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The map label for this gene is gyrB [H]

Identifier: 157368284

GI number: 157368284

Start: 34375

End: 36789

Strand: Direct

Name: gyrB [H]

Synonym: Spro_0035

Alternate gene names: 157368284

Gene position: 34375-36789 (Clockwise)

Preceding gene: 157368283

Following gene: 157368285

Centisome position: 0.63

GC content: 54.78

Gene sequence:

>2415_bases
ATGTCGAATTCTTATGACTCCTCAAGTATCAAGGTATTAAAAGGGCTGGACGCGGTGCGTAAGCGCCCGGGCATGTATAT
CGGCGACACCGATGATGGCACCGGTCTGCACCACATGGTATTCGAGGTTGTGGACAACGCTATCGACGAAGCACTCGCTG
GCCACTGTAGTGACATCCAGGTGACCATCCATGCCGATAACTCCGTTTCGGTACAGGATGACGGCCGTGGTATCCCGACC
GGTATTCACGAAGAGGAAGGGGTTTCTGCGGCGCAGGTGATCATGACCGTGCTGCATGCCGGCGGCAAATTTGATGACAA
CTCCTACAAGGTATCCGGCGGTCTGCACGGCGTGGGTGTTTCTGTTGTTAACGCCTTATCCGAGAAGCTGGAACTGGTTA
TCCGTCGCGAAGGCAAGGTGCACGAACAGACCTACGCCATGGGTGAGCCACAGGCGCCGCTGAAAGTGGTCGGTGAAACC
GATCAGACCGGCACCACGGTGCGTTTCTGGCCGAGCTATGAAACCTTCACCAATAATGTTGAATTTGAGTACGATATTCT
GGCCAAGCGCCTGCGCGAACTCTCATTCCTGAACTCGGGCGTGTCTATCCGCCTGAAAGACAAACGCACCGACAAAGAAG
ACCACTTCCACTACGAAGGCGGTATCAAGGCGTTTGTTGAGTACCTGAACAAGAACAAAACCCCGATCCATCCAAACGTG
TTCTATTTCTCGACCATGAAAGATGACATCGGTGTGGAAGTGGCGCTGCAGTGGAACGACGGTTTCCAGGAAAATATCTA
CTGCTTCACCAACAACATCCCACAGCGTGATGGGGGTACCCACCTGGTCGGTTTCCGTACCGCGATGACCCGTACCCTGA
ACAGCTACATGGATAAAGAAGGCTACAGCAAAAAAGCCAAAATCAGCGCCACCGGTGACGATGCGCGTGAAGGCCTGATT
GCCGTGGTTTCGGTGAAAGTGCCGGATCCGAAGTTCTCTTCTCAGACCAAAGACAAGCTGGTTTCTTCCGAGGTGAAAAC
CGCGGTTGAAACGCTGATGAATGAAAAGCTGGTCGATTATCTGATGGAAAACCCGGGCGACGCGAAAATCGTCGTCGGCA
AAATCATCGATGCGGCGCGTGCTCGTGAAGCGGCGCGTAAAGCGCGTGAAATGACCCGTCGTAAAGGCGCATTGGATCTG
GCCGGCCTGCCGGGCAAACTGGCTGACTGTCAGGAACGCGATCCGGCGCTGTCCGAACTGTACCTGGTGGAAGGGGACTC
AGCGGGCGGCTCTGCCAAGCAGGGGCGTAACCGTAAGAACCAGGCAATCTTGCCGTTGAAAGGTAAAATCCTCAACGTTG
AGAAAGCACGCTTCGACAAGATGCTGTCTTCGCAGGAAGTGGCGACGCTGATCACTGCACTGGGCTGCGGCATTGGCCGC
GATGAGTACAACCCGGACAAACTGCGTTACCACAGCATCATTATCATGACCGATGCCGACGTCGATGGCTCGCACATCCG
TACGCTGCTGTTGACCTTCTTCTACCGTCAGATGCCGGAAATCATTGAGCGCGGCCACGTGTTCATTGCTCAGCCGCCGC
TGTACAAGGTGAAAAAAGGCAAGCAGGAACAGTACATCAAAGATGACGAAGCGATGGATCAGTACCAAATCTCTATCGCA
ATGGACGGCGCAACCCTGCACACCAACGCCAGCGCACCAGCACTTGCCGGTGAGCCATTGGAAAAACTGGTTGCCGAGCA
CTATAGCGTGCAGAAACTGATCGGTCGTATGGAGCGCCGCTACCCGCGTGCACTGCTGAACAACCTGATTTACCAGCCAA
CTTTGGCAGAGGGCGATCTGAGCGATCAGGCCAAGGTTAAAACCTGGATTGATTCGCTGGTGAAACTGCTGAACGACAAA
GAGCAGCATGGCAGCAGCTATGACTCGGTGATCTTTGAGAACCGTGAGCGCCAGATGTTTGAGCCGGTACTGCGCATCCG
TACCCACGGTGTGGATACCGACTACGCGCTGGACTTCGACTTCATCCACGGCGGCGAATATCGCAAGATCTGCGTGCTGG
GTGAGAAACTGCGCGGCCTGATCGAAGAAGATGCCTTCATCGAACGTGGTGAGCGTCGTCAACCCGTTGAAAGCTTCGAG
CAGGCATTGGAGTGGCTGGTGAAAGAGTCACGCCGTGGGCTGGCAGTTCAGCGTTATAAAGGTCTGGGTGAAATGAACCC
AGAGCAGCTGTGGGAAACCACCATGGATCCGGAAAGCCGCCGCATGCTGCGCGTGACCGTGAAGGACGCCATTGCTGCTG
ACCAACTGTTCACCACCCTGATGGGTGATGCGGTTGAGCCTCGTCGTGCCTTTATCGAAGAAAATGCCCTGAAAGCGGCA
AACATCGATATCTAA

Upstream 100 bases:

>100_bases
ACAAGTGACCGATATGGCCGGTGAAAAGGGCAAGATGTTCCGCGTGGAACAAGGTAAAATAGAGGTTCAACCACAGGACT
AAAATGAGCGAGAAACGTTG

Downstream 100 bases:

>100_bases
CGCATTGATGGCTGTAAAAATCCGCGCCGGGCGACCCGCGCGGATTTTTTTACGCCTTTTTTGGCGCTGACATTACGGGC
TGAAACGCGTTAGCATGGGG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 804; Mature: 803

Protein sequence:

>804_residues
MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCSDIQVTIHADNSVSVQDDGRGIPT
GIHEEEGVSAAQVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKLELVIRREGKVHEQTYAMGEPQAPLKVVGET
DQTGTTVRFWPSYETFTNNVEFEYDILAKRLRELSFLNSGVSIRLKDKRTDKEDHFHYEGGIKAFVEYLNKNKTPIHPNV
FYFSTMKDDIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLVGFRTAMTRTLNSYMDKEGYSKKAKISATGDDAREGLI
AVVSVKVPDPKFSSQTKDKLVSSEVKTAVETLMNEKLVDYLMENPGDAKIVVGKIIDAARAREAARKAREMTRRKGALDL
AGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVFIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIA
MDGATLHTNASAPALAGEPLEKLVAEHYSVQKLIGRMERRYPRALLNNLIYQPTLAEGDLSDQAKVKTWIDSLVKLLNDK
EQHGSSYDSVIFENRERQMFEPVLRIRTHGVDTDYALDFDFIHGGEYRKICVLGEKLRGLIEEDAFIERGERRQPVESFE
QALEWLVKESRRGLAVQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALKAA
NIDI

Sequences:

>Translated_804_residues
MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCSDIQVTIHADNSVSVQDDGRGIPT
GIHEEEGVSAAQVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKLELVIRREGKVHEQTYAMGEPQAPLKVVGET
DQTGTTVRFWPSYETFTNNVEFEYDILAKRLRELSFLNSGVSIRLKDKRTDKEDHFHYEGGIKAFVEYLNKNKTPIHPNV
FYFSTMKDDIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLVGFRTAMTRTLNSYMDKEGYSKKAKISATGDDAREGLI
AVVSVKVPDPKFSSQTKDKLVSSEVKTAVETLMNEKLVDYLMENPGDAKIVVGKIIDAARAREAARKAREMTRRKGALDL
AGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVFIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIA
MDGATLHTNASAPALAGEPLEKLVAEHYSVQKLIGRMERRYPRALLNNLIYQPTLAEGDLSDQAKVKTWIDSLVKLLNDK
EQHGSSYDSVIFENRERQMFEPVLRIRTHGVDTDYALDFDFIHGGEYRKICVLGEKLRGLIEEDAFIERGERRQPVESFE
QALEWLVKESRRGLAVQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALKAA
NIDI
>Mature_803_residues
SNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCSDIQVTIHADNSVSVQDDGRGIPTG
IHEEEGVSAAQVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKLELVIRREGKVHEQTYAMGEPQAPLKVVGETD
QTGTTVRFWPSYETFTNNVEFEYDILAKRLRELSFLNSGVSIRLKDKRTDKEDHFHYEGGIKAFVEYLNKNKTPIHPNVF
YFSTMKDDIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLVGFRTAMTRTLNSYMDKEGYSKKAKISATGDDAREGLIA
VVSVKVPDPKFSSQTKDKLVSSEVKTAVETLMNEKLVDYLMENPGDAKIVVGKIIDAARAREAARKAREMTRRKGALDLA
GLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGRD
EYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVFIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIAM
DGATLHTNASAPALAGEPLEKLVAEHYSVQKLIGRMERRYPRALLNNLIYQPTLAEGDLSDQAKVKTWIDSLVKLLNDKE
QHGSSYDSVIFENRERQMFEPVLRIRTHGVDTDYALDFDFIHGGEYRKICVLGEKLRGLIEEDAFIERGERRQPVESFEQ
ALEWLVKESRRGLAVQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALKAAN
IDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=567, Percent_Identity=26.4550264550265, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI19913408, Length=567, Percent_Identity=25.3968253968254, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI48994957, Length=804, Percent_Identity=88.3084577114428, Blast_Score=1459, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=557, Percent_Identity=40.754039497307, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535065, Length=535, Percent_Identity=27.2897196261682, Blast_Score=146, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI212645845, Length=518, Percent_Identity=24.5173745173745, Blast_Score=125, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=581, Percent_Identity=24.6127366609294, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17136538, Length=542, Percent_Identity=25.4612546125461, Blast_Score=137, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90039; Mature: 89908

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCSDIQ
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
VTIHADNSVSVQDDGRGIPTGIHEEEGVSAAQVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSEKLELVIRREGKVHEQTYAMGEPQAPLKVVGETDQTGTTVRFWPSYETFTNNV
HHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEECCCCCCCCEEEECCCHHHHCCCC
EFEYDILAKRLRELSFLNSGVSIRLKDKRTDKEDHFHYEGGIKAFVEYLNKNKTPIHPNV
CEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHCCCCCCCCCCE
FYFSTMKDDIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLVGFRTAMTRTLNSYMDKE
EEEEECCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCC
GYSKKAKISATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKTAVETLMNEKLVDY
CCCCCEEEEECCCCHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMENPGDAKIVVGKIIDAARAREAARKAREMTRRKGALDLAGLPGKLADCQERDPALSEL
HHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEE
YLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
EEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVFIAQPPLYKVKKG
CCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCC
KQEQYIKDDEAMDQYQISIAMDGATLHTNASAPALAGEPLEKLVAEHYSVQKLIGRMERR
CHHHHCCCCCCCCEEEEEEEECCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YPRALLNNLIYQPTLAEGDLSDQAKVKTWIDSLVKLLNDKEQHGSSYDSVIFENRERQMF
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHCCHHHHHH
EPVLRIRTHGVDTDYALDFDFIHGGEYRKICVLGEKLRGLIEEDAFIERGERRQPVESFE
HHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCHHHHH
QALEWLVKESRRGLAVQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTL
HHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHH
MGDAVEPRRAFIEENALKAANIDI
HCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCSDIQ
CCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
VTIHADNSVSVQDDGRGIPTGIHEEEGVSAAQVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSEKLELVIRREGKVHEQTYAMGEPQAPLKVVGETDQTGTTVRFWPSYETFTNNV
HHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEECCCCCCCCEEEECCCHHHHCCCC
EFEYDILAKRLRELSFLNSGVSIRLKDKRTDKEDHFHYEGGIKAFVEYLNKNKTPIHPNV
CEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHCCCCCCCCCCE
FYFSTMKDDIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLVGFRTAMTRTLNSYMDKE
EEEEECCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCC
GYSKKAKISATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKTAVETLMNEKLVDY
CCCCCEEEEECCCCHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMENPGDAKIVVGKIIDAARAREAARKAREMTRRKGALDLAGLPGKLADCQERDPALSEL
HHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEE
YLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
EEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVFIAQPPLYKVKKG
CCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCC
KQEQYIKDDEAMDQYQISIAMDGATLHTNASAPALAGEPLEKLVAEHYSVQKLIGRMERR
CHHHHCCCCCCCCEEEEEEEECCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YPRALLNNLIYQPTLAEGDLSDQAKVKTWIDSLVKLLNDKEQHGSSYDSVIFENRERQMF
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHCCHHHHHH
EPVLRIRTHGVDTDYALDFDFIHGGEYRKICVLGEKLRGLIEEDAFIERGERRQPVESFE
HHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCHHHHH
QALEWLVKESRRGLAVQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTL
HHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHH
MGDAVEPRRAFIEENALKAANIDI
HCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]