGAGCCAACCGGCGCAGAGCAAAGAAGTCAA
AATCGGCATGGCGATCGACGACCTGCGCCTGGAGCGCTGGCAGAAAGACCGCGACATCTTCGTCAGCAAAGCCAAAGCGC
TGGGGGCGGATGTGTTTGTTCAGTCGGCCAATGGCAATGAAGAAACCCAGATGGCGCAGATTGAAAATATGATCAACCGC
GGCGTCGACGTGCTGGTGATTATTCCCTACAACGGCAAGGTGTTGAGTAACGTCATCAGCGAAGCCAAGCGGGAAGGCAT
TAAGGTGTTGGCTTACGATCGCATGATCAATAACGCCGATATCGATTTTTACATCTCCTTTGATAATGAAAAGGTCGGTG
AGCTGCAGGCGCAAAGCCTGGTGCAGCGGGTGCCGCAGGGCAATTATTTCCTGATGGGCGGCTCGCCGGTGGATAACAAC
GCCAAGCTGTTCCGCCAGGGACAGATGAAGGTGCTGCAACCGCTGATTGACGGCGGGAAAATCAAAGTGGTGGGCGATCA
GTGGGTCGACGGATGGTTGCCGGAAAACGCATTAAAAATCATGGAAAATGCGCTGACCGCCAACAATAACAAGATTGACG
CGGTAGTGGCTTCCAACGATGCCACCGCCGGCGGCGCTATCCAGGCGCTGGCCGCGCAGGGCCTGGCGGGCAAGGTGGCT
ATTTCCGGTCAGGATGCCGATTTGGCGGCGGTGAAGCGCATTGTCGCCGGTACCCAGACCATGACGGTGTATAAGCCAAT
CAGCAAACTGGCCGACGAGGCGGCGGGCATCGCGGTGCAACTGGGCAAGGGTGAACAGCCGAAGGCTAACGCCACATTAA
ATAACGGCAGCAAAGACGTGCCATCCTGGTTATTAACCCCTATTCCGGTTGATAAATCGAATATCGACAGCACCATTGTT
GCCGACGGTTTCCATAAAAAAGCCGAGATTAATTAA

Upstream 100 bases:

>100_bases
ATTGCTGAATATTAAATAACCGATAACAATCCGCTCTAAGTTGCATCACCAGTCTGTTTCAATTATTGCCCCTACCCGTT
AAATAACAAAGGTATATAAG

Downstream 100 bases:

>100_bases
TTCCGCAAGCCGGTAATCCGGAGGGAATAATGCCCAGCCTATTAGAAATGAAAAATATCACCATGCAATTCGGTGCGGTC
AAGGCGGTAGATAATGTCAG

Product: D-xylose transporter subunit XylF

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKFKPVFLSVCALLALASQPAQSKEVKIGMAIDDLRLERWQKDRDIFVSKAKALGADVFVQSANGNEETQMAQIENMINR
GVDVLVIIPYNGKVLSNVISEAKREGIKVLAYDRMINNADIDFYISFDNEKVGELQAQSLVQRVPQGNYFLMGGSPVDNN
AKLFRQGQMKVLQPLIDGGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATAGGAIQALAAQGLAGKVA
ISGQDADLAAVKRIVAGTQTMTVYKPISKLADEAAGIAVQLGKGEQPKANATLNNGSKDVPSWLLTPIPVDKSNIDSTIV
ADGFHKKAEIN

Sequences:

>Translated_331_residues
MKFKPVFLSVCALLALASQPAQSKEVKIGMAIDDLRLERWQKDRDIFVSKAKALGADVFVQSANGNEETQMAQIENMINR
GVDVLVIIPYNGKVLSNVISEAKREGIKVLAYDRMINNADIDFYISFDNEKVGELQAQSLVQRVPQGNYFLMGGSPVDNN
AKLFRQGQMKVLQPLIDGGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATAGGAIQALAAQGLAGKVA
ISGQDADLAAVKRIVAGTQTMTVYKPISKLADEAAGIAVQLGKGEQPKANATLNNGSKDVPSWLLTPIPVDKSNIDSTIV
ADGFHKKAEIN
>Mature_331_residues
MKFKPVFLSVCALLALASQPAQSKEVKIGMAIDDLRLERWQKDRDIFVSKAKALGADVFVQSANGNEETQMAQIENMINR
GVDVLVIIPYNGKVLSNVISEAKREGIKVLAYDRMINNADIDFYISFDNEKVGELQAQSLVQRVPQGNYFLMGGSPVDNN
AKLFRQGQMKVLQPLIDGGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATAGGAIQALAAQGLAGKVA
ISGQDADLAAVKRIVAGTQTMTVYKPISKLADEAAGIAVQLGKGEQPKANATLNNGSKDVPSWLLTPIPVDKSNIDSTIV
ADGFHKKAEIN

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=330, Percent_Identity=80.9090909090909, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1790526, Length=225, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35573; Mature: 35573

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKPVFLSVCALLALASQPAQSKEVKIGMAIDDLRLERWQKDRDIFVSKAKALGADVFV
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHCCCEEEE
QSANGNEETQMAQIENMINRGVDVLVIIPYNGKVLSNVISEAKREGIKVLAYDRMINNAD
ECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
IDFYISFDNEKVGELQAQSLVQRVPQGNYFLMGGSPVDNNAKLFRQGQMKVLQPLIDGGK
EEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHCHHHHHHHHHCCCE
IKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATAGGAIQALAAQGLAGKVA
EEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCEEE
ISGQDADLAAVKRIVAGTQTMTVYKPISKLADEAAGIAVQLGKGEQPKANATLNNGSKDV
ECCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCEEEEECCCCCCCCCCEECCCCCCC
PSWLLTPIPVDKSNIDSTIVADGFHKKAEIN
CHHHCCCCCCCCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MKFKPVFLSVCALLALASQPAQSKEVKIGMAIDDLRLERWQKDRDIFVSKAKALGADVFV
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHCCCEEEE
QSANGNEETQMAQIENMINRGVDVLVIIPYNGKVLSNVISEAKREGIKVLAYDRMINNAD
ECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
IDFYISFDNEKVGELQAQSLVQRVPQGNYFLMGGSPVDNNAKLFRQGQMKVLQPLIDGGK
EEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHCHHHHHHHHHCCCE
IKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATAGGAIQALAAQGLAGKVA
EEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCEEE
ISGQDADLAAVKRIVAGTQTMTVYKPISKLADEAAGIAVQLGKGEQPKANATLNNGSKDV
ECCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCEEEEECCCCCCCCCCEECCCCCCC
PSWLLTPIPVDKSNIDSTIVADGFHKKAEIN
CHHHCCCCCCCCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]