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The map label for this gene is trpS [H]

Identifier: 157159383

GI number: 157159383

Start: 3827321

End: 3828325

Strand: Reverse

Name: trpS [H]

Synonym: EcE24377A_3853

Alternate gene names: 157159383

Gene position: 3828325-3827321 (Counterclockwise)

Preceding gene: 157158678

Following gene: 157154799

Centisome position: 76.88

GC content: 52.04

Gene sequence:

>1005_bases
ATGACTAAGCCCATCGTTTTTAGTGGCGCACAGCCCTCAGGTGAATTGACCATTGGTAACTACATGGGTGCGCTGCGTCA
GTGGGTAAACATGCAGGATGACTACCATTGCATTTACTGTATCGTTGACCAACACGCGATCACCGTGCGCCAGGATGCAC
AGAAGCTGCGTAAAGCGACGCTGGATACGCTGGCCTTGTATCTGGCTTGTGGTATCGATCCTGAGAAAAGCACCATTTTT
GTTCAGTCCCACGTACCAGAACATGCGCAGTTAGGCTGGGCACTGAACTGCTATACCTACTTCGGCGAACTGAGCCGCAT
GACCCAGTTTAAAGATAAATCTGCGCGTTATGCCGAGAACATCAACGCTGGTCTGTTTGACTATCCGGTGCTGATGGCTG
CGGACATCCTGCTGTATCAAACTAATCTGGTACCGGTGGGTGAAGACCAGAAACAGCACCTGGAACTGAGTCGCGATATC
GCCCAGCGTTTCAACGCGCTGTATGGCGAGATCTTTAAGGTGCCGGAGCCGTTTATTCCGAAATCTGGCGCGCGCGTAAT
GTCGCTGCTGGAGCCGACCAAGAAGATGTCCAAGTCTGACGATAACCGCAATAACGTTATCGGCCTGCTGGAAGATCCGA
AATCGGTAGTGAAGAAAATCAAACGCGCGGTCACTGACTCCGACGAGCCGCCGGTAGTTCGCTACGATGTGCAGAACAAA
GCGGGCGTTTCCAACCTGCTGGATATCCTTTCTGCGGTAACGGGCCAGAGCATCCTAGAACTGGAAAAACAGTTCGAAGG
CAAGATGTATGGTCATCTGAAAGGTGAAGTGGCTGATGCCGTTTCCGGTATGCTGACTGAATTGCAGGAACGCTATCACC
GTTTCCGCAACGATGAAGCCTTCCTGCAACAGGTGATGAAAGACGGCGCAGAAAAAGCCAGTGTACACGCTTCCCGTACG
CTGAAAGCGGTGTACGAAGCGATTGGTTTTGTGGCGAAGCCGTAA

Upstream 100 bases:

>100_bases
GAGGCTATCGATCTCAGCCAGCCTGATGTAATTTATCAGTCTATAAATGACCTTCTGCCCGCATTAGGGCTTCCGCATAG
CGAAAATCAGGAATCGAAAA

Downstream 100 bases:

>100_bases
TATCAAAACGCTCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAGCAGGCAAGCGCCAGTGGGAAT
TACCCTTTGCTCGCATTGTC

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQHAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIF
VQSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKIKRAVTDSDEPPVVRYDVQNK
AGVSNLLDILSAVTGQSILELEKQFEGKMYGHLKGEVADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRT
LKAVYEAIGFVAKP

Sequences:

>Translated_334_residues
MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQHAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIF
VQSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKIKRAVTDSDEPPVVRYDVQNK
AGVSNLLDILSAVTGQSILELEKQFEGKMYGHLKGEVADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRT
LKAVYEAIGFVAKP
>Mature_333_residues
TKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQHAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFV
QSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDIA
QRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKIKRAVTDSDEPPVVRYDVQNKA
GVSNLLDILSAVTGQSILELEKQFEGKMYGHLKGEVADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTL
KAVYEAIGFVAKP

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=331, Percent_Identity=43.202416918429, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI41352700, Length=179, Percent_Identity=52.5139664804469, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI1789786, Length=334, Percent_Identity=99.4011976047904, Blast_Score=694, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71982800, Length=334, Percent_Identity=36.2275449101796, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI71982793, Length=334, Percent_Identity=36.2275449101796, Blast_Score=207, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6320474, Length=345, Percent_Identity=38.5507246376812, Blast_Score=226, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24666151, Length=334, Percent_Identity=43.7125748502994, Blast_Score=248, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 37482; Mature: 37351

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQHAITVRQDAQKLRKAT
CCCCEEEECCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
LDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYAEN
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
INAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDIAQRFNALYGEIFKVPEPFIP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKIKRAVTDSDEPPVVRYDVQNK
CCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCC
AGVSNLLDILSAVTGQSILELEKQFEGKMYGHLKGEVADAVSGMLTELQERYHRFRNDEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQHAITVRQDAQKLRKAT
CCCEEEECCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
LDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYAEN
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
INAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDIAQRFNALYGEIFKVPEPFIP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKIKRAVTDSDEPPVVRYDVQNK
CCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCC
AGVSNLLDILSAVTGQSILELEKQFEGKMYGHLKGEVADAVSGMLTELQERYHRFRNDEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]