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The map label for this gene is rutB

Identifier: 157158711

GI number: 157158711

Start: 1149821

End: 1150516

Strand: Reverse

Name: rutB

Synonym: EcE24377A_1129

Alternate gene names: 157158711

Gene position: 1150516-1149821 (Counterclockwise)

Preceding gene: 157155454

Following gene: 157155569

Centisome position: 23.1

GC content: 54.89

Gene sequence:

>696_bases
ATGATGACGACCTTAACCGCTCGACCGGAAGCCATTACCTTCGATCCGCAGCAAAGTGCGCTGATCGTGGTGGATATGCA
AAACGCTTATGCCACGCCAGGAGGCTACTTAGATCTCGCCGGGTTTGATGTCTCAACCACTCGCCCGGTCATTGCCAACA
TTCAAACCGCCGTGACCGCAGCGCGAGCGGCAGGGATGCTGATCATCTGGTTTCAAAATGGCTGGGATGAACAGTATGTC
GAAGCAGGCGGACCCGGCTCACCGAATTTTCATAAATCGAACGCCCTGAAAACCATGCGTAAGCAGCCGCAGCTGCAGGG
GAAATTGCTGGCGAAAGGCTCCTGGGATTATCAACTGGTGGATGAACTGGTGCCGCAGCCTGGCGATATTGTGCTGCCGA
AGCCGCGCTACAGCGGTTTCTTCAATACGCCGCTGGACAGCATTTTGCGCAGCCGTGGAATACGCCATCTGGTTTTCACC
GGTATCGCTACCAACGTCTGCGTCGAATCGACGCTACGCGATGGCTTTTTTCTGGAGTATTTCGGCGTGGTGCTGGAAGA
CGCAACACACCAGGCGGGGCCGGAATTTGTACAGAAAGCCGCGTTGTTCAATATCGAAACCTTTTTTGGCTGGGTCAGCG
ACGTCGAAACGTTCTGCGACGCGCTTTCTCCCACGTCCTTTGCTCGTATCGCTTAA

Upstream 100 bases:

>100_bases
CCTTCGATGATTTTCTGTCGGGAATCGAAACCTTCGGCGAGCGCATTCAACCACTGATGCAGTGCCGCGCCCATCTCCCT
GCGCTGACTCAGGAGGTGGC

Downstream 100 bases:

>100_bases
GGAGTTTAACGATGCCAAAATCCGTAATTATTCCCGCTGGCAGCAGCGCACCGCTGGCCCCCTTCGTTCCCGGCACGCTG
GCTGATGGCGTGGTGTATGT

Product: putative isochorismatase family protein, rutB

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYV
EAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFT
GIATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFCDALSPTSFARIA

Sequences:

>Translated_231_residues
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYV
EAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFT
GIATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
>Mature_231_residues
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYV
EAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFT
GIATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFCDALSPTSFARIA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily

Homologues:

Organism=Escherichia coli, GI87081820, Length=230, Percent_Identity=98.695652173913, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI87081992, Length=211, Percent_Identity=28.436018957346, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTB_ECOCB (D3QPK4)

Other databases:

- EMBL:   CP001846
- RefSeq:   YP_003498826.1
- ProteinModelPortal:   D3QPK4
- EnsemblBacteria:   EBESCT00000216691
- GeneID:   8870203
- GenomeReviews:   CP001846_GR
- KEGG:   eok:G2583_1244
- GeneTree:   EBGT00050000010121
- HAMAP:   MF_00830
- InterPro:   IPR019916
- InterPro:   IPR000868
- Gene3D:   G3DSA:3.40.50.850
- TIGRFAMs:   TIGR03614

Pfam domain/function: PF00857 Isochorismatase; SSF52499 Iscrsm_hydrolase

EC number: NA

Molecular weight: Translated: 25388; Mature: 25388

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: ACT_SITE 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTA
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
ARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLV
HHHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHH
DELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEY
HHHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCHHHHH
FGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTA
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
ARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLV
HHHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHH
DELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEY
HHHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCHHHHH
FGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA