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The map label for this gene is frvB

Identifier: 157158434

GI number: 157158434

Start: 4418797

End: 4420248

Strand: Reverse

Name: frvB

Synonym: EcE24377A_4429

Alternate gene names: 157158434

Gene position: 4420248-4418797 (Counterclockwise)

Preceding gene: 157157981

Following gene: 157157925

Centisome position: 88.77

GC content: 55.58

Gene sequence:

>1452_bases
ATGGAGTCATCCTTACGTATTGTCGCGATCACCAACTGCCCCGCCGGGATCGCTCACACCTACATGGTGGCGGAAGCCCT
GGAACAGAAAGCCCGTTCTCTCGGCCATACCATAAAAGTGGAAACTCAAGGGTCCAGTGGCGTTGAAAACCGCTTATCCA
GCGAAGAGATTGCCGCTGCCGATTACGTCATTCTCGCTACCGGGCGTGGCCTGAGCGGTGATGATCGCGCGCGGTTTGCC
GGGAAGAAAGTTTATGAGATTGCCATCTCCCAGGCGTTGAAAAATATCGACCAGATTTTCAGCGAATTACCGACAAACTC
GCAGCTTTTTGCCGCAGATAGCGGCGTGAAGCTTGGCAAACAGGAAGTGCAAAGCGGCAGCGTGATGAGCCACCTGATGG
CGGGCGTCTCTGCGGCGCTGCCGTTTGTCATCGGCGGTGGTATCCTGGTGGCGCTTGCCAACATGCTGGTGCAGTTCGGT
TTGCCGTATACCGATATGTCGAAAGGCGCGCCGTCGTTTACCTGGGTAGTTGAATCCATCGGTTACCTTGGCTTCACCTT
TATGATCCCCATCATGGGCGCTTATATTGCCTCGTCGATTGCCGATAAACCGGCTTTCGCTCCGGCGTTTCTGGTTTGCT
ATCTGGCGAACGACAAAGCGCTGCTCGGCACCCAGTCGGGTGCGGGATTCCTCGGCGCGGTGGTGCTGGGGCTGGCGATT
GGCTATTTCGTCTTCTGGTTCCGCAAAGTGCGCCTTGGCAAAGCATTACAACCGCTGCTCGGTTCAATGCTGATCCCGTT
CGTTACTCTGCTGGTTTTCGGCGTGCTCACTTACTACGTTATCGGGCCGGTGATGTCCGACCTCATGGGCGGGCTGCTCC
ACTTCCTGAATACCATTCCTCCATCAATGAAGTTTGCGGCAGCGTTTCTGGTCGGTGCGATGCTGGCGTTCGATATGGGC
GGCCCCATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGG
CGTTGTCGCGTTGATGCCGCCCGTTGCGGCGGGTCTTGCGACGTTCATTGCGCCCAAACTGTTTACCCGACAGGAAAAAG
AGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACCGAACCTGCTATTCCTTACGCTCTGGCAGCTCCGTTA
CCGATGATTACCGCCAATACGCTGGCGGGCGGTATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACC
TGGTTTGGGTATCTTTGACCCGCTGATCGGCCTGATGTCGCCGGTAGGTTCGTTTTATCTGGTGCTGGCGATTGGCCTCG
CGCTAAACATTTCATTCATTATCGTCCTGAAAGGATTGTGGTTACGCCGCAAAGCGAAAGCCGCGCAACAGGAGCTTGTC
CATGAACATTGA

Upstream 100 bases:

>100_bases
AAAATTATCCACCAGGATTTTATTCATCAACTGCAACAGGGCGACGCCGACCAGGTGCTTGCCTTGTTAAATCAAACCCT
CAGCTCATAAGGAAGTGGCG

Downstream 100 bases:

>100_bases
GTTACTGCAACAGTTGTGCGAAGCCAGCGCCGTCAGCGGCGATGAACAGGAAGTTCGCGACATCCTGATAAACACGCTGG
AACCTTGCGTTAATGAAATC

Product: putative PTS system transporter subunits IIBC

Products: NA

Alternate protein names: Fructose-like phosphotransferase enzyme IIB component; PTS system fructose-like EIIB component; Fructose-like permease IIC component; PTS system fructose-like EIIC component

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIAAADYVILATGRGLSGDDRARFA
GKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGLAI
GYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMG
GPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYALAAPL
PMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELV
HEH

Sequences:

>Translated_483_residues
MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIAAADYVILATGRGLSGDDRARFA
GKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGLAI
GYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMG
GPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYALAAPL
PMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELV
HEH
>Mature_483_residues
MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIAAADYVILATGRGLSGDDRARFA
GKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGLAI
GYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMG
GPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYALAAPL
PMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELV
HEH

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain

Homologues:

Organism=Escherichia coli, GI87082348, Length=483, Percent_Identity=100, Blast_Score=965, Evalue=0.0,
Organism=Escherichia coli, GI1788492, Length=444, Percent_Identity=34.009009009009, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1786951, Length=479, Percent_Identity=32.5678496868476, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1790386, Length=291, Percent_Identity=35.7388316151203, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1790387, Length=98, Percent_Identity=50, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788729, Length=322, Percent_Identity=27.3291925465839, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788730, Length=100, Percent_Identity=40, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1790390, Length=70, Percent_Identity=45.7142857142857, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTFLB_ECOLI (P32154)

Other databases:

- EMBL:   L19201
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_003909.1
- RefSeq:   NP_418335.4
- ProteinModelPortal:   P32154
- SMR:   P32154
- STRING:   P32154
- EnsemblBacteria:   EBESCT00000002508
- EnsemblBacteria:   EBESCT00000015727
- GeneID:   948390
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5562
- KEGG:   eco:b3899
- EchoBASE:   EB1809
- EcoGene:   EG11863
- eggNOG:   COG1299
- GeneTree:   EBGT00070000031702
- HOGENOM:   HBG704192
- OMA:   YEVHVET
- ProtClustDB:   PRK11404
- BioCyc:   EcoCyc:FRVB-MONOMER
- Genevestigator:   P32154
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327
- TIGRFAMs:   TIGR00829
- TIGRFAMs:   TIGR01427

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB

EC number: =2.7.1.69

Molecular weight: Translated: 51121; Mature: 51121

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS51099 PTS_EIIB_TYPE_2; PS51104 PTS_EIIC_TYPE_2

Important sites: ACT_SITE 13-13

Signals:

None

Transmembrane regions:

HASH(0x2203c658)-; HASH(0x21716db8)-; HASH(0x221606f8)-; HASH(0x21f614c4)-; HASH(0x2181b9f4)-; HASH(0x22216f94)-; HASH(0x21328dd4)-; HASH(0x22540de0)-; HASH(0x219b83bc)-; HASH(0x225ba424)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIAAA
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCHHHHHHH
DYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGK
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHCH
QEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFGLPYTDMSKGAPSFTWVVESI
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
GYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGLAI
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEECCCCEEEECCCCCHHHHHHHHHHHH
GYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLA
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAF
HHHHHHHHHHHHHHHHCCEEEEEEEHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
GIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEH
CCH
>Mature Secondary Structure
MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIAAA
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCHHHHHHH
DYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGK
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHCH
QEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFGLPYTDMSKGAPSFTWVVESI
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
GYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGLAI
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEECCCCEEEECCCCCHHHHHHHHHHHH
GYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLA
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAF
HHHHHHHHHHHHHHHHCCEEEEEEEHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
GIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEH
CCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8346018; 9278503; 8019415