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The map label for this gene is gph [H]

Identifier: 157158678

GI number: 157158678

Start: 3828318

End: 3829076

Strand: Reverse

Name: gph [H]

Synonym: EcE24377A_3854

Alternate gene names: 157158678

Gene position: 3829076-3828318 (Counterclockwise)

Preceding gene: 157155929

Following gene: 157159383

Centisome position: 76.89

GC content: 52.7

Gene sequence:

>759_bases
ATGAATAAGTTTGAAGATATTCGCGGCGTCGCTTTTGATCTTGATGGTACGCTGGTCGACAGTGCTCCCGGTCTTGCTGC
TGCGGTAGATATGGCGCTGTATGCGCTGGAGTTGCCCATCGCAGGTGAAGAACGCGTTATTACCTGGATTGGTAACGGCG
CAGATGTTCTGATGGAGCGCGCATTGACCTGGGCGCGTCAGGAACGTGCGACTCTGCGTAAAACAATGGGTAAACCGCCC
GTTGATGACGACATTCCGGCAGAAGAACAGGTACGTATTCTGCGTAAACTGTTCGATCGCTACTATAGCGAGGTTGCCGA
AGAGGGGACGTTTTTGTTCCCGCACGTTGCCGATACGTTGGGCGCGTTGCAGGCTAAAGGCCTGCCGCTAGGCCTGGTCA
CCAACAAACCGACGCCGTTCGTCGCGCCGCTGCTCGAAGCCTTAGATATTGCCAAATACTTTAGCGTGGTTATCGGCGGC
GATGATGTGCAAAACAAAAAACCGCATCCGGACCCGCTGTTACTGGTGGCTGAGCGGATGGGAATTGCCCCACAAGAGAT
GCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGTTGCCCATCAGTTGGCTTAACCTACGGAT
ATAACTACGGCGAGGCTATCGATCTCAGCCAGCCTGATGTAATTTATCAGTCTATAAATGACCTTCTGCCCGCATTAGGG
CTTCCGCATAGCGAAAATCAGGAATCGAAAAATGACTAA

Upstream 100 bases:

>100_bases
GCGGGCGCGGATATGTTCGTCGCCGGTTCGGCAATCTTCGACCAGCCAGACTACAAAAAAGTCATTGATGAAATGCGCAG
TGAACTGGCAAAGGTAAGTC

Downstream 100 bases:

>100_bases
GCCCATCGTTTTTAGTGGCGCACAGCCCTCAGGTGAATTGACCATTGGTAACTACATGGGTGCGCTGCGTCAGTGGGTAA
ACATGCAGGATGACTACCAT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEERVITWIGNGADVLMERALTWARQERATLRKTMGKPP
VDDDIPAEEQVRILRKLFDRYYSEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
DDVQNKKPHPDPLLLVAERMGIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
LPHSENQESKND

Sequences:

>Translated_252_residues
MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEERVITWIGNGADVLMERALTWARQERATLRKTMGKPP
VDDDIPAEEQVRILRKLFDRYYSEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
DDVQNKKPHPDPLLLVAERMGIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
LPHSENQESKND
>Mature_252_residues
MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEERVITWIGNGADVLMERALTWARQERATLRKTMGKPP
VDDDIPAEEQVRILRKLFDRYYSEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
DDVQNKKPHPDPLLLVAERMGIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
LPHSENQESKND

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=252, Percent_Identity=98.4126984126984, Blast_Score=506, Evalue=1e-145,
Organism=Escherichia coli, GI1787576, Length=211, Percent_Identity=23.696682464455, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 27420; Mature: 27420

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEERVITWIGNGADVLMER
CCCHHHHCCEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
ALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYYSEVAEEGTFLFPHVADTL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHH
GALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGGDDVQNKKPHPDPLLLVAERM
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
GIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
CCCHHHEEEECCCCCHHHHHHHCCCCCCCCEECCCCCCEECCCCCHHHHHHHHHHHHHHC
LPHSENQESKND
CCCCCCCCCCCC
>Mature Secondary Structure
MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEERVITWIGNGADVLMER
CCCHHHHCCEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
ALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYYSEVAEEGTFLFPHVADTL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHH
GALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGGDDVQNKKPHPDPLLLVAERM
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
GIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
CCCHHHEEEECCCCCHHHHHHHCCCCCCCCEECCCCCCEECCCCCHHHHHHHHHHHHHHC
LPHSENQESKND
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]