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The map label for this gene is phnM [H]

Identifier: 157159054

GI number: 157159054

Start: 4647968

End: 4649104

Strand: Reverse

Name: phnM [H]

Synonym: EcE24377A_4649

Alternate gene names: 157159054

Gene position: 4649104-4647968 (Counterclockwise)

Preceding gene: 157157230

Following gene: 157157976

Centisome position: 93.36

GC content: 60.69

Gene sequence:

>1137_bases
ATGATTATCAATAACGTCAAACTGGTGCTGGAAAACGAAGTGGTACACGGTTCGCTGGAGGTGCAGGATGGCGAAATCCG
CGCCTTTGCCGAAAGCCAGAGCCGCCTGCCAGAAGCGATGGACGGCGAAGGTGGCTGGCTACTACCCGGCCTGATTGAGC
TGCATACCGACAACCTCGATAAATTCTTCACCCCGCGCCCGAAAGTCGACTGGCCCGCCCATTCGGCGATGAGCAGCCAC
GACGCGCTGATGGTGGCAAGCGGCATCACCACCGTACTGGACGCCGTGGCGATTGGTGATGTGCGCGACGGCGGCGATCG
GCTGGAGAATCTGGAGAAGATGATCAACGCCATCGAAGAGACGCAAAAACGCGGCGTCAACCGCGCCGAGCACCGCCTGC
ACCTGCGCTGCGAACTGCCGCATCACACCACACTGCCGCTGTTTGAAAAACTGGTTCAGCGCGAACCGGTGACGCTGGTG
TCGCTGATGGACCACTCACCGGGCCAGCGCCAGTTCGCTAACCGCGAGAAGTATCGCGAATATTATCAGGGCAAATACTC
CCTCACCGATGCGCAGATGCAGCAGTACGAAGAAGAGCAATTGGCGCTCGCCGCACGCTGGTCGCAGCCGAATCGCGAAT
CCATCGCCGCCCTGTGCCGCGCGCGACAAATTGCGCTCGCCAGCCACGATGACGCCACGCACGCCCACGTTGCCGAATCC
CACCAACTTGGCAGCGTGATCGCCGAATTTTCCACCACGTTCGAAGCGGCGGAAGCCTCGCGTAAGCATGGCATGAACGT
GCTGATGGGTGCGCCAAATATCGTGCGTGGCGGCTCGCACTCCGGCAATGTGGCGGCCAGTGAACTGGCGCAGCTTGGTC
TGCTGGATATCCTCTCTTCCGACTACTACCCCGCCAGCCTGCTGGATGCGGCGTTCCGCGTCGCCGATGACGAGAGCAAC
CGCTTTACGCTACAGCAGGCGGTGAGGCTGGTGACCAAAAATCCGGCGCAGGCGCTGAATCTTCAGGATCGCGGGGTGAT
TGGCGAGGGTAAACGCGCTGACCTGGTGCTGGCGCATCGCCAGGGCAATCACATTCATATCGACCACGTCTGGCGTCAGG
GTAAAAGGGTGTTCTGA

Upstream 100 bases:

>100_bases
GCGAAGCCAAAACCCGTGGCGCGGCCATCGTCGGCATCTTCCATGACGAAGCCGTACGTAATGACGTCGCCGACCGCTTG
CACCCAATGGGAGCCTCTGC

Downstream 100 bases:

>100_bases
TGATGGGAAAACTGATTTGGTTAATGGGGCCGTCCGGCTCCGGGAAAGACAGCCTGCTGGCGGAACTCCGCCTGCGGGAA
CAAACGCAGTTACTGGTGGC

Product: phosphonate metabolism protein PhnM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSH
DALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAALCRARQIALASHDDATHAHVAES
HQLGSVIAEFSTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESN
RFTLQQAVRLVTKNPAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF

Sequences:

>Translated_378_residues
MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSH
DALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAALCRARQIALASHDDATHAHVAES
HQLGSVIAEFSTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESN
RFTLQQAVRLVTKNPAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF
>Mature_378_residues
MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSH
DALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAALCRARQIALASHDDATHAHVAES
HQLGSVIAEFSTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESN
RFTLQQAVRLVTKNPAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3454

COG function: function code P; Metal-dependent hydrolase involved in phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790533, Length=378, Percent_Identity=97.6190476190476, Blast_Score=762, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059
- InterPro:   IPR012696 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: NA

Molecular weight: Translated: 42054; Mature: 42054

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLD
CEECCEEEEEECCEEECEEEECCCCHHEEHHHHHCCCCCCCCCCCEECCCHHEEECCCHH
KFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEE
HHCCCCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
TQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLVSLMDHSPGQRQFANREKYRE
HHHHCCCHHHCEEEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
YYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAALCRARQIALASHDDATHAHVAES
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHH
HQLGSVIAEFSTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSS
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
DYYPASLLDAAFRVADDESNRFTLQQAVRLVTKNPAQALNLQDRGVIGEGKRADLVLAHR
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCEEEEEC
QGNHIHIDHVWRQGKRVF
CCCEEEHHHHHCCCCCCC
>Mature Secondary Structure
MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLD
CEECCEEEEEECCEEECEEEECCCCHHEEHHHHHCCCCCCCCCCCEECCCHHEEECCCHH
KFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEE
HHCCCCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
TQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLVSLMDHSPGQRQFANREKYRE
HHHHCCCHHHCEEEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
YYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAALCRARQIALASHDDATHAHVAES
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHH
HQLGSVIAEFSTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSS
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
DYYPASLLDAAFRVADDESNRFTLQQAVRLVTKNPAQALNLQDRGVIGEGKRADLVLAHR
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCEEEEEC
QGNHIHIDHVWRQGKRVF
CCCEEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]