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The map label for this gene is yfhK [H]

Identifier: 157159317

GI number: 157159317

Start: 2839456

End: 2840838

Strand: Reverse

Name: yfhK [H]

Synonym: EcE24377A_2841

Alternate gene names: 157159317

Gene position: 2840838-2839456 (Counterclockwise)

Preceding gene: 157155948

Following gene: 157155220

Centisome position: 57.05

GC content: 52.57

Gene sequence:

>1383_bases
ATGCTGGCATTTTTGCTAATTCTACTACCCCTGTTGGTGCTGGCATGGCAAGCCTGGCAAAGCCTGAATGCGCTTAGCGA
TCAGGCGGCGCTGGTTAACCGCACTACGCTTATTGATGCCCGGCGTAGTGAAGCGATGACCAACGCGGCGCTGGAGATGG
AGCGTAGCTACCGTCAGTATTGCGTGCTGGACGATCCTACGCTTGCGAAGGTTTATCAAAGCCAGCGCAAGCGTTACAGC
GAAATGCTCGATGCCCACGCAGGCGTACTGCCAGACGATAAACTTTACCAGGCATTGCGTCAGGATTTGAACAATCTGGC
CCAACTTCAGTGTAACAACAGTGGTCCCGATGCTGCCGCCGCCGCGCGTCTTGAAGCCTTTGCCAGTGCCAATACCGAAA
TGGTGCAGGCCACGCGCACAGTGGTGTTCTCTCGTGGGCAGCAACTTCAGCGTGAAATCGCCGAACGTGGGCAATATTTT
GGTTGGCAATCGCTGGTGCTATTTCTGGTGAGTCTGGTGATGGTGCTACTTTTCACGCGGATGATTATCGGGCCGGTGAA
AAATATCGAGCGCATGATCAACCGTCTGGGGGAAGGGCGTTCTCTGGGCAATAGCGTTTCGTTCAGTGGACCGAGCGAGT
TACGCTCGGTTGGGCAACGTATTCTTTGGTTAAGTGAGCGCCTGTCATGGCTGGAATCCCAACGCCATCAATTTTTAAGA
CATTTATCTCATGAATTAAAAACGCCACTGGCGAGTATGCGCGAGGGCACTGAATTACTGGCTGACCAGGTTGTCGGGCC
GCTTACGCCAGAGCAAAAAGAGGTGGTGAGCATTCTTGATAGCAGCAGCCGCAATTTGCAAAAACTGATCGAACAACTGC
TTGATTACAACCGTAAACAGGCGGACAGTGCGGTGGAACTGGAGAATGTTGAATTAGCACCGCTGGTGGAGACAGTGGTT
TCTGCTCATAGCCTGCCCGCACGGGCTAAAATGATGCATACCGACGTCGATCTCAAAGCAACAGCTTGCCTGGCGGAGCC
AATGCTGCTGATGAGCGTACTGGATAATCTTTACTCCAATGCGGTGCACTACGGGGCTGAATCCGGTAACATTTGCCTTC
GCAGCAGCTTACATGGTGCACGGGTTTATATTGATGTCATCAATACAGGCACACCCATTCCGCAAGAGGAACGCGCCATG
ATCTTCGAACCCTTTTTTCAAGGAAGCCACCAGCGAAAAGGGGCGGTGAAGGGCAGCGGTCTGGGATTAAGCATTGCCAG
GGATTGTATTCGCCGTATGCAAGGGGAACTGTATCTGGTCGACGAGAGCGGGCAAGACGTTTGTTTCCGCATTGAATTGC
CGTCGTCGAAAAACACGAAATAA

Upstream 100 bases:

>100_bases
TCTGAACTTCCCCTCGGTTAGCATCAGGCTATTCGCGTCTGACGAGAGTAACACCTTGAAACGCTGGCCTGTTTTTCCCC
GCTCATTACGACAACTGGTA

Downstream 100 bases:

>100_bases
TGCTAACAGCCTATCCCATCAGGCTATTTTATTTGCCAGTTCGGCCCCGGGCAGTGCTCGAAATCCTCACGTACGACGTG
TACGCTCCGGTTTCTCCGCG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYS
EMLDAHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSRGQQLQREIAERGQYF
GWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGNSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLR
HLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETVV
SAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSLHGARVYIDVINTGTPIPQEERAM
IFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESGQDVCFRIELPSSKNTK

Sequences:

>Translated_460_residues
MLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYS
EMLDAHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSRGQQLQREIAERGQYF
GWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGNSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLR
HLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETVV
SAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSLHGARVYIDVINTGTPIPQEERAM
IFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESGQDVCFRIELPSSKNTK
>Mature_460_residues
MLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYS
EMLDAHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSRGQQLQREIAERGQYF
GWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGNSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLR
HLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETVV
SAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSLHGARVYIDVINTGTPIPQEERAM
IFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESGQDVCFRIELPSSKNTK

Specific function: Probable member of a two-component regulatory system yfhA/yfhK. May activate yfhA by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082128, Length=460, Percent_Identity=99.7826086956522, Blast_Score=941, Evalue=0.0,
Organism=Escherichia coli, GI1790861, Length=302, Percent_Identity=31.7880794701987, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI145693157, Length=228, Percent_Identity=29.8245614035088, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081816, Length=408, Percent_Identity=25.9803921568627, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=29.4871794871795, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789808, Length=291, Percent_Identity=24.3986254295533, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1789149, Length=354, Percent_Identity=24.2937853107345, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=231, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790346, Length=297, Percent_Identity=23.9057239057239, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=24.1992882562278, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=24.6575342465753, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI48994928, Length=226, Percent_Identity=26.9911504424779, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790300, Length=232, Percent_Identity=31.4655172413793, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788549, Length=260, Percent_Identity=26.1538461538462, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786783, Length=296, Percent_Identity=24.3243243243243, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=25.974025974026, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787894, Length=249, Percent_Identity=26.1044176706827, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788279, Length=331, Percent_Identity=23.8670694864048, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51413; Mature: 51413

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSEAMTNAALEMERSYRQY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCE
CVLDDPTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAA
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCHHH
AARLEAFASANTEMVQATRTVVFSRGQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTR
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MIIGPVKNIERMINRLGEGRSLGNSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQ
HHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHH
ADSAVELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSN
CCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVHYGAESGNICLRSSLHGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSG
HHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
LGLSIARDCIRRMQGELYLVDESGQDVCFRIELPSSKNTK
CCHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSEAMTNAALEMERSYRQY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCE
CVLDDPTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAA
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCHHH
AARLEAFASANTEMVQATRTVVFSRGQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTR
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MIIGPVKNIERMINRLGEGRSLGNSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQ
HHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHH
ADSAVELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSN
CCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVHYGAESGNICLRSSLHGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSG
HHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
LGLSIARDCIRRMQGELYLVDESGQDVCFRIELPSSKNTK
CCHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]