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The map label for this gene is trmJ

Identifier: 157158953

GI number: 157158953

Start: 2812505

End: 2813242

Strand: Reverse

Name: trmJ

Synonym: EcE24377A_2817

Alternate gene names: 157158953

Gene position: 2813242-2812505 (Counterclockwise)

Preceding gene: 157156907

Following gene: 157158615

Centisome position: 56.5

GC content: 55.28

Gene sequence:

>738_bases
ATGCTGCAAAATATTCGAATTGTGCTGGTGGAGACGTCACACACCGGCAATATGGGTTCTGTTGCCCGTGCCATGAAAAC
AATGGGATTAACCAATCTGTGGCTGGTTAATCCACTGGTGAAACCCGACTCCCAGGCGATTGCCCTGGCAGCAGGGGCCA
GCGATGTGATTGGTAATGCTCACATCGTCGATACGCTCGACGAAGCGTTAGCCGGTTGTAGCCTGGTGGTGGGCACCAGC
GCACGCTCCCGCACGCTGCCGTGGCCGATGCTCGACCCGCGCGAATGCGGTCTGAAAAGCGTCGCTGAAGCGGCAAATAC
CCCTGTGGCGCTGGTGTTTGGTCGTGAGCGCGTCGGCTTGACCAATGAAGAGTTGCAGAAATGCCATTATCATGTCGCGA
TTGCGGCTAACCCGGAATACAGCTCGCTGAACCTGGCGATGGCGGTTCAGGTTATTGCCTATGAAGTGCGCATGGCCTGG
CTGGCAACTCAGGAAAACGGCGAGCAGGTCGAGCATGAAGAGACGCCGTATCCGCTGGTCGATGATCTGGAGCGTTTTTA
TGGTCATCTGGAACAAACGCTGCTGGCAACCGGTTTTATCCGTGAAAACCATCCGGGGCAGGTGATGAATAAATTGCGCC
GTCTGTTTACCCGTGCGCGCCCGGAAAGCCAGGAATTGAATATCCTGCGCGGGATTCTGGCTTCTATTGAGCAGCAGAAT
AAAGGTAATAAGGCCTGA

Upstream 100 bases:

>100_bases
GTTAAAGAACGGGAAAACGGCGCAGAGTATAGCAGCGAGATCGGCATCTGTCTTCGTTTTATGATAAGATGCGTGAATAA
TCTTTAGATGAAGAAAGACA

Downstream 100 bases:

>100_bases
GGGCTAAGCCAGATAAATGCACGGACGGTCCCCTCGCCCCTTCGGGGAGAGGGTTAGGGTGAGGGGAAGAGGCCAGCGCT
AATGCGAATAATTGCCCTCA

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
KGNKA

Sequences:

>Translated_245_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
KGNKA
>Mature_245_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
KGNKA

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family

Homologues:

Organism=Escherichia coli, GI1788881, Length=245, Percent_Identity=100, Blast_Score=505, Evalue=1e-145,
Organism=Escherichia coli, GI1790865, Length=153, Percent_Identity=37.2549019607843, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMJ_ECOK1 (A1AE69)

Other databases:

- EMBL:   CP000468
- RefSeq:   YP_853673.1
- ProteinModelPortal:   A1AE69
- SMR:   A1AE69
- STRING:   A1AE69
- EnsemblBacteria:   EBESCT00000039480
- GeneID:   4492356
- GenomeReviews:   CP000468_GR
- KEGG:   ecv:APECO1_3993
- eggNOG:   COG0565
- GeneTree:   EBGT00050000010186
- HOGENOM:   HBG724367
- OMA:   FGREHAG
- ProtClustDB:   PRK15114
- BioCyc:   ECOL405955:APECO1_3993-MONOMER
- GO:   GO:0005737
- InterPro:   IPR004384
- InterPro:   IPR001537
- PIRSF:   PIRSF004808
- TIGRFAMs:   TIGR00050

Pfam domain/function: PF00588 SpoU_methylase

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26919; Mature: 26919

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: BINDING 114-114 BINDING 134-134 BINDING 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNA
CCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEEECCCHHHCCCC
HIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEEEEECCCCCCC
TNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWLATQENGEQVEHEETPYPLV
CHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHH
DDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
KGNKA
CCCCC
>Mature Secondary Structure
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNA
CCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEEECCCHHHCCCC
HIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEEEEECCCCCCC
TNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWLATQENGEQVEHEETPYPLV
CHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHH
DDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
KGNKA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA