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The map label for this gene is ybiY

Identifier: 157158814

GI number: 157158814

Start: 911924

End: 912823

Strand: Reverse

Name: ybiY

Synonym: EcE24377A_0895

Alternate gene names: 157158814

Gene position: 912823-911924 (Counterclockwise)

Preceding gene: 157155396

Following gene: 157155776

Centisome position: 18.33

GC content: 54.11

Gene sequence:

>900_bases
ATGATTTTCAATATTCAGCGCTACTCGACCCATGATGGCCCCGGTATCCGCACGGTCGTATTTCTTAAAGGCTGTTCGCT
GGGCTGCCGCTGGTGTCAGAACCCGGAAAGCCGCGCCCGCACGCAGGATCTGCTGTATGACGCACGACTGTGTCTGGAAG
GCTGCGAGCTGTGCGCTAAGGCCGCGCCGGAAGTGATTGAGCGCGCGCTGAATGGTTTGCTTATTCATCGGGAAAAGTTA
ACCCCGGAGCATCTGACGGCGTTAACCGACTGCTGTCCGACACAGGCATTAACCGTGTGTGGTGAAGTGAAAAGCGTTGA
GGAGATCATGACGACCGTTCTGCGCGATAAACCGTTTTATGATCGCAGCGGCGGCGGTTTAACGCTTTCGGGTGGTGAAC
CCTTTATGCAGCCGGAAATGGCGATGGCGCTACTGCAAGCCAGCCACGAGGCCGGCATTCATACTGCGGTAGAAACCTGT
CTGCATGTGCCGTGGAAATATATCGCCCCTTCTCTGCCCTATATCGATCTGTTTCTTGCCGATTTAAAACACGTTGCCGA
CGCGCCGTTTAAACAGTGGACCGACGGTAACGCCGCCAGGGTGCTGGATAACCTGAAAAAACTCGCCGCAGCGGGCAAAA
AAATCATTATCCGCGTGCCGCTGATTCAGGGCTTTAATGCCGACGAAACCTCTGTAAAAGCCATTACCGATTTTGCCGCC
GACGAGCTGCACGTTGGCGAAATTCATTTTCTGCCCTACCACACGCTGGGCATCAACAAATATCACTTACTTAATCTGCC
CTATGACGCCCCGGAAAAACCGCTTGATGCGCCAGAACTGCTCGACTTTGCCCAGCAGTATGCCTGCCAGAAAGGGTTAA
CCGCGACCTTACGAGGATAA

Upstream 100 bases:

>100_bases
ATGTAGACTATCACGGGAAAACTCTCTGAACAGTTGACGCACATCAATCTAACTTTCATTCGAAAGTATTTTAATGTTTG
AACGAAACAGAGAGGCAACT

Downstream 100 bases:

>100_bases
CAACCATGACCACACTGAAACTGGACACGCTCAGCGACCGCATTAAAGCGCACAAAAATGCGCTGGTGCATATTGTGAAA
CCGCCAGTCTGTACCGAGCG

Product: glycyl-radical activating family protein

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 3; PFL-activating enzyme 3

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKAAPEVIERALNGLLIHREKL
TPEHLTALTDCCPTQALTVCGEVKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETSVKAITDFAA
DELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG

Sequences:

>Translated_299_residues
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKAAPEVIERALNGLLIHREKL
TPEHLTALTDCCPTQALTVCGEVKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETSVKAITDFAA
DELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG
>Mature_299_residues
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKAAPEVIERALNGLLIHREKL
TPEHLTALTDCCPTQALTVCGEVKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETSVKAITDFAA
DELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family

Homologues:

Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=100, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1790389, Length=301, Percent_Identity=35.8803986710963, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787130, Length=171, Percent_Identity=32.7485380116959, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790839, Length=231, Percent_Identity=25.5411255411255, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PFLE_ECOLI (P75794)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H64819
- RefSeq:   AP_001455.1
- RefSeq:   NP_415345.2
- ProteinModelPortal:   P75794
- SMR:   P75794
- DIP:   DIP-11444N
- MINT:   MINT-1313356
- STRING:   P75794
- EnsemblBacteria:   EBESCT00000000218
- EnsemblBacteria:   EBESCT00000017433
- GeneID:   945445
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0808
- KEGG:   eco:b0824
- EchoBASE:   EB3243
- EcoGene:   EG13470
- eggNOG:   COG1180
- GeneTree:   EBGT00050000009897
- HOGENOM:   HBG554871
- ProtClustDB:   CLSK881195
- BioCyc:   EcoCyc:G6427-MONOMER
- Genevestigator:   P75794
- GO:   GO:0005737
- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197
- PIRSF:   PIRSF000371
- TIGRFAMs:   TIGR02494

Pfam domain/function: PF04055 Radical_SAM

EC number: =1.97.1.4

Molecular weight: Translated: 33038; Mature: 33038

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAK
CEEECEECCCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMTTVLRDKPFY
HHHHHHHHHHHHHEEEHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETCLHVPWKYIAPSLPYIDLFLA
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCCCHHHHHHH
DLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETSVKAITDFAA
HHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHC
DELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG
CCEEECEEEEECEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCC
>Mature Secondary Structure
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAK
CEEECEECCCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMTTVLRDKPFY
HHHHHHHHHHHHHEEEHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETCLHVPWKYIAPSLPYIDLFLA
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCCCHHHHHHH
DLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETSVKAITDFAA
HHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHC
DELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG
CCEEECEEEEECEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503