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The map label for this gene is fdoI [H]

Identifier: 157368335

GI number: 157368335

Start: 90969

End: 91604

Strand: Direct

Name: fdoI [H]

Synonym: Spro_0086

Alternate gene names: 157368335

Gene position: 90969-91604 (Clockwise)

Preceding gene: 157368334

Following gene: 157368336

Centisome position: 1.67

GC content: 54.87

Gene sequence:

>636_bases
ATGAAGAAGGAAAAGCGTATTCAGCGCTACAGCGCGCCGGAGCGGATTAACCACTGGATTGTGGCGTTCTGCTTTGTGTT
CGCCGCCATTAGCGGGCTGGGGTTCTTTTTCCCCTCCTTCAACTGGTTGATGAACATTCTCGGCACGCCGCAGTTGGCGC
GTATTCTGCACCCCTTCGTTGGGGTGATTATGTTTGCGGCCTTCCTGCTGATGTTCCTGCGCTACTGGAAGCATAACCTG
ATTAACCGCGAAGACATCGTCTGGGCCAAAAACATCCAAAAAATCGTCATGAACGAGGAAGTGGGTGACACCGGGCGTTA
TAATTTCGGTCAGAAGTGCGTATTCTGGGCGGCAATCATTAGTCTGGTGCTGTTGCTGGCCAGCGGTGTGGTGATCTGGC
GGCCGTACTTTGCGCCGTCCTTCCCGATACCGCTGATCCGTATCGCGTTGCTGGTGCATTCGCTGGCGGCGGTGGGGCTT
ATCATTGTGATTATGGTGCATATTTACGCCGCGTTGTGGGTAAAAGGCACCATTACCGCGATGGTGGAAGGCTGGGTGCC
GGCGGCCTGGGCTAAAAAACATCACCCGCGTTGGTACCGAGAGGTCCGCGAGAAACAACAGGAAGACAAACCCTGA

Upstream 100 bases:

>100_bases
CCGCCACCTTTGCAGCGAGCGTGTTCCACTATGTCGGCGTCGGTCCTAACCGCGTCGAAGAGGAAGACCACGACGAGCTG
CACGATGAGGAGACGCGCAA

Downstream 100 bases:

>100_bases
TGAGCATCCGCATTGTTCCTAAAGAGCAGTTAGGGGCACAGCGTGAGAAGTCCACAACGGCGGAAACCATCCCGCCGTTA
CTTTTCGCGAATCTGAAAAG

Product: formate dehydrogenase-O subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MKKEKRIQRYSAPERINHWIVAFCFVFAAISGLGFFFPSFNWLMNILGTPQLARILHPFVGVIMFAAFLLMFLRYWKHNL
INREDIVWAKNIQKIVMNEEVGDTGRYNFGQKCVFWAAIISLVLLLASGVVIWRPYFAPSFPIPLIRIALLVHSLAAVGL
IIVIMVHIYAALWVKGTITAMVEGWVPAAWAKKHHPRWYREVREKQQEDKP

Sequences:

>Translated_211_residues
MKKEKRIQRYSAPERINHWIVAFCFVFAAISGLGFFFPSFNWLMNILGTPQLARILHPFVGVIMFAAFLLMFLRYWKHNL
INREDIVWAKNIQKIVMNEEVGDTGRYNFGQKCVFWAAIISLVLLLASGVVIWRPYFAPSFPIPLIRIALLVHSLAAVGL
IIVIMVHIYAALWVKGTITAMVEGWVPAAWAKKHHPRWYREVREKQQEDKP
>Mature_211_residues
MKKEKRIQRYSAPERINHWIVAFCFVFAAISGLGFFFPSFNWLMNILGTPQLARILHPFVGVIMFAAFLLMFLRYWKHNL
INREDIVWAKNIQKIVMNEEVGDTGRYNFGQKCVFWAAIISLVLLLASGVVIWRPYFAPSFPIPLIRIALLVHSLAAVGL
IIVIMVHIYAALWVKGTITAMVEGWVPAAWAKKHHPRWYREVREKQQEDKP

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=209, Percent_Identity=79.4258373205742, Blast_Score=359, Evalue=1e-101,
Organism=Escherichia coli, GI1787750, Length=212, Percent_Identity=46.6981132075472, Blast_Score=209, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24479; Mature: 24479

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEKRIQRYSAPERINHWIVAFCFVFAAISGLGFFFPSFNWLMNILGTPQLARILHPFV
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GVIMFAAFLLMFLRYWKHNLINREDIVWAKNIQKIVMNEEVGDTGRYNFGQKCVFWAAII
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
SLVLLLASGVVIWRPYFAPSFPIPLIRIALLVHSLAAVGLIIVIMVHIYAALWVKGTITA
HHHHHHHCCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVEGWVPAAWAKKHHPRWYREVREKQQEDKP
HHHCCCCHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKEKRIQRYSAPERINHWIVAFCFVFAAISGLGFFFPSFNWLMNILGTPQLARILHPFV
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GVIMFAAFLLMFLRYWKHNLINREDIVWAKNIQKIVMNEEVGDTGRYNFGQKCVFWAAII
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
SLVLLLASGVVIWRPYFAPSFPIPLIRIALLVHSLAAVGLIIVIMVHIYAALWVKGTITA
HHHHHHHCCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVEGWVPAAWAKKHHPRWYREVREKQQEDKP
HHHCCCCHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]