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The map label for this gene is glyS

Identifier: 157368319

GI number: 157368319

Start: 71426

End: 73495

Strand: Direct

Name: glyS

Synonym: Spro_0070

Alternate gene names: 157368319

Gene position: 71426-73495 (Clockwise)

Preceding gene: 157368318

Following gene: 157368320

Centisome position: 1.31

GC content: 59.08

Gene sequence:

>2070_bases
ATGACTCAACAGACTTTCCTGGTGGAAATCGGCACGGAAGAGCTGCCGCCGAAGGCTCTTCGTTCACTGGCAGAAGCTTT
TGCCGCGAATTTTACCGCCGAGCTCGATAGCGCCAACCTCGAACACGGCGAAGTGAGCTGGTTTGCCGCTCCGCGCCGCC
TGGCGCTGAAAGTGGCCAATTTGAGCGCTGCACAGGCCGATCGCGAAGTCGAAAAACGCGGTCCGGCCATTGCCCAGGCG
TTTGACGCCGAAGGCAAGCCAAGCAAGGCGGCCGAAGGTTGGGCACGCGGTTGCGGTATTACCGTCGATCAGGCCGAACG
CCTGGTGACCGACAAAGGTGAATGGCTGCTGTATCGCGCCCATGTTAAAGGCCAATCCGCGCAAGCGCTGCTGGCGGGCA
TGGTCAGCAATTCTCTGGCCAAGCTGCCGATCCCGAAACTAATGCGCTGGGGCGATTCCGACGTGCAGTTTGTGCGTCCA
GTGCACACCGTGACGATGCTGTTGGGTGCGGAACTGATCCCGGGCACCGTGCTGGGCATCGATTCTGCCCGTACGGTTCG
CGGCCACCGTTTCATGGGTGAAGCGGAATTCACTATCGACAATGCCGACCAATATCCGCAGATCCTGCTGGAGCGCGGTA
AGGTGATCGCCGATTACGAAACCCGTAAGGCAATGATCAAGCGCGACGCCGAGCTGGCTGCTTCGACCATTGGCGGCAAG
GCCGATCTGAGCGAAAGCCTGCTGGAAGAAGTGGCCTCGCTGGTGGAATGGCCGGTGGTGTTGACCGCCAAATTCGAAGA
GAAATTCCTGGCGGTGCCGGCGGAAGCACTGGTGTACACCATGAAAGGCGACCAGAAGTATTTCCCGGTGTATGACGCCG
CGGGCAAACTGCTGCCGAACTTTATCTTCGTGGCCAATATCGAATCCAAAGATCCGCAGCAGATTATCTCCGGTAACGAG
AAGGTAGTGCGTCCGCGTCTGGCCGATGCCGAGTTCTTCTTTAACACCGACCGCAAAAAACGTCTGGAAGACAACCTGCC
GCGTCTGGAAACCGTGCTGTTCCAACAGCAACTGGGCACCCTGCGCGACAAGACCGACCGTATTCAGGCGCTGGCGGGCT
GGGTTGCCGGTCAGATTGGTGCTGACGTTAACCACGCCACCCGTGCGGGCCTGCTGTCGAAGTGCGATCTGATGACCAAC
ATGGTCTTCGAATTCACCGACACCCAGGGCGTGATGGGCATGCACTACGCGCGTCATGACGGCGAAGCTGAAGACGTGGC
CGTTGCATTGAACGAACAGTACCAGCCGCGCTTTGCCGGTGATGCACTGCCGGAGTCGCTGGTGGCCTGTTCGCTGGCGA
TCGCCGACAAGATGGACACCTTGGCCGGTATTTTCGGCATCGGGCAACATCCGAAAGGCGATAAAGACCCGTTTGCACTA
CGCCGTGCTGCGCTGGGCGTGCTGCGTATTATCGTTGAGAAAAACCTGACGCTGGATCTGCAGACCCTGACCGAAGAGGC
CGTGCGCCTGTACGGCAGCAAGCTGACCAACGCCAAGGTGGTCGACGAGGTCGTCGAGTTTATGCTGGGCCGTTTCCGCG
CCTGGTATCAGGAAGAAGGCCACGCGGTCGACACCATTCAGGCGGTGTTGGCACGTCGTCCGACCAAACCGGCGGACTTC
GATGCGCGAGTCAAAGCGGTGACCCACTTCCGTACCCTGGAGGCGGCAGCTGCGCTGGCTGCGGCCAACAAACGCGTTTC
CAATATCCTGGCCAAATCTACCGATACGCTTAATGATCGTGTACACGCCTCGGTATTGAAAGAAGCGGCTGAGATCCAAC
TGGCGACCCATCTGGTGGTACTGCGCGATAAACTGGAGCCGTATTTTGCGGCAGGCAACTATCAGGATGCGCTGGTGGAG
CTGGCAGACCTGCGTGAGCCGGTCGATGCGTTCTTCGACAACGTGATGGTGATGGCAGAAGACGAAGCGGTACGTGTGAA
CCGTCTGACGCTGCTGAGCAAGCTGCGTGAGCTGTTCCTGCAGGTCGCGGATATCTCCGTGCTGCAATAA

Upstream 100 bases:

>100_bases
TATCCGCACGCTGACCAAAGCCGTGGCCGAGGCCTACTACGCTTCTCGCGAAGCGCTGGGCTTCCCAATGTGCAAAAAGA
ACAAGAGCTAAGAGGCAGCC

Downstream 100 bases:

>100_bases
GCGGTTCTGCTGTGAAAAGGCGCCTCAGGGCGCCTTTTTTATTGCTGTCGGCGCAGTGGGCGGTTTTGTGTCAAACCTGT
TTCTTTTAACGCTGCCAATA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MTQQTFLVEIGTEELPPKALRSLAEAFAANFTAELDSANLEHGEVSWFAAPRRLALKVANLSAAQADREVEKRGPAIAQA
FDAEGKPSKAAEGWARGCGITVDQAERLVTDKGEWLLYRAHVKGQSAQALLAGMVSNSLAKLPIPKLMRWGDSDVQFVRP
VHTVTMLLGAELIPGTVLGIDSARTVRGHRFMGEAEFTIDNADQYPQILLERGKVIADYETRKAMIKRDAELAASTIGGK
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDAAGKLLPNFIFVANIESKDPQQIISGNE
KVVRPRLADAEFFFNTDRKKRLEDNLPRLETVLFQQQLGTLRDKTDRIQALAGWVAGQIGADVNHATRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDALPESLVACSLAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGVLRIIVEKNLTLDLQTLTEEAVRLYGSKLTNAKVVDEVVEFMLGRFRAWYQEEGHAVDTIQAVLARRPTKPADF
DARVKAVTHFRTLEAAAALAAANKRVSNILAKSTDTLNDRVHASVLKEAAEIQLATHLVVLRDKLEPYFAAGNYQDALVE
LADLREPVDAFFDNVMVMAEDEAVRVNRLTLLSKLRELFLQVADISVLQ

Sequences:

>Translated_689_residues
MTQQTFLVEIGTEELPPKALRSLAEAFAANFTAELDSANLEHGEVSWFAAPRRLALKVANLSAAQADREVEKRGPAIAQA
FDAEGKPSKAAEGWARGCGITVDQAERLVTDKGEWLLYRAHVKGQSAQALLAGMVSNSLAKLPIPKLMRWGDSDVQFVRP
VHTVTMLLGAELIPGTVLGIDSARTVRGHRFMGEAEFTIDNADQYPQILLERGKVIADYETRKAMIKRDAELAASTIGGK
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDAAGKLLPNFIFVANIESKDPQQIISGNE
KVVRPRLADAEFFFNTDRKKRLEDNLPRLETVLFQQQLGTLRDKTDRIQALAGWVAGQIGADVNHATRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDALPESLVACSLAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGVLRIIVEKNLTLDLQTLTEEAVRLYGSKLTNAKVVDEVVEFMLGRFRAWYQEEGHAVDTIQAVLARRPTKPADF
DARVKAVTHFRTLEAAAALAAANKRVSNILAKSTDTLNDRVHASVLKEAAEIQLATHLVVLRDKLEPYFAAGNYQDALVE
LADLREPVDAFFDNVMVMAEDEAVRVNRLTLLSKLRELFLQVADISVLQ
>Mature_688_residues
TQQTFLVEIGTEELPPKALRSLAEAFAANFTAELDSANLEHGEVSWFAAPRRLALKVANLSAAQADREVEKRGPAIAQAF
DAEGKPSKAAEGWARGCGITVDQAERLVTDKGEWLLYRAHVKGQSAQALLAGMVSNSLAKLPIPKLMRWGDSDVQFVRPV
HTVTMLLGAELIPGTVLGIDSARTVRGHRFMGEAEFTIDNADQYPQILLERGKVIADYETRKAMIKRDAELAASTIGGKA
DLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDAAGKLLPNFIFVANIESKDPQQIISGNEK
VVRPRLADAEFFFNTDRKKRLEDNLPRLETVLFQQQLGTLRDKTDRIQALAGWVAGQIGADVNHATRAGLLSKCDLMTNM
VFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDALPESLVACSLAIADKMDTLAGIFGIGQHPKGDKDPFALR
RAALGVLRIIVEKNLTLDLQTLTEEAVRLYGSKLTNAKVVDEVVEFMLGRFRAWYQEEGHAVDTIQAVLARRPTKPADFD
ARVKAVTHFRTLEAAAALAAANKRVSNILAKSTDTLNDRVHASVLKEAAEIQLATHLVVLRDKLEPYFAAGNYQDALVEL
ADLREPVDAFFDNVMVMAEDEAVRVNRLTLLSKLRELFLQVADISVLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=86.3570391872279, Blast_Score=1212, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_SERP5 (A8G7U0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476308.1
- ProteinModelPortal:   A8G7U0
- STRING:   A8G7U0
- GeneID:   5607265
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0070
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- BioCyc:   SPRO399741:SPRO_0070-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75894; Mature: 75762

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQTFLVEIGTEELPPKALRSLAEAFAANFTAELDSANLEHGEVSWFAAPRRLALKVAN
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCEEEEECCHHHHHEECC
LSAAQADREVEKRGPAIAQAFDAEGKPSKAAEGWARGCGITVDQAERLVTDKGEWLLYRA
CHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
HVKGQSAQALLAGMVSNSLAKLPIPKLMRWGDSDVQFVRPVHTVTMLLGAELIPGTVLGI
ECCCCHHHHHHHHHHHCCHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEC
DSARTVRGHRFMGEAEFTIDNADQYPQILLERGKVIADYETRKAMIKRDAELAASTIGGK
CCCCHHCCCEEECCEEEEECCCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCC
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDAAGKLLPN
CHHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCHHHEEEEECCCCEECCHHHHHHHHCCC
FIFVANIESKDPQQIISGNEKVVRPRLADAEFFFNTDRKKRLEDNLPRLETVLFQQQLGT
EEEEEECCCCCHHHHHCCCCCEECCCCCCCHHEECCCHHHHHHCCCHHHHHHHHHHHHHH
LRDKTDRIQALAGWVAGQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHCCC
GEAEDVAVALNEQYQPRFAGDALPESLVACSLAIADKMDTLAGIFGIGQHPKGDKDPFAL
CCHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGVLRIIVEKNLTLDLQTLTEEAVRLYGSKLTNAKVVDEVVEFMLGRFRAWYQEEG
HHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
HAVDTIQAVLARRPTKPADFDARVKAVTHFRTLEAAAALAAANKRVSNILAKSTDTLNDR
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VHASVLKEAAEIQLATHLVVLRDKLEPYFAAGNYQDALVELADLREPVDAFFDNVMVMAE
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEC
DEAVRVNRLTLLSKLRELFLQVADISVLQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQQTFLVEIGTEELPPKALRSLAEAFAANFTAELDSANLEHGEVSWFAAPRRLALKVAN
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCEEEEECCHHHHHEECC
LSAAQADREVEKRGPAIAQAFDAEGKPSKAAEGWARGCGITVDQAERLVTDKGEWLLYRA
CHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
HVKGQSAQALLAGMVSNSLAKLPIPKLMRWGDSDVQFVRPVHTVTMLLGAELIPGTVLGI
ECCCCHHHHHHHHHHHCCHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEC
DSARTVRGHRFMGEAEFTIDNADQYPQILLERGKVIADYETRKAMIKRDAELAASTIGGK
CCCCHHCCCEEECCEEEEECCCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCC
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDAAGKLLPN
CHHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCHHHEEEEECCCCEECCHHHHHHHHCCC
FIFVANIESKDPQQIISGNEKVVRPRLADAEFFFNTDRKKRLEDNLPRLETVLFQQQLGT
EEEEEECCCCCHHHHHCCCCCEECCCCCCCHHEECCCHHHHHHCCCHHHHHHHHHHHHHH
LRDKTDRIQALAGWVAGQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHCCC
GEAEDVAVALNEQYQPRFAGDALPESLVACSLAIADKMDTLAGIFGIGQHPKGDKDPFAL
CCHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGVLRIIVEKNLTLDLQTLTEEAVRLYGSKLTNAKVVDEVVEFMLGRFRAWYQEEG
HHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
HAVDTIQAVLARRPTKPADFDARVKAVTHFRTLEAAAALAAANKRVSNILAKSTDTLNDR
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VHASVLKEAAEIQLATHLVVLRDKLEPYFAAGNYQDALVELADLREPVDAFFDNVMVMAE
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEC
DEAVRVNRLTLLSKLRELFLQVADISVLQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA