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The map label for this gene is hofQ

Identifier: 157158971

GI number: 157158971

Start: 3834143

End: 3835381

Strand: Reverse

Name: hofQ

Synonym: EcE24377A_3861

Alternate gene names: 157158971

Gene position: 3835381-3834143 (Counterclockwise)

Preceding gene: 157157124

Following gene: 157158436

Centisome position: 77.02

GC content: 54.4

Gene sequence:

>1239_bases
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGTCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Upstream 100 bases:

>100_bases
GGCTGGACAATTTTACAGCTGACGCCAGACGTACTAACGCTGGGTACCGGGACAAACTGCGAACCGCCACAATGGTTGTG
GCAACGGCAAGGAGATACAA

Downstream 100 bases:

>100_bases
ACAGCCGTAAAAGCGGTAATGTTTTTATGCTGAACGTGTTTCATCTATTTGACGCGCGCAGGTATTTAGCATACAAGGAG
TACCGATTTGAGAGTTGGTG

Product: outer membrane porin HofQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Sequences:

>Translated_412_residues
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE
>Mature_412_residues
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Specific function: Required for the use of extracellular DNA as a nutrient. Could be the porin responsible for transport of DNA across the outer membrane

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family

Homologues:

Organism=Escherichia coli, GI1789793, Length=412, Percent_Identity=100, Blast_Score=823, Evalue=0.0,
Organism=Escherichia coli, GI87082242, Length=332, Percent_Identity=28.3132530120482, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOFQ_ECOLI (P34749)

Other databases:

- EMBL:   Z19601
- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B65134
- RefSeq:   AP_004399.1
- RefSeq:   NP_417850.1
- ProteinModelPortal:   P34749
- SMR:   P34749
- STRING:   P34749
- EnsemblBacteria:   EBESCT00000002651
- EnsemblBacteria:   EBESCT00000015768
- GeneID:   947901
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3354
- KEGG:   eco:b3391
- EchoBASE:   EB2036
- EcoGene:   EG12113
- eggNOG:   COG4796
- GeneTree:   EBGT00050000010859
- HOGENOM:   HBG482665
- OMA:   SISLQYA
- ProtClustDB:   PRK10560
- BioCyc:   EcoCyc:EG12113-MONOMER
- Genevestigator:   P34749
- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845
- InterPro:   IPR001814
- PRINTS:   PR00811
- PRINTS:   PR01032
- SMART:   SM00965
- TIGRFAMs:   TIGR02515

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN

EC number: NA

Molecular weight: Translated: 44717; Mature: 44717

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSL
CHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCEEEECCCCCCEEEE
HLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENR
EEECCCHHHHHHHHHHHCCCEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCC
SITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDNKTALSALEQWVAQMDLPVGQ
EEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHCCCCCE
VELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
EEEEEEEEEECHHHHHHCCCEEEECCHHHCCCCCEEEECCCCCEEEEEEEECCEEEEECC
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEA
CEEEEEEHHHHHHHHHCCEECCCHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHH
VLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLAL
HHCCCCCHHHHHCCCEEEEEEECCCCCHHHHHHCCCCEEEEEHHHCCEEEEECCCCEEEE
GGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERRELVVFITPRLVSSE
CCEEEECCCCCCCCCCCEECCCHHHHHHHCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSL
CHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCEEEECCCCCCEEEE
HLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENR
EEECCCHHHHHHHHHHHCCCEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCC
SITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDNKTALSALEQWVAQMDLPVGQ
EEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHCCCCCE
VELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
EEEEEEEEEECHHHHHHCCCEEEECCHHHCCCCCEEEECCCCCEEEEEEEECCEEEEECC
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEA
CEEEEEEHHHHHHHHHCCEECCCHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHH
VLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLAL
HHCCCCCHHHHHCCCEEEEEEECCCCCHHHHHHCCCCEEEEEHHHCCEEEEECCCCEEEE
GGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERRELVVFITPRLVSSE
CCEEEECCCCCCCCCCCEECCCHHHHHHHCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1309529; 9278503; 7901733