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The map label for this gene is recE [H]

Identifier: 157158330

GI number: 157158330

Start: 1263530

End: 1264297

Strand: Reverse

Name: recE [H]

Synonym: EcE24377A_1250

Alternate gene names: 157158330

Gene position: 1264297-1263530 (Counterclockwise)

Preceding gene: 157156645

Following gene: 157155362

Centisome position: 25.39

GC content: 48.83

Gene sequence:

>768_bases
ATGGGCGACGCAACATACCAGGAAACATTCAATGAAGAGAATCAGGTTGAAGTTCAGGAAAATGATCCGGAGGAAATGGA
AGGCGCTGAACATCCACACAAGGAGAATGCTGACAGGTATCAGGACCACGCCAGCGATAATGAAACTGGCGAGGCGACAG
CTCCCTTAATTAATGTGAACGGTCATCACGAAATCACATCCACCAGCAGAGTGTGGCACCATCTGATGATCGACCTTGAA
ACGATGGGAAAAAATCCTGATGCGCCAATCAACTCTATTGGCGGTAAGTTTTTTGATCCAGTAACCGGAGAGATGGGGCC
GGAATTTAGCAAGACCATCGATCTGGAAACAGCTGGCGGAGTCATTGATCGTGACACCATTAAATGGTGGCTTAAGCAAT
CACGCGAGGCGCAATCTGCCATTCTGACCGATGAAATCCCGTTAGATGATGCACTACTGCAATTGCGGGAATTTATCGCC
GAAAACTCCGGTGAATTTTTTGTTCAGATCTGGGGAAATGGAGCCAACTTCGACAACACGATTTTGCGCCGTTCATACGA
ACGGCAGGGGATCCCCTGCCCGTGGCGTTACTACAACGATCGCGATGTACGCACAATCGTTGAGCTGGGGAAAGCCATAG
GCTTCGATGCCAGAACGGCTATTCCATTCGAAGGTGAGCGCCATAATGCACTTGATGACGCTCGTCACCAGGCAAAATAC
GTTTCAGCTATCTGGCAAAAACTGATCCCGAGTCAGGCTGATTTTTAA

Upstream 100 bases:

>100_bases
CCCGCGATTCTGTGAACCAGTAGCGCAACAGGAACCGGAAAAGATCTGCACCGCCTGCGGTCAGAGCGATGGCGGCAACT
GCCCTGATTGTGGCGCGGTG

Downstream 100 bases:

>100_bases
TGTTCAACCGTCGCCAGTTGTCGTTGGTATTCTGCAACTGGCGCGTTCCGGAGTGATAGCCATGAGCGAACAGTACCTGA
TAACGCTCGACGAGTGGAAA

Product: exonuclease family protein

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MGDATYQETFNEENQVEVQENDPEEMEGAEHPHKENADRYQDHASDNETGEATAPLINVNGHHEITSTSRVWHHLMIDLE
TMGKNPDAPINSIGGKFFDPVTGEMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAILTDEIPLDDALLQLREFIA
ENSGEFFVQIWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIGFDARTAIPFEGERHNALDDARHQAKY
VSAIWQKLIPSQADF

Sequences:

>Translated_255_residues
MGDATYQETFNEENQVEVQENDPEEMEGAEHPHKENADRYQDHASDNETGEATAPLINVNGHHEITSTSRVWHHLMIDLE
TMGKNPDAPINSIGGKFFDPVTGEMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAILTDEIPLDDALLQLREFIA
ENSGEFFVQIWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIGFDARTAIPFEGERHNALDDARHQAKY
VSAIWQKLIPSQADF
>Mature_254_residues
GDATYQETFNEENQVEVQENDPEEMEGAEHPHKENADRYQDHASDNETGEATAPLINVNGHHEITSTSRVWHHLMIDLET
MGKNPDAPINSIGGKFFDPVTGEMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAILTDEIPLDDALLQLREFIAE
NSGEFFVQIWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIGFDARTAIPFEGERHNALDDARHQAKYV
SAIWQKLIPSQADF

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=57, Percent_Identity=66.6666666666667, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 28975; Mature: 28844

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDATYQETFNEENQVEVQENDPEEMEGAEHPHKENADRYQDHASDNETGEATAPLINVN
CCCCHHHHHCCCCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECC
GHHEITSTSRVWHHLMIDLETMGKNPDAPINSIGGKFFDPVTGEMGPEFSKTIDLETAGG
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCHHHEECCCCCCC
VIDRDTIKWWLKQSREAQSAILTDEIPLDDALLQLREFIAENSGEFFVQIWGNGANFDNT
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHH
ILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIGFDARTAIPFEGERHNALDDARHQAKY
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
VSAIWQKLIPSQADF
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
GDATYQETFNEENQVEVQENDPEEMEGAEHPHKENADRYQDHASDNETGEATAPLINVN
CCCHHHHHCCCCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECC
GHHEITSTSRVWHHLMIDLETMGKNPDAPINSIGGKFFDPVTGEMGPEFSKTIDLETAGG
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCHHHEECCCCCCC
VIDRDTIKWWLKQSREAQSAILTDEIPLDDALLQLREFIAENSGEFFVQIWGNGANFDNT
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHH
ILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIGFDARTAIPFEGERHNALDDARHQAKY
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
VSAIWQKLIPSQADF
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]