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The map label for this gene is ligB [H]

Identifier: 157158891

GI number: 157158891

Start: 4135427

End: 4137115

Strand: Reverse

Name: ligB [H]

Synonym: EcE24377A_4148

Alternate gene names: 157158891

Gene position: 4137115-4135427 (Counterclockwise)

Preceding gene: 157155212

Following gene: 157157093

Centisome position: 83.08

GC content: 52.81

Gene sequence:

>1689_bases
GTGATGATGAAAGTATGGATGGCGATATTAATAAGTATCTTGTGCTGGCAATCATCTGTGTGGGCGGTCTGTCCGGCCTG
GTCGCCAGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTGGGACGATGACTACTGGAAGGAAG
GAAAAAGTGAGGTGGAAGACGGTGTTTACGATCAGTTAAGCGCCCGTCTTACGCAGTGGCAACGCTGCTTTGGGAGCGAG
CCCCGCGACGTGATGATGCCGCCTTTAAATGGTGCGGTTATGCATCCGGTTGCCCATACGGGTGTACGCAAAATGGTGGA
TAAAAACGCATTAAGTCTGTGGATGCGAGAACGTAGCGATCTTTGGGTGCAGCCAAAAGTTGATGGCGTTGCGGTAACCC
TGGTTTATCGGGACGGGAAACTGAACAAAGCAATCAGTCGCGGTAATGGCCTGAAAGGCGAGGACTGGACGCAGAAAGTT
AGCTTAATTTCCGCTGTGCCGCAAACCGTTAGCGGGCCTTTAGCCAACAGTACGCTTCAGGGGGAAATATTTCTCCAGCG
CGAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAAGTTGCTGGCCTGATGATGCGCCAGGACGATA
GCGACACGCTGAATTCTCTGGGCGTTTTTGTCTGGGCATGGCCGGATGGACCGCAGTTAATGACCGATCGTTTAAAGGAG
CTGGCTACCGCAGGTTTTACTCTAACGCAGAGGTATACCCGTGCGGTTAAAAATGCTGATGAGGTTGCGCGCGTACGCAA
TGAGTGGTGGAAAGCGAAATTACCCTTCGTCACCGATGGCGTGGTTGTGCGAGGGGCGAAAGAACCCGAATCCCGCCATT
GGCTACCAGGCCAGGCAGAATGGCTGGTGGCCTGGAAATATCAACCTGTAGCTCAGGTTGCCGAAGTGAAGGCGATTCAG
TTTGCGGTGGGTAAGAGCGGTAAAATATCGGTGGTTGCGTCACTCGCACCTGTCATGCTGGATGATAAAAAAGTCCAGCG
GGTGAATATTGGTTCCGTCAGGCGCTGGCAGGAGTGGGATATTGCGCCTGGTGATCAGATCCTCGTCAGCCTTGCCGGTC
AGGGTATTCCTCGCATTGATGATGTGGTCTGGCGCGGTGCAGAACGTACAAAACCGACACCACCAGAAAACCGCTTTAAC
TCATTGACCTGCTACTTTGCTTCTGATGTTTGTCAGGAACAGTTCATTTCACGCTTAGTCTGGCTGGGGTCAAAACAGGT
TCTGGGGCTGGATGGTATTGGTGAGGCCGGCTGGCGCGCGCTGCATCAGACTCATCGCTTTGAGCATATCTTTTCCTGGC
TTTTATTAACGCCAGAGCAATTACAGAACACGCCGGGGATCGCGAAAAGTAAAAGTGCGCAGCTATGGCATCGGTTTAAT
CTGGCTCGTAAGCAGCCTTTTACTCGCTGGGTGATGGCAATGGGAATACCGCTAACCCGGGCGGCGCTTAATGCCAGTGA
TGAACGGTCCTGGTCGCAACTTTTATTTAGCACGGAGCAGTTCTGGCAGCAGCTGCCGGGGACTGGATCGGGGCGCGCCA
GACAGGTTATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGGCTGCCCAGCAGATCACAGGTTTT
GAACCTTAG

Upstream 100 bases:

>100_bases
AATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTG
CCTGACTACCTACAGCGGAG

Downstream 100 bases:

>100_bases
TGTCCTTCGTGGCTGTAGTAAAAGACCGGTAATCCCAGCTTAAGGCGTAGCGCCAGTAAGCGGGCAAAAAAGCCGAATAC
CAGGGTGGAGATAATTACCA

Product: NAD-dependent DNA ligase LigB

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] B [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MMMKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSE
PRDVMMPPLNGAVMHPVAHTGVRKMVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKV
SLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDRLKE
LATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQ
FAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFN
SLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHRFN
LARKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGF
EP

Sequences:

>Translated_562_residues
MMMKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSE
PRDVMMPPLNGAVMHPVAHTGVRKMVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKV
SLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDRLKE
LATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQ
FAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFN
SLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHRFN
LARKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGF
EP
>Mature_562_residues
MMMKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSE
PRDVMMPPLNGAVMHPVAHTGVRKMVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKV
SLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDRLKE
LATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQ
FAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFN
SLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHRFN
LARKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGF
EP

Specific function: Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction [H]

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082305, Length=560, Percent_Identity=98.75, Blast_Score=1130, Evalue=0.0,
Organism=Escherichia coli, GI1788750, Length=518, Percent_Identity=23.7451737451737, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018239
- InterPro:   IPR020923
- InterPro:   IPR004150
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 63571; Mature: 63571

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVED
CHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHH
GVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRKMVDKNALSLWMRERSD
HHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCC
LWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISAVPQTVSGPLANSTLQ
EEECCCCCCEEEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
GEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDRLKE
CEEEEECCCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCHHHHHHHHHH
LATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAE
HHHCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCEEEECCCCCCCCCCCCCCCC
WLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWD
EEEEEECCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCHHEEECCCCCCCCCCCC
IAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCYFASDVCQEQFISRLV
CCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHH
WLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHRFN
HCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHH
LARKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWK
HHHCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ENAQIKKLGSWLAAQQITGFEP
CCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMMKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVED
CHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHH
GVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRKMVDKNALSLWMRERSD
HHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCC
LWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISAVPQTVSGPLANSTLQ
EEECCCCCCEEEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
GEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDRLKE
CEEEEECCCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCHHHHHHHHHH
LATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAE
HHHCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCEEEECCCCCCCCCCCCCCCC
WLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWD
EEEEEECCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCHHEEECCCCCCCCCCCC
IAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCYFASDVCQEQFISRLV
CCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHH
WLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHRFN
HCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHH
LARKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWK
HHHCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ENAQIKKLGSWLAAQQITGFEP
CCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA