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The map label for this gene is selB [H]

Identifier: 157368340

GI number: 157368340

Start: 95415

End: 97262

Strand: Direct

Name: selB [H]

Synonym: Spro_0091

Alternate gene names: 157368340

Gene position: 95415-97262 (Clockwise)

Preceding gene: 157368339

Following gene: 157368342

Centisome position: 1.75

GC content: 62.18

Gene sequence:

>1848_bases
ATGATTATCGCTACCGCCGGTCATGTCGATCATGGCAAAACCACCCTGCTGCAGGCGATCACCGGGATTAACGCCGATCG
CCTGCCGGAGGAGAAACGCCGCGGCATGACCCTGGATCTGGGCTATGCCTACTGGCCGCAGCCCGATGGCCGGGTGCTGG
GCTTTATCGACGTGCCCGGTCATGAAAAATTCCTCGCCAATATGCTGGCAGGCGTCGGCGGCATTGACCATGCGCTGCTG
GTGGTGGCCTGCGACGACGGCGTGATGGCGCAAACCCGCGAGCACCTGGCGATACTGCGGTTGAGCGGCCAGCCAGCGCT
GACGGTGGCGCTGACCAAGGCCGATCGGGTGGATGAAGCCCGCATTGAAGAAGTACGACGTCAGCTAAGTGACGAACTGG
CGCACCAGGGTTGGCATCAGGTGCCCATGTTCGTCACTGCGACGCTACAGGCGCGGGGAATCGACGCGCTGCGCGCGCAT
CTGCTGGCGTTGCAGCCTGACGAACATGCGTTAACCCGTCGCTTCCGTCTGGCGGTGGATCGCGCGTTCAGCGTTAAAGG
CGCGGGTCTGGTGGTGACCGGTACCGCATTGGGTGGGCAAGTCGCCGTGGGTGATCAGCTGTGGCTGACCGGTGCCGATA
CCCCGGTGCGGGTACGAGGCCTGCACGCGCAGAACCAACCGGTTGAGCAGGCGCAGGCCGGGCAGCGTATCGCACTGAAT
ATCAGTGGTGACGTCAGTAAAGAACAGGTGGCGCGCGGTGACTGGCTGCTGGCGCAAAAGCCGCTGGCCGCTGCCGAGCG
CATTTTGGTCGCACTGGATGCTGACCAACCGATAAAACACTGGCAACCGCTGCATCTGCATCATGCCGCCAGCCATATCA
CCGGGCGAGTTTCGCTGCTCAATGAGGGGCTGGCAGAGCTGATCCTCGATCGTCCACTCTGGTTGGCGGAAAACGATCGG
CTGGTGCTACGTGATATTGGCGCTCGCCAAACGCTGGGTGGCGCCAGAGTGCTGAGTTTGGCCACCCCGAAGCGGGGCAA
GCGTCAACCTGAGTATCTGGCCTGGCTGGCGGCGCTGGCAAAGGCCGACGATGACCGGCAGGTGCTGGCGTTGCATCTGC
CTAATGGCGCACTGGATCTGGCCGCCTTTGCCTGGGCCCGGCAACTGACGGCAGGCGAACTGGCAGCATTGCTGGTGGGC
CGTGAGTTGCTGATTGCCGGCGATATGGCGCTGGCGCAAGAGAATGTGCAGCAGGGCCAACAGCGTCTGCTACAGGTATT
GGCCGACTACCACCAACAGCATAGCGATCAGCTCGGTCTGGGGCGGGCACGCCTACGGCGCATGGCTTTGCCTCAGCTTG
CTGAACCGCTGGTGTTTATGATGATCGATCGGCTGCTGGCCGAGGGATTGATAAACAATACCCGGGGTTGGTTGCACCTG
CCGGAACACGGCCTGGCGTTCAGTGCTGAAGAACTGGCCCTGTGGGAAATTATCGAGCCGCGGTTCGGCGATGAAGCCTG
GTGGGTTCGCGATTTGGCGACCGAACTGGGCGAAGACGAGGCCCGGATACGGGCGACATTGCGTAAAGCGGCACAGTTGG
GGCATGTCACGGCGGTAGTGGTGGATCGTTACTACTTAAGCCAACGCATTGAACAGTTCGCTGCACTGATCCGTGAGCTT
GATGTGGAGCAGGGCAGCACCAATGCCGCCGATTTTCGTGACCGGCTCGGCGTGGGTCGCAAGCTGGCGATCCAGGTGCT
GGAGTTCTTCGACCGCAGCGGCTTCACCCGCCGCAAGGGCAACCAGCACCTGCTGCGCGACAGTGGGTTGTATACCCGTC
ATACTTGA

Upstream 100 bases:

>100_bases
CTCTGCCGCAGCCGGTGATTGGCCGCATTAACGACGGCAGGCTGTGGCTGGATCTACGCTGTCTGGAGCAGGAAGACGCA
TTGATTGAGGCGCTGAGCGC

Downstream 100 bases:

>100_bases
AGCTGCCTCTGTGTTGGCTGCGAGCACTCACCCCAGTCACTTACTTGAGTAAGCTCCTGGGGACTCATGCTCTTGCCGCC
TTGATGCAACTCCAATTATT

Product: selenocysteinyl-tRNA-specific translation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MIIATAGHVDHGKTTLLQAITGINADRLPEEKRRGMTLDLGYAYWPQPDGRVLGFIDVPGHEKFLANMLAGVGGIDHALL
VVACDDGVMAQTREHLAILRLSGQPALTVALTKADRVDEARIEEVRRQLSDELAHQGWHQVPMFVTATLQARGIDALRAH
LLALQPDEHALTRRFRLAVDRAFSVKGAGLVVTGTALGGQVAVGDQLWLTGADTPVRVRGLHAQNQPVEQAQAGQRIALN
ISGDVSKEQVARGDWLLAQKPLAAAERILVALDADQPIKHWQPLHLHHAASHITGRVSLLNEGLAELILDRPLWLAENDR
LVLRDIGARQTLGGARVLSLATPKRGKRQPEYLAWLAALAKADDDRQVLALHLPNGALDLAAFAWARQLTAGELAALLVG
RELLIAGDMALAQENVQQGQQRLLQVLADYHQQHSDQLGLGRARLRRMALPQLAEPLVFMMIDRLLAEGLINNTRGWLHL
PEHGLAFSAEELALWEIIEPRFGDEAWWVRDLATELGEDEARIRATLRKAAQLGHVTAVVVDRYYLSQRIEQFAALIREL
DVEQGSTNAADFRDRLGVGRKLAIQVLEFFDRSGFTRRKGNQHLLRDSGLYTRHT

Sequences:

>Translated_615_residues
MIIATAGHVDHGKTTLLQAITGINADRLPEEKRRGMTLDLGYAYWPQPDGRVLGFIDVPGHEKFLANMLAGVGGIDHALL
VVACDDGVMAQTREHLAILRLSGQPALTVALTKADRVDEARIEEVRRQLSDELAHQGWHQVPMFVTATLQARGIDALRAH
LLALQPDEHALTRRFRLAVDRAFSVKGAGLVVTGTALGGQVAVGDQLWLTGADTPVRVRGLHAQNQPVEQAQAGQRIALN
ISGDVSKEQVARGDWLLAQKPLAAAERILVALDADQPIKHWQPLHLHHAASHITGRVSLLNEGLAELILDRPLWLAENDR
LVLRDIGARQTLGGARVLSLATPKRGKRQPEYLAWLAALAKADDDRQVLALHLPNGALDLAAFAWARQLTAGELAALLVG
RELLIAGDMALAQENVQQGQQRLLQVLADYHQQHSDQLGLGRARLRRMALPQLAEPLVFMMIDRLLAEGLINNTRGWLHL
PEHGLAFSAEELALWEIIEPRFGDEAWWVRDLATELGEDEARIRATLRKAAQLGHVTAVVVDRYYLSQRIEQFAALIREL
DVEQGSTNAADFRDRLGVGRKLAIQVLEFFDRSGFTRRKGNQHLLRDSGLYTRHT
>Mature_615_residues
MIIATAGHVDHGKTTLLQAITGINADRLPEEKRRGMTLDLGYAYWPQPDGRVLGFIDVPGHEKFLANMLAGVGGIDHALL
VVACDDGVMAQTREHLAILRLSGQPALTVALTKADRVDEARIEEVRRQLSDELAHQGWHQVPMFVTATLQARGIDALRAH
LLALQPDEHALTRRFRLAVDRAFSVKGAGLVVTGTALGGQVAVGDQLWLTGADTPVRVRGLHAQNQPVEQAQAGQRIALN
ISGDVSKEQVARGDWLLAQKPLAAAERILVALDADQPIKHWQPLHLHHAASHITGRVSLLNEGLAELILDRPLWLAENDR
LVLRDIGARQTLGGARVLSLATPKRGKRQPEYLAWLAALAKADDDRQVLALHLPNGALDLAAFAWARQLTAGELAALLVG
RELLIAGDMALAQENVQQGQQRLLQVLADYHQQHSDQLGLGRARLRRMALPQLAEPLVFMMIDRLLAEGLINNTRGWLHL
PEHGLAFSAEELALWEIIEPRFGDEAWWVRDLATELGEDEARIRATLRKAAQLGHVTAVVVDRYYLSQRIEQFAALIREL
DVEQGSTNAADFRDRLGVGRKLAIQVLEFFDRSGFTRRKGNQHLLRDSGLYTRHT

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=381, Percent_Identity=29.9212598425197, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI5729864, Length=374, Percent_Identity=26.4705882352941, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI223555963, Length=374, Percent_Identity=26.4705882352941, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI54607086, Length=287, Percent_Identity=24.7386759581882, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI53729339, Length=223, Percent_Identity=32.7354260089686, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI53729337, Length=223, Percent_Identity=32.7354260089686, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI82546879, Length=324, Percent_Identity=23.4567901234568, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI45593140, Length=323, Percent_Identity=24.4582043343653, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI4503475, Length=398, Percent_Identity=22.8643216080402, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4503471, Length=397, Percent_Identity=22.9219143576826, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI2367247, Length=611, Percent_Identity=65.7937806873977, Blast_Score=809, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=385, Percent_Identity=29.6103896103896, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1789737, Length=333, Percent_Identity=30.3303303303303, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789108, Length=335, Percent_Identity=26.5671641791045, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789559, Length=95, Percent_Identity=37.8947368421053, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=293, Percent_Identity=32.0819112627986, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25141371, Length=297, Percent_Identity=29.2929292929293, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17506081, Length=293, Percent_Identity=25.5972696245734, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI115532067, Length=406, Percent_Identity=25.3694581280788, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115532065, Length=406, Percent_Identity=25.3694581280788, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17552884, Length=431, Percent_Identity=24.1299303944316, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17569207, Length=431, Percent_Identity=24.1299303944316, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71994658, Length=239, Percent_Identity=30.9623430962343, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565438, Length=333, Percent_Identity=23.1231231231231, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=304, Percent_Identity=30.9210526315789, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325337, Length=316, Percent_Identity=24.6835443037975, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319594, Length=316, Percent_Identity=24.6835443037975, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324550, Length=134, Percent_Identity=32.8358208955224, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281363316, Length=385, Percent_Identity=28.8311688311688, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17864358, Length=385, Percent_Identity=28.8311688311688, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19921738, Length=284, Percent_Identity=32.3943661971831, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922690, Length=259, Percent_Identity=33.5907335907336, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24652838, Length=407, Percent_Identity=24.0786240786241, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17137572, Length=407, Percent_Identity=24.0786240786241, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45550900, Length=329, Percent_Identity=22.7963525835866, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI45553807, Length=376, Percent_Identity=25.2659574468085, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45553816, Length=376, Percent_Identity=25.2659574468085, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24651721, Length=376, Percent_Identity=25.2659574468085, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17864154, Length=376, Percent_Identity=25.2659574468085, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28572034, Length=129, Percent_Identity=41.0852713178295, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922512, Length=331, Percent_Identity=24.4712990936556, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24654852, Length=331, Percent_Identity=24.4712990936556, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 67899; Mature: 67899

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIATAGHVDHGKTTLLQAITGINADRLPEEKRRGMTLDLGYAYWPQPDGRVLGFIDVPG
CEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECEEECCCCCCCEEEEEECCC
HEKFLANMLAGVGGIDHALLVVACDDGVMAQTREHLAILRLSGQPALTVALTKADRVDEA
HHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCEEEEEECCCCEEEEEEECCCCCCHH
RIEEVRRQLSDELAHQGWHQVPMFVTATLQARGIDALRAHLLALQPDEHALTRRFRLAVD
HHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
RAFSVKGAGLVVTGTALGGQVAVGDQLWLTGADTPVRVRGLHAQNQPVEQAQAGQRIALN
HHHEECCCCEEEEECCCCCEEEECCEEEEECCCCCEEEEECCCCCCCHHHHHCCCEEEEE
ISGDVSKEQVARGDWLLAQKPLAAAERILVALDADQPIKHWQPLHLHHAASHITGRVSLL
ECCCCCHHHHHCCCEEEECCCHHHHCEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHH
NEGLAELILDRPLWLAENDRLVLRDIGARQTLGGARVLSLATPKRGKRQPEYLAWLAALA
HHHHHHHHHCCCCEEECCCCEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
KADDDRQVLALHLPNGALDLAAFAWARQLTAGELAALLVGRELLIAGDMALAQENVQQGQ
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHEEECHHHHHHHHHHHHH
QRLLQVLADYHQQHSDQLGLGRARLRRMALPQLAEPLVFMMIDRLLAEGLINNTRGWLHL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
PEHGLAFSAEELALWEIIEPRFGDEAWWVRDLATELGEDEARIRATLRKAAQLGHVTAVV
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHH
VDRYYLSQRIEQFAALIRELDVEQGSTNAADFRDRLGVGRKLAIQVLEFFDRSGFTRRKG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
NQHLLRDSGLYTRHT
CCHHHHCCCCCCCCC
>Mature Secondary Structure
MIIATAGHVDHGKTTLLQAITGINADRLPEEKRRGMTLDLGYAYWPQPDGRVLGFIDVPG
CEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECEEECCCCCCCEEEEEECCC
HEKFLANMLAGVGGIDHALLVVACDDGVMAQTREHLAILRLSGQPALTVALTKADRVDEA
HHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCEEEEEECCCCEEEEEEECCCCCCHH
RIEEVRRQLSDELAHQGWHQVPMFVTATLQARGIDALRAHLLALQPDEHALTRRFRLAVD
HHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
RAFSVKGAGLVVTGTALGGQVAVGDQLWLTGADTPVRVRGLHAQNQPVEQAQAGQRIALN
HHHEECCCCEEEEECCCCCEEEECCEEEEECCCCCEEEEECCCCCCCHHHHHCCCEEEEE
ISGDVSKEQVARGDWLLAQKPLAAAERILVALDADQPIKHWQPLHLHHAASHITGRVSLL
ECCCCCHHHHHCCCEEEECCCHHHHCEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHH
NEGLAELILDRPLWLAENDRLVLRDIGARQTLGGARVLSLATPKRGKRQPEYLAWLAALA
HHHHHHHHHCCCCEEECCCCEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
KADDDRQVLALHLPNGALDLAAFAWARQLTAGELAALLVGRELLIAGDMALAQENVQQGQ
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHEEECHHHHHHHHHHHHH
QRLLQVLADYHQQHSDQLGLGRARLRRMALPQLAEPLVFMMIDRLLAEGLINNTRGWLHL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
PEHGLAFSAEELALWEIIEPRFGDEAWWVRDLATELGEDEARIRATLRKAAQLGHVTAVV
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHH
VDRYYLSQRIEQFAALIRELDVEQGSTNAADFRDRLGVGRKLAIQVLEFFDRSGFTRRKG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
NQHLLRDSGLYTRHT
CCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2531290; 8041620; 9278503; 2140572 [H]