| Definition | Escherichia coli E24377A, complete genome. |
|---|---|
| Accession | NC_009801 |
| Length | 4,979,619 |
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The map label for this gene is pgaA [H]
Identifier: 157158435
GI number: 157158435
Start: 1167513
End: 1169936
Strand: Reverse
Name: pgaA [H]
Synonym: EcE24377A_1144
Alternate gene names: 157158435
Gene position: 1169936-1167513 (Counterclockwise)
Preceding gene: 157159400
Following gene: 157157853
Centisome position: 23.49
GC content: 47.07
Gene sequence:
>2424_bases ATGTATTCAAGTAGCAGAAAAAGGTGCCCGAAAACCAAATGGGCTTTGAAAATTCTTACTGCCGCATTTTTAGCAGCGAG TCCCGCGGCGAAGAGTGCCGTTAATAACGCCTATGATGCATTGATTATTGAAGCTCGCAAGGGAAATACTCAGCCAGCTT TGTTATGGTTTGCACAAAAATCAGCACTCAGTAATAACCAAATTGCTGACTGGTTACAGATTGCATTATGGGCCGGGCAA GATAAACAGGTTATTACCGTTTACAACCGCTACCGTTATCAGCAATTACCAGCGCGTGGTTATGCAGCTGTCGCCGTCGC TTATCGTAACCTGCAACAATGGCAAAACTCGCTTACACTGTGGCAAAAGGCGCTCTCTCTGGAGCCGCAAAATAAGGATT ATCAACGGGGACAAATTTTAACCCTGGCAGATGCGGGTCACTATGATACTGCGCTGGTTAAACTTAAGCAGCTTAACTCT GGAGCACCGGACAAAGCCAATTTACTCGCAGAAGTCTATATCTATAAACTGGCGGGGCGTCATCAGGATGAATTACGGGC GATGACAGAGTCATTACCTGAAAATGCATCTACGCAACAATATCCCACAGAATACGTGCAGGCATTACGTAATAATCAAC TTGCTGCCGCGATTGACGATGCCAATTTAACGCCAGATATTCGCGCTGATATTCATGCCGAACTGGTCAGACTGTCGTTT ATGCCTACGCGCAGTGAAAGTGAACGTTATGCCATTGCCGATCGCGCCCTCGCCCAATACGCTGCATTAGAAATTCTGTG GCACGATAACCCAGACCGTACTGCCCAGTACCAGCGTATTCAGGTTGATCATCTTGGCGCGTTATTAACTCGCGATCGTT ATAAAGACGTTATTTCTCACTATCAGCGATTAAAAAAGACGGGGCAAATTATTCCGCCCTGGGGGCAATATTGGGTTGCA TCGGCTTATCTCAAAGATCATCAGCCGAAAAAAGCACAGTCAATAATGACTGAGCTCTTTTATCACAAGGAGACCATTGC CCCGGATTTATCCGATGAAGAACTTGCGGATCTCTTTTACAGCCACCTGGAGAGTGAAAATTATCCGGGCGCGCTAACTG TCACCCAACATACCATTAATACTTCGCCGCCTTTCCTTCGGTTAATGGGCACGCCTACGAGCATCCCGAATGATACCTGG TTACAGGGGCATTCGTTTCTCTCAACCGTAGCAAAATATAGTAATGATCTTCCTCAGGCTGAAATGACAGCCAGAGAGCT TGCTTATAACGCACCAGGAAATCAGGGACTGCGCATTGATTACGCGAGTGTGTTACAAGCCCGCGGTTGGCCTCGTGCAG CAGAAAATGAATTAAAAAAAGCAGAAGTGATCGAGCCACGTAATATTAATCTGGAGGTTGAACAAACCTGGACAGCATTA ACGTTACAAGAATGGCAGCAGGCAGCTGTCTTAACGCACGATGTTGTCGAACGTGAACCGCAAGATCCCGGCGTTGTACG ATTAAAACGTGCGGTTGATGTACATAATCTTGCAGAGCTTCGTATCGCTGGCTCAACAGGAATTGATGCCGAAGGCCCGG ATAGTGGTAAACATGATGTCGACTTAACCACCATCGTTTATTCACCACCGCTGAAGGATAACTGGCGCGGTTTTGCTGGA TTCGGTTATGCCGATGGACAATTTAGCGAAGGAAAAGGGATTGTTCGCGACTGGCTTGCGGGTGTTGAGTGGCGGTCACG TAATATCTGGCTCGAGGCAGAGTACGCTGAACGCGTTTTCAATCATGAGCATAAACCCGGCGCGCGCCTGTCTGGCTGGT ATGATTTTAATGATAACTGGCGTATTGGTTCGCAACTGGAACGCCTCTCTCACCGCGTTCCATTACGGGCAATGAAAAAT GGTGTTACAGGCAACAGTGCTCAGGCTTATGTTCGCTGGTATCAAAATGAGCGGCGTAAGTACGGTGTCTCCTGGGCTTT CACTGATTTTTCCGACAGTAACCAGCGTCATGAAGTCTCACTTGAGGGTCAGGAACGCATCTGGTCTTCACCATATTTGA TTGTCGATTTCCTACCCAGTCTGTATTACGAACAAAATACAGAACACGATACCCCATACTACAACCCTATAAAAACGTTC GATATTGTTCCGGCATTTGAGGCAAGCCATTTGTTATGGCGAAGCTATGAAAATAGCTGGGAGCAAATATTCAGCGCAGG TGTTGGTGCCTCCTGGCAAAAACATTATGGCACGGATGTCGTCACCCAACTCGGCTACGGGCAACGCATTAGCTGGAATG ACGTGATTGATGCTGGCGCAACGCTACGCTGGGAAAAACGACCTTATGACGGTGACAGAGAACACAACTTATACGTTGAA TTCGATATGACATTCAGATTTTAA
Upstream 100 bases:
>100_bases GCGTTTAATAACGGATTATGAGGTGCAAAAATATCTTTCTTTTCAGTTACCTGTAATTAGATACAGAGAGAGATTTTGGC GATACATGGAGTAATACAGG
Downstream 100 bases:
>100_bases GGATAAATATGTTACGTAATGGAAATAAATATCTCCTGATGCTGGTGAGTATAATTATGCTCACCGCGTGCATTAGCCAG TCAAGAACATCATTTATACC
Product: outer membrane protein PgaA
Products: NA
Alternate protein names: PGA export protein; Poly-beta-1,6-GlcNAc export protein [H]
Number of amino acids: Translated: 807; Mature: 807
Protein sequence:
>807_residues MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQ DKQVITVYNRYRYQQLPARGYAAVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS GAPDKANLLAEVYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVA SAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTW LQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQTWTAL TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAG FGYADGQFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKN GVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVE FDMTFRF
Sequences:
>Translated_807_residues MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQ DKQVITVYNRYRYQQLPARGYAAVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS GAPDKANLLAEVYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVA SAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTW LQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQTWTAL TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAG FGYADGQFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKN GVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVE FDMTFRF >Mature_807_residues MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQ DKQVITVYNRYRYQQLPARGYAAVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS GAPDKANLLAEVYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVA SAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTW LQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQTWTAL TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAG FGYADGQFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKN GVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVE FDMTFRF
Specific function: Exports the biofilm adhesin polysaccharide poly-beta- 1,6-N-acetyl-D-glucosamine (PGA) across the outer membrane. The PGA transported seems to be partially N-deacetylated since N- deacetylation of PGA by PgaB is needed for PGA export through the PgaA pori
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cell outer membrane; Multi-pass membrane protein [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 3 TPR repeats [H]
Homologues:
Organism=Escherichia coli, GI1787261, Length=807, Percent_Identity=99.2565055762082, Blast_Score=1654, Evalue=0.0,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013026 - InterPro: IPR011990 - InterPro: IPR019734 [H]
Pfam domain/function: NA
EC number: NA
Molecular weight: Translated: 92333; Mature: 92333
Theoretical pI: Translated: 6.52; Mature: 6.52
Prosite motif: PS50005 TPR ; PS50293 TPR_REGION
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 1.0 %Met (Translated Protein) 1.1 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 1.0 %Met (Mature Protein) 1.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALLWFAQK CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEH SALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPARGYAAVAVAYRNLQQWQNSLTL HHCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH WQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNSGAPDKANLLAEVYIYKLAGR HHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC HQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHEE MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISH CCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHH YQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFY HHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH SHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTWLQGHSFLSTVAKYSNDLPQA HHHCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCHH EMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQTWTAL HHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHH TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDV HHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCHHCCEEEEEECCCCCCCCCCCCCCCCE DLTTIVYSPPLKDNWRGFAGFGYADGQFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVF EEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCEEEEEEHHHHHHH NHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRK CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHH YGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF CCCEEEEECCCCCCCCEEEEECHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCE DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGA ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHCCCCCCCCHHHHHCCCC TLRWEKRPYDGDREHNLYVEFDMTFRF EEEECCCCCCCCCCCEEEEEEEEEECC >Mature Secondary Structure MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALLWFAQK CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEH SALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPARGYAAVAVAYRNLQQWQNSLTL HHCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH WQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNSGAPDKANLLAEVYIYKLAGR HHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC HQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHEE MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISH CCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHH YQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFY HHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH SHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTWLQGHSFLSTVAKYSNDLPQA HHHCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCHH EMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQTWTAL HHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHH TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDV HHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCHHCCEEEEEECCCCCCCCCCCCCCCCE DLTTIVYSPPLKDNWRGFAGFGYADGQFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVF EEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCEEEEEEHHHHHHH NHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRK CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHH YGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF CCCEEEEECCCCCCCCEEEEECHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCE DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGA ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHCCCCCCCCHHHHHCCCC TLRWEKRPYDGDREHNLYVEFDMTFRF EEEECCCCCCCCCCCEEEEEEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]