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The map label for this gene is recF

Identifier: 157368283

GI number: 157368283

Start: 33271

End: 34356

Strand: Direct

Name: recF

Synonym: Spro_0034

Alternate gene names: 157368283

Gene position: 33271-34356 (Clockwise)

Preceding gene: 157368282

Following gene: 157368284

Centisome position: 0.61

GC content: 58.1

Gene sequence:

>1086_bases
ATGGCTCTGACGCGTTTACTGATTAAAGATTTCCGTAATATAGAAGCTGCCGATTTGGCGCTGGCGCCGGGATTCAACTT
TCTGGTTGGCGCCAATGGCAGCGGAAAAACCAGCGTGCTTGAGGCGGTTTACACCCTTGGGCACGGGCGGGCGTTTCGCA
GCCTGCAGGCCGGGCGGGTGATCCGCCACGACCAGCCGGAGTTTATTTTGCACGGCCGTATTGACGGTGCAGAGCGTGAA
ATTTCCGTAGGATTAAGCAAGAGCCGTCAGGGGGACAGCAAGGTACGCATCGATGGCAGCGACGGCCACAAAGTGGCTGA
ACTGGCGCAGTTATTGCCGATGCAACTGATTACCCCCGAAGGTTTTACCCTGCTAAACGGTGGGCCGAAATTCCGGCGTG
CCTTCCTGGACTGGGGCTGTTTTCATAACGAACCCGGTTTTTTCACCGCCTGGAGCAACCTGAAACGGTTACTGAAGCAG
CGCAATGCCGCGCTGCGCCAGGTCAGTCGCTATGCGCAGATCCGGGCCTGGGATCAGGAACTGATCCCACTGGCCGAGCG
TATCAGCGAGTGGCGGGCAGCTTACAGCGACGCGATTGCCGCGGATATTACCGCGACATGCGCGCAGTTTTTGCCCGAAT
TTGCCCTGAGTTTCTCTTTCCAGCGTGGCTGGGACAAAGAGAGCGACTACGGAGAGCTGCTGGAGCGCCAGTTTGAGCGT
GACAGGGCGTTAACCTATACCGCGGTGGGGCCGCATAAGGCGGACTTCCGCATTCGTGCCGACGGCACGCCGGTAGAGGA
TTTGTTATCGCGCGGTCAGCTTAAACTGCTGATGTGCGCGTTGCGCTTAGCGCAGGGTGAGTTTCTCACCCGGCAGAGCG
GGCGGCGTTGCCTGTATCTGATTGATGATTTTGCCTCCGAGCTGGACACCGGCCGTCGCCGGTTGCTGGCCGATCGCCTG
AAAGCCACCCAGGCCCAGGTCTTTGTCAGTGCGGTAAGCGCTGAACAAGTGACCGATATGGCCGGTGAAAAGGGCAAGAT
GTTCCGCGTGGAACAAGGTAAAATAGAGGTTCAACCACAGGACTAA

Upstream 100 bases:

>100_bases
GTAAGCGCCTGGGGATAAGCTAGCTGGCCGCCTAGCTGCAACTTGAAATCCATAGGGTATATAAGGTTGAATAACTTACC
GTCATGGGTAAAGAGATTGC

Downstream 100 bases:

>100_bases
AATGAGCGAGAAACGTTGATGTCGAATTCTTATGACTCCTCAAGTATCAAGGTATTAAAAGGGCTGGACGCGGTGCGTAA
GCGCCCGGGCATGTATATCG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MALTRLLIKDFRNIEAADLALAPGFNFLVGANGSGKTSVLEAVYTLGHGRAFRSLQAGRVIRHDQPEFILHGRIDGAERE
ISVGLSKSRQGDSKVRIDGSDGHKVAELAQLLPMQLITPEGFTLLNGGPKFRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
RNAALRQVSRYAQIRAWDQELIPLAERISEWRAAYSDAIAADITATCAQFLPEFALSFSFQRGWDKESDYGELLERQFER
DRALTYTAVGPHKADFRIRADGTPVEDLLSRGQLKLLMCALRLAQGEFLTRQSGRRCLYLIDDFASELDTGRRRLLADRL
KATQAQVFVSAVSAEQVTDMAGEKGKMFRVEQGKIEVQPQD

Sequences:

>Translated_361_residues
MALTRLLIKDFRNIEAADLALAPGFNFLVGANGSGKTSVLEAVYTLGHGRAFRSLQAGRVIRHDQPEFILHGRIDGAERE
ISVGLSKSRQGDSKVRIDGSDGHKVAELAQLLPMQLITPEGFTLLNGGPKFRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
RNAALRQVSRYAQIRAWDQELIPLAERISEWRAAYSDAIAADITATCAQFLPEFALSFSFQRGWDKESDYGELLERQFER
DRALTYTAVGPHKADFRIRADGTPVEDLLSRGQLKLLMCALRLAQGEFLTRQSGRRCLYLIDDFASELDTGRRRLLADRL
KATQAQVFVSAVSAEQVTDMAGEKGKMFRVEQGKIEVQPQD
>Mature_360_residues
ALTRLLIKDFRNIEAADLALAPGFNFLVGANGSGKTSVLEAVYTLGHGRAFRSLQAGRVIRHDQPEFILHGRIDGAEREI
SVGLSKSRQGDSKVRIDGSDGHKVAELAQLLPMQLITPEGFTLLNGGPKFRRAFLDWGCFHNEPGFFTAWSNLKRLLKQR
NAALRQVSRYAQIRAWDQELIPLAERISEWRAAYSDAIAADITATCAQFLPEFALSFSFQRGWDKESDYGELLERQFERD
RALTYTAVGPHKADFRIRADGTPVEDLLSRGQLKLLMCALRLAQGEFLTRQSGRRCLYLIDDFASELDTGRRRLLADRLK
ATQAQVFVSAVSAEQVTDMAGEKGKMFRVEQGKIEVQPQD

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=355, Percent_Identity=81.6901408450704, Blast_Score=615, Evalue=1e-177,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_SERP5 (A8G7Q4)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476272.1
- ProteinModelPortal:   A8G7Q4
- SMR:   A8G7Q4
- STRING:   A8G7Q4
- GeneID:   5603525
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0034
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   SPRO399741:SPRO_0034-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 40493; Mature: 40361

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTRLLIKDFRNIEAADLALAPGFNFLVGANGSGKTSVLEAVYTLGHGRAFRSLQAGRV
CHHHHHHHHHHCCCCHHHEEECCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHCCC
IRHDQPEFILHGRIDGAEREISVGLSKSRQGDSKVRIDGSDGHKVAELAQLLPMQLITPE
CCCCCCCEEEEECCCCCCHHEEECCCCCCCCCCEEEEECCCCHHHHHHHHHCCHHEECCC
GFTLLNGGPKFRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVSRYAQIRAWDQE
CCEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIPLAERISEWRAAYSDAIAADITATCAQFLPEFALSFSFQRGWDKESDYGELLERQFER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
DRALTYTAVGPHKADFRIRADGTPVEDLLSRGQLKLLMCALRLAQGEFLTRQSGRRCLYL
CCCEEEEECCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCEEEEE
IDDFASELDTGRRRLLADRLKATQAQVFVSAVSAEQVTDMAGEKGKMFRVEQGKIEVQPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCC
D
C
>Mature Secondary Structure 
ALTRLLIKDFRNIEAADLALAPGFNFLVGANGSGKTSVLEAVYTLGHGRAFRSLQAGRV
HHHHHHHHHHCCCCHHHEEECCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHCCC
IRHDQPEFILHGRIDGAEREISVGLSKSRQGDSKVRIDGSDGHKVAELAQLLPMQLITPE
CCCCCCCEEEEECCCCCCHHEEECCCCCCCCCCEEEEECCCCHHHHHHHHHCCHHEECCC
GFTLLNGGPKFRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVSRYAQIRAWDQE
CCEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIPLAERISEWRAAYSDAIAADITATCAQFLPEFALSFSFQRGWDKESDYGELLERQFER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
DRALTYTAVGPHKADFRIRADGTPVEDLLSRGQLKLLMCALRLAQGEFLTRQSGRRCLYL
CCCEEEEECCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCEEEEE
IDDFASELDTGRRRLLADRLKATQAQVFVSAVSAEQVTDMAGEKGKMFRVEQGKIEVQPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA