Click here to switch to the map view.

The map label for this gene is intE [H]

Identifier: 157159388

GI number: 157159388

Start: 1262112

End: 1263230

Strand: Reverse

Name: intE [H]

Synonym: EcE24377A_1248

Alternate gene names: 157159388

Gene position: 1263230-1262112 (Counterclockwise)

Preceding gene: 157155362

Following gene: 157158875

Centisome position: 25.37

GC content: 45.93

Gene sequence:

>1119_bases
ATGTCACCGAGGCCACGAAAAAACAGCACTGACGTAACCGGTCTTTACGAAAAGTTTGATCGCAGAACTGGCAGGGTTTA
CTACCAGTATAAAAACCCTGTGACTGGAAAATTTCACGGTCTCGGAACAGACAAAGGCACAGCAGAAAAAATCGCTTCCA
CAGCCAATCAGCGAATAGCTGCAGCAGAAGCTGAATATTTCATGCGCAAAATTGATGAAAGTCCGTCAGCAACAAAACGT
CGGGGTATCAGATTAAAGGCATGGGTTGATCGATATCTGAAAATACAGGACGCGCGACTGAAAAATGGAGATATTGCAGC
TACAACTCACAAAGAAAAAGCCCGAATGGCTGCACACCTGGTTTCCCGTCTGGGAAACCATCCATTGAAAGAACTGGAAG
TAAGAGACTTTGCATTAATACTGGATGAGTGGCTGGATAAAGACATGGTCAGCACAGCGAGAGTAAATCGTGGATTATGG
GTTGATATTTATAAAGAAGCACAGCATGCAGGGGAAGTTCCTCCTGGATGGAATCCTCCGGAGGCCACCCGTAAACCGAT
CCCTAAAGTAACCAGAGCCAGACTCACCCTGGAAGACTGGCAAAAAATTTACAACGCAACGCCTGAAGAACACTTTATCC
GTAACGCAATGCTCCTTGCGATTGTTACTGGTCAGCGCCGTGATGACATTTGCCACATGCGTTTTTCAGATGTGTGGAAC
GAACACTTGCATATCACCCAGGGAAAAACCGGAATGCGTCTGGCGTTACCGCTTACACTACGCTGTGATGCTATTGGGAT
AACGTTAAAAGAAGTTATTGATGGGTGCCGAGACAAAATATTAAGTCCATATCTAATCCATAGTCGGCACCAGAAACAAC
CAAAGCCGATGAGTAAAGACAACCTGAGCGACTACTTCGCCAAAGCACGGGATCTGGCTAGGATAATTCCACCAGCAGGA
AAAACTCCGCCAACATTTCATGAACAACGCTCCCTGTCAGAACGACTGTACCGTGCGCAGGGTATTGATACAAAAACATT
ACTGGGACATAAAGTCCAGGCAACCACCGATCGTTATAACGATACTCGTGGTCAGGAATGGGTTAAATTGGTTGTTTGA

Upstream 100 bases:

>100_bases
CTGATAGATCGCCGGGCAGTATTTGTTGGACCTGGTGAAACGGGAATTGCGCCGGAAATTCATACTGGCGATGATGATGC
ACTGAAGGAGATTTTAACTC

Downstream 100 bases:

>100_bases
CGAAATAAATACAGCTGAAAAATGTTGATATTGCTTTGCTAGTGTAAAAACCCCGGCATCAACCGGGGTTCGGAGACTAA
TCTTGATCAGGTTCCTGCTT

Product: phage integrase family protein

Products: NA

Alternate protein names: Int(Lambda); Prophage e14 integrase [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSPRPRKNSTDVTGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGTAEKIASTANQRIAAAEAEYFMRKIDESPSATKR
RGIRLKAWVDRYLKIQDARLKNGDIAATTHKEKARMAAHLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTLEDWQKIYNATPEEHFIRNAMLLAIVTGQRRDDICHMRFSDVWN
EHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDKILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLARIIPPAG
KTPPTFHEQRSLSERLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVV

Sequences:

>Translated_372_residues
MSPRPRKNSTDVTGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGTAEKIASTANQRIAAAEAEYFMRKIDESPSATKR
RGIRLKAWVDRYLKIQDARLKNGDIAATTHKEKARMAAHLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTLEDWQKIYNATPEEHFIRNAMLLAIVTGQRRDDICHMRFSDVWN
EHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDKILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLARIIPPAG
KTPPTFHEQRSLSERLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVV
>Mature_371_residues
SPRPRKNSTDVTGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGTAEKIASTANQRIAAAEAEYFMRKIDESPSATKRR
GIRLKAWVDRYLKIQDARLKNGDIAATTHKEKARMAAHLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLWV
DIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTLEDWQKIYNATPEEHFIRNAMLLAIVTGQRRDDICHMRFSDVWNE
HLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDKILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLARIIPPAGK
TPPTFHEQRSLSERLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVV

Specific function: Integrase from the cryptic lambdoic prophage e14. Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the h

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1787386, Length=377, Percent_Identity=48.0106100795756, Blast_Score=344, Evalue=6e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016177
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR015094
- InterPro:   IPR023109
- InterPro:   IPR013087 [H]

Pfam domain/function: PF09003 Phage_integ_N; PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 42664; Mature: 42533

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRPRKNSTDVTGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGTAEKIASTANQRIA
CCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHH
AAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDARLKNGDIAATTHKEKARMAAHL
HHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHH
VSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLWVDIYKEAQHAGEVPPGWNPP
HHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCC
EATRKPIPKVTRARLTLEDWQKIYNATPEEHFIRNAMLLAIVTGQRRDDICHMRFSDVWN
HHHCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHC
EHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDKILSPYLIHSRHQKQPKPMSKD
CCEEEECCCCCCEEEEEEEEEECHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCC
NLSDYFAKARDLARIIPPAGKTPPTFHEQRSLSERLYRAQGIDTKTLLGHKVQATTDRYN
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHCC
DTRGQEWVKLVV
CCCCHHHHEECC
>Mature Secondary Structure 
SPRPRKNSTDVTGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGTAEKIASTANQRIA
CCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHH
AAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDARLKNGDIAATTHKEKARMAAHL
HHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHH
VSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLWVDIYKEAQHAGEVPPGWNPP
HHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCC
EATRKPIPKVTRARLTLEDWQKIYNATPEEHFIRNAMLLAIVTGQRRDDICHMRFSDVWN
HHHCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHC
EHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDKILSPYLIHSRHQKQPKPMSKD
CCEEEECCCCCCEEEEEEEEEECHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCC
NLSDYFAKARDLARIIPPAGKTPPTFHEQRSLSERLYRAQGIDTKTLLGHKVQATTDRYN
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHCC
DTRGQEWVKLVV
CCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]