| Definition | Escherichia coli E24377A, complete genome. |
|---|---|
| Accession | NC_009801 |
| Length | 4,979,619 |
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The map label for this gene is cas3 [H]
Identifier: 157158852
GI number: 157158852
Start: 3063187
End: 3065844
Strand: Reverse
Name: cas3 [H]
Synonym: EcE24377A_3063
Alternate gene names: 157158852
Gene position: 3065844-3063187 (Counterclockwise)
Preceding gene: 157155640
Following gene: 157156933
Centisome position: 61.57
GC content: 50.68
Gene sequence:
>2658_bases GTGACTTTTTTTGATTTCTGGGGAAAAACCCGACGCGGCGAGAAAGACGGGGGCGACGACTATCACCTTCTTTGCTGGCA TTCGCTGGATGTAGCCGCGATGGGCTATTTAATGGTTAAAAGAAATTGCTTCGGGCTGGCTGATTATTTTCGTCAATTAG GGATTTCTGACAAGGAACAGGCGGCTCAATTTTTCGCCTGGTTGCTGTGCTGGCACGATATTGGAAAATTTGCCCGCTCT TTTCAACAACTTTACCTGCCCCCTGAACTCAAGATTCAGGAAAGTGCCAGAAAAAATTACGAAAAAATCTCTCATTCAAC GCTGGGCTACTGGTTGTGGAATCATTATTTAAGTGAATGTCAGGAGTTACTTCCTTCATCTTCGCTCTCTCCGCGCAAAC TTAGGCGAGTGATAGAAATGTGGATGCCCGTGACGACAGGGCATCATGGTCGGCCCCCTGATCGTATGGATGAATTGGAT AATTTTCTGCCTGAAGACAAAGCTGCAGCACGAGATTTTCTCCTTGAAATCAAACCACTGTTTCCGCTCATAGAGATCCC CGCATTCTGGGATGATGATGAGGGCATTGAACTTATAAAACACCTTTCCTGGTATATCTCTGCAACCGTCGTGCTCGCAG ACTGGACGGGTTCGTCAACGCGATTTTTTCCACGCGTCGCACACCCAATGGATATTAAAGGTTACTGGCAGAAAACTTTA ATTCAGGCTCAAAACGCCTTAACCGTCTTTCCTCTAAAAGCAAAAGTCGCGCCTTTCAACGGAATTAATACGCTGTTTCC TTTTATTGAGAATCCGACACCGTTACAGCAAAAGGTGCTGGATCTGGATATCAGCCAGCAAGGACCGCAGTTATTTATTC TGGAAGATGTGACTGGCGCAGGTAAAACAGAAGCGGCGCTTATCCTGGCGCACAGGTTGATAGCTGCGGGGAAAGCTCAG GGGCTGTTTTTTGGTCTGCCAACAATGGCAACGGCCAATGCCATGTACGATCGGCTGGTCAAAACCTGGCTTGCTTTCTA TTCGCCAGAATCCCGCCCCAGCCTGGTGCTGGCACATAGTGCCCGCACATTAATGGACCGCTTTAATGAATCACTCTGGT CTGGTGATTTAGTCGGGTCAGAAGAACCGGATGAACAAACGTTCAGTCAGGGATGTGCGGCCTGGTTTGCCGACAGTAAC AAGAAGGCGTTGCTGGCTGAAATTGGCGTCGGCACGCTGGATCAGGCGATGATGGCGGTGATGCCGTTCAAACATAATAA TCTGCGGCTTCTGGGGCTGAGTAACAAAATCCTGCTGGCTGATGAGATCCACGCCTGTGACGCTTACATGTCGTGCATTC TTGAAGGGTTGATCGAGCGGCAGGCGCGTGGCGGAAACAGCGTTATTTTGCTTTCTGCCACGTTATCTCAACAGCAGCGC GACAAACTCGTCGCTGCCTTTGCGCGGGGTACAGAGGGCCTGCAAGAAGCTCCGTTCCTGGAAAAGGATGATTACCCCTG GCTGACGCATGTCACGAAATCCGATGTGAACTCACACCGGGTAGCGACGCGCAAAGACGTTGAGCGTAGCGTCAGCGTGG GTTGGCTTCATAGTGAACAAGAGTGTATTGCGCGTATCGAATCGGCGGTAAGTCAGGGGAAATGCATCGCCTGGATCCGG AATTCTGTCGATGACGCTATTAAGGTTCATCGTCAGCTGCTTGCCCGCGGCGTCATTCCTGCTTCCAGCCTTTCACTTTT TCATAGCCGCTTTGCTTTTAGCGATCGCCAGCGAATTGAAACGGAGACACTGGCACGCTTTGGTAAATATTGTAGTTTAC AGAGAGCGTCGCAAGTCATTGTTTGTACACAAGTAATTGAGCAAAGCGTGGATATTGACCTGGACGAAATGATCTCCGAC CTGGCCCCTGTTGATTTGCTGATTCAGCGAGCAGGACGATTACAGCGGCATATCCGCGATATTAATGGTCAGTTAAAGCG TGACGGAAAGGACGAGCGTTCCCCCCCTGAATTGCTGATTCTGGCTCCCGTCTGGGACGACTCTCCTGGCGACGAATGGT TTGGCAGTGCCATGCGTAACAGTGCATTTGTCTATCCCGATCATGGGCGAATCTGGCTGACGCAGCGTGTGCTGCGTGAG CAAGGTGCTATTCAGATGCCACACGCAGCCCGGCTTCTTATTGAATCCGTCTACGGTGAGGACGTGGTAATGCCGGAAGG ATTTGCCCGCAGCGAGCAGGAGCAAGTGGGCAAATATTACTGCGATCGCGCAATGGCTAAAAAGTTTGTGCTGAACTTCA GGCCCGGCTATGCCGCCAATATCAACGATTACCTCCCGGAAAAACTGTCGACACGTCTGGCTGAGGAGTCTGTTTCCCTG TGGCTGGCTACCTGTATTGACGGTGTGGTGAAGCCTTATGCCACCGGTGCTCACGCATGGGAAATGAGCGTTGTCAGAGT GCGCCGAAGCTGGTGGAAAAAACATCGGGATGAGTTTTCTTTACTGGAGGGAGAGGCGTTCAGGCTGTGGTGCATTGAAC AGCGACAAGATCCGGAGATGGCAAACGTGATTTTAGTCAACGATGACGAAAGTTGCGGGTATTCGGCTACGGAAGGATTG ATTGGCAAGGTTGGTTGA
Upstream 100 bases:
>100_bases TTTATTCATAATGTAAATGTTTTATTGGCTGATATATATCACAGATAATTTTTAAAATATGCGTAAATATCCTTTAAGTT TACTGAAGGATAAAAATATT
Downstream 100 bases:
>100_bases GATGTCTGCGACAGGACTCTCTGAATAGTTGATACACCGAAAAGGATGGAAAGATGGACAACCGACCTGGGATGGTCTTT TTTACGAGAGGATGCGTATG
Product: CRISPR-associated helicase Cas3
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 885; Mature: 884
Protein sequence:
>885_residues MTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARS FQQLYLPPELKIQESARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELD NFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTL IQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSN KKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQR DKLVAAFARGTEGLQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIR NSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQVIEQSVDIDLDEMISD LAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLRE QGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSL WLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGL IGKVG
Sequences:
>Translated_885_residues MTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARS FQQLYLPPELKIQESARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELD NFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTL IQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSN KKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQR DKLVAAFARGTEGLQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIR NSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQVIEQSVDIDLDEMISD LAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLRE QGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSL WLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGL IGKVG >Mature_884_residues TFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSF QQLYLPPELKIQESARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELDN FLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLI QAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQG LFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSNK KALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRD KLVAAFARGTEGLQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRN SVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQVIEQSVDIDLDEMISDL APVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQ GAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSLW LATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLI GKVG
Specific function: Unknown
COG id: COG1203
COG function: function code R; Predicted helicases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 helicase C-terminal domain [H]
Homologues:
Organism=Escherichia coli, GI1789119, Length=812, Percent_Identity=28.8177339901478, Blast_Score=262, Evalue=6e-71,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006483 - InterPro: IPR014001 - InterPro: IPR011545 - InterPro: IPR001650 - InterPro: IPR006474 - InterPro: IPR014021 [H]
Pfam domain/function: PF00270 DEAD [H]
EC number: NA
Molecular weight: Translated: 100302; Mature: 100170
Theoretical pI: Translated: 6.20; Mature: 6.20
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.7 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 4.1 %Cys+Met (Translated Protein) 1.7 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 4.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQ CCCHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH AAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQESARKNYEKISHSTLGYWLWNHYLSEC HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH QELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPL HHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC FPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTL CCCEECCCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH IQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGA HHHCCCEEEEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCC GKTEAALILAHRLIAAGKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHS CCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHH ARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAV HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH MPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQR CCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHCHHHH DKLVAAFARGTEGLQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQ HHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCCHH ECIARIESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIE HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHH TETLARFGKYCSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRD HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHH INGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLRE HCCHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHH QGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAAN CCCCCCCHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC INDYLPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFS CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHH LLEGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG HCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCHHCCCCCCCC >Mature Secondary Structure TFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQ CCHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH AAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQESARKNYEKISHSTLGYWLWNHYLSEC HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH QELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPL HHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC FPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTL CCCEECCCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH IQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGA HHHCCCEEEEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCC GKTEAALILAHRLIAAGKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHS CCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHH ARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAV HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH MPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQR CCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHCHHHH DKLVAAFARGTEGLQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQ HHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCCHH ECIARIESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIE HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHH TETLARFGKYCSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRD HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHH INGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLRE HCCHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHH QGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAAN CCCCCCCHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC INDYLPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFS CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHH LLEGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG HCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCHHCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9278503; 2005873; 7984428 [H]