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The map label for this gene is ydeH

Identifier: 157158898

GI number: 157158898

Start: 1743671

End: 1744561

Strand: Reverse

Name: ydeH

Synonym: EcE24377A_1744

Alternate gene names: 157158898

Gene position: 1744561-1743671 (Counterclockwise)

Preceding gene: 157154870

Following gene: 157156486

Centisome position: 35.03

GC content: 44.78

Gene sequence:

>891_bases
ATGATCAAGAAAACAACGGAAATTGATGCCATCTTGTTAAATCTCAATAAGGCTATCGATGCCCACTACCAGTGGCTGGT
GAGTATGTTTCACAGCGTGGTCGCGAGAGATGCCAGTAAGCCAGAAATAACGGATAACCATTCTTATGGACTGTGCCAGT
TTGGTCGGTGGATTGATCATCTGGGGCCACTCGATAACGATGAATTACCTTACGTTCGGCTAATGGATTCTGCCCATCAA
CATATGCATAACTGTGGTCGGGAATTAATGCTGGCTATTGTTGAAAATCACTGGCAGGACGCGCATTTCGACGCCTTTCA
GGAGGGGTTGCTTTCTTTTACTGCGGCATTAACCGATTACAAAATTTATTTGCTGACGATCCGTAGCAATATGGATGTTT
TGACGGGATTGCCGGGGCGTCGGGTTCTTGATGAATCCTTTGATCATCAGTTACGCAACGCTGAGCCTCTGAATCTTTAT
TTAATGTTGTTGGATATTGACCGATTTAAATTGGTTAATGATACCTACGGGCATTTAATCGGCGATGTAGTATTACGCAC
CCTGGCAACTTACTTAGCCAGTTGGACGCGTGATTACGAAACGGTTTATCGCTACGGGGGCGAAGAATTTATCATTATTG
TCAAAGCGGCTAATGATGAAGAAGCATGTCGTGCAGGTGTCAGAATTTGCCAGTTAGTCGATAACCATGCCATCACGCAT
TCTGAAGGGCATATCAACATTACCGTGACAGCAGGTGTCAGTCGCGCATTTCCTGAAGAGCCTCTGGATGTGGTCATTGG
AAGAGCAGACCGGGCAATGTATGAGGGTAAGCAAACCGGAAGAAATCGCTGCATGTTTATTGATGAACAAAATGTGATTA
ACCGAGTTTAA

Upstream 100 bases:

>100_bases
AGATGACTCATAACTTCGGATTTCTATGAAAATATTGACGTAATTTTGCTTTTCTCTCGTTAGAATAGCGCGCACAAGGA
ACTGTGAAAAAGGAGTGGCA

Downstream 100 bases:

>100_bases
GCTCCGTTTAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATCCGTTATCAGTGCAGGAAAATGCCTGTTAGCG
TAAAAGCAAAACACAAATCT

Product: hypothetical protein

Products: NA

Alternate protein names: DGC

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQ
HMHNCGRELMLAIVENHWQDAHFDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
SEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV

Sequences:

>Translated_296_residues
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQ
HMHNCGRELMLAIVENHWQDAHFDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
SEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV
>Mature_296_residues
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQ
HMHNCGRELMLAIVENHWQDAHFDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
SEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV

Specific function: A diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returnes to normal when residues 206-207 are both mutated to Ala. Overexpression also leads to a reduction in flagellar abundance and a 20-fold increase in c-di- GMP

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain

Homologues:

Organism=Escherichia coli, GI1787816, Length=296, Percent_Identity=100, Blast_Score=616, Evalue=1e-178,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=34.1614906832298, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=32.7485380116959, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787056, Length=191, Percent_Identity=32.4607329842932, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=35.625, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=30.5555555555556, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=31.7073170731707, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=29.6969696969697, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=33.1428571428571, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082007, Length=130, Percent_Identity=36.1538461538462, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081977, Length=162, Percent_Identity=31.4814814814815, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YDEH_ECOLI (P31129)

Other databases:

- EMBL:   M96235
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B64908
- RefSeq:   AP_002156.1
- RefSeq:   NP_416052.1
- ProteinModelPortal:   P31129
- SMR:   P31129
- IntAct:   P31129
- STRING:   P31129
- EnsemblBacteria:   EBESCT00000004046
- EnsemblBacteria:   EBESCT00000018411
- GeneID:   946075
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1528
- KEGG:   eco:b1535
- EchoBASE:   EB1596
- EcoGene:   EG11643
- eggNOG:   COG2199
- GeneTree:   EBGT00050000008776
- HOGENOM:   HBG416179
- OMA:   EEFIICM
- ProtClustDB:   PRK09894
- BioCyc:   EcoCyc:EG11643-MONOMER
- BioCyc:   MetaCyc:EG11643-MONOMER
- Genevestigator:   P31129
- InterPro:   IPR001054
- InterPro:   IPR000160
- SMART:   SM00267
- TIGRFAMs:   TIGR00254

Pfam domain/function: PF00990 GGDEF; SSF55073 A/G_cyclase

EC number: =2.7.7.65

Molecular weight: Translated: 33864; Mature: 33864

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50887 GGDEF

Important sites: ACT_SITE 208-208 BINDING 173-173 BINDING 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDH
CCCCCHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAHFDAFQEGLLSFTAALTDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
KIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLYLMLLDIDRFKLVNDTYGHLI
EEEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHCHHHHHHH
GDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEE
SEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV
CCCEEEEEEEECCCCCCCCCCHHHEECCHHHHHHCCHHCCCCCEEEEECHHHHCCC
>Mature Secondary Structure
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDH
CCCCCHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAHFDAFQEGLLSFTAALTDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
KIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLYLMLLDIDRFKLVNDTYGHLI
EEEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHCHHHHHHH
GDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEE
SEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV
CCCEEEEEEEECCCCCCCCCCHHHEECCHHHHHHCCHHCCCCCEEEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383113; 9097039; 9278503