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The map label for this gene is fadA [H]

Identifier: 157158775

GI number: 157158775

Start: 4352994

End: 4354157

Strand: Reverse

Name: fadA [H]

Synonym: EcE24377A_4364

Alternate gene names: 157158775

Gene position: 4354157-4352994 (Counterclockwise)

Preceding gene: 157158390

Following gene: 157157494

Centisome position: 87.44

GC content: 57.65

Gene sequence:

>1164_bases
ATGGAACAGGTTGTCATTGTCGATGCAATTCGCACCCCGATGAGCCGTTCGAAGGGCGGTGCTTTTCGTAACGTGCGTGC
AGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTT
ACTGGGGTTGTGTGCAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCGCTGCTGGCAGAAGTACCACAC
TCTGTCCCGGCGGTTACCGTCAATCGCTTGTGTGGTTCATCCATGCAGGCACTGCATGATGCAGCACGAATGATCATGAG
CGGCGATGCGCAGGCATGTCTGGTTGGCGGCGTGGAGCATATGGGCCATGTGCCGATGAGTCACGGCGTCGATTTTCACC
CCGGCCTGAGCCGCAATGTCGCCAAAGCGGCGGGCATGATGGGCTTAACGGCAGAAATGCTGGCGCGTATGCACGGTATC
AGCCGTGAAATGCAGGATGCCTTTGCCGCGCGGTCACACGCCCGCGCCTGGGCCGCCACGCAGTCGGGTGCATTTAAAAA
TGAAATTATCCCGACCGGTGGTCACGATGCCGACGGCGTCCTGAAGCAGTTTAATTACGACGAAGTGATTCGCCCGGAAA
CCACCGTGGAAGCCCTCGCCACGCTGCGTCCGGCGTTTGATCCAGTAAGCGGTACGGTAACGGCGGGTACATCTTCTGCG
CTTTCCGATGGCGCAGCTGCCATGCTGGTGATGAGTGAAAGCCGCGCCCATGAATTAGGTCTTAAGCCGCGCGCCCGTGT
GCGTTCGATGGCGGTCGTTGGTTGTGACCCAGCGATTATGGGTTACGGCCCGGTTCCGGCCTCAAAACTGGCGCTGAAAA
AAGCGGGGCTTTCTGTCAGTGATATCGGCGTGTTTGAAATGAATGAAGCCTTTGCCGCGCAGATCCTGCCATGTATTAAA
GATCTGGGACTGATTGAGCAGATTGACGAGAAGATCAACCTCAACGGTGGTGCGATCGCGCTGGGTCATCCGCTGGGCTG
TTCCGGTGCGCGTATCAGCACCACGCTGCTGAATCTGATGGAACGCAAAGACGTTCAGTTTGGTCTGGCGACGATGTGTA
TCGGTCTGGGTCAGGGTATTGCGACGGTGTTTGAGCGGGTTTAA

Upstream 100 bases:

>100_bases
GGAAGGTCTGCGTAATAAAGCGCGTCATAACGAACCGTACTATCCTCCGGTTGAGCCAGCCCGTCCGGTTGGCGACCTGA
AAACGGCTTAAGGAGTCACA

Downstream 100 bases:

>100_bases
CCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCTGCACCAATGTAGGCCTGATAAGACGCGCCAGCG
TCGCATCAGGCATCGTGCAC

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEQVVIVDAIRTPMSRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMSGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPAIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIK
DLGLIEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV

Sequences:

>Translated_387_residues
MEQVVIVDAIRTPMSRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMSGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPAIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIK
DLGLIEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV
>Mature_387_residues
MEQVVIVDAIRTPMSRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMSGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPAIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIK
DLGLIEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=43.4343434343434, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=38.2278481012658, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI148539872, Length=400, Percent_Identity=38.5, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=34.2657342657343, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=34.3283582089552, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI194353979, Length=388, Percent_Identity=32.2164948453608, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI48994986, Length=387, Percent_Identity=98.1912144702842, Blast_Score=777, Evalue=0.0,
Organism=Escherichia coli, GI1787663, Length=405, Percent_Identity=45.1851851851852, Blast_Score=317, Evalue=8e-88,
Organism=Escherichia coli, GI1788554, Length=405, Percent_Identity=43.4567901234568, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=41.1471321695761, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1788683, Length=418, Percent_Identity=35.6459330143541, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=41.3965087281795, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=33.7209302325581, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17535921, Length=403, Percent_Identity=33.7468982630273, Blast_Score=190, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI25147385, Length=399, Percent_Identity=31.5789473684211, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=392, Percent_Identity=27.5510204081633, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6322031, Length=391, Percent_Identity=39.8976982097187, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6325229, Length=408, Percent_Identity=34.8039215686275, Blast_Score=189, Evalue=9e-49,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=40.547263681592, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI17648125, Length=402, Percent_Identity=39.3034825870647, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=34.032634032634, Blast_Score=202, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24640423, Length=410, Percent_Identity=31.219512195122, Blast_Score=161, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40834; Mature: 40834

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQVVIVDAIRTPMSRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQ
CCCEEEEHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMSGDAQACLVGGVEH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEHHCCHHH
MGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAAT
HCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHH
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPAIMGYGPVPASKLALKKAGLSVS
HCCCCEEEEEEECCHHHHCCCCCHHHHHHEEEECCCCHHCCCCCCCHHHHHHHHCCCCCH
DIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLM
HCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
ERKDVQFGLATMCIGLGQGIATVFERV
HHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MEQVVIVDAIRTPMSRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQ
CCCEEEEHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMSGDAQACLVGGVEH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEHHCCHHH
MGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAAT
HCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHH
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPAIMGYGPVPASKLALKKAGLSVS
HCCCCEEEEEEECCHHHHCCCCCHHHHHHEEEECCCCHHCCCCCCCHHHHHHHHCCCCCH
DIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLM
HCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
ERKDVQFGLATMCIGLGQGIATVFERV
HHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA