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The map label for this gene is menE [H]

Identifier: 157158327

GI number: 157158327

Start: 2548505

End: 2549860

Strand: Reverse

Name: menE [H]

Synonym: EcE24377A_2556

Alternate gene names: 157158327

Gene position: 2549860-2548505 (Counterclockwise)

Preceding gene: 157158055

Following gene: 157157275

Centisome position: 51.21

GC content: 56.42

Gene sequence:

>1356_bases
ATGATCTTCTCTGACTGGCCGTGGCGTCACTGGCGGCAAGTGCGGGGAGAAGCCATCGCCTTACGTCTTAATGACGAGCA
ACTTAACTGGCGCGAGCTTTGTGCTCGCGTCGATGAATTAGCCTCAGGCTTTGCGGCGCAGGGCGTTGTTGAGGGCAGCG
GCGTGATGTTGCGGGCGTGGAATACGCCGCAAACGCAGCTCGCCTGGCTGGCGTTACTGCAATGTGGGGCGCGGGTGTTG
CCCGTGAACCCTCAGCTGCCGCAACCGTTGCTTGAAGAATTGCTGCCCAATCTGACGCTGCAATTTGCGCTGGTGCCGGA
TGGGGAAAACACGTTTCCGGCGTTAACGTCGCTGCACATTCAGCGGGTTGAAGGCGCACATGCCGCTACGTGGCAGCCGA
CGCGTCTGTGCTCAATGACGTTGACCTCGGGTTCTACCGGGTTGCCGAAAGCCGCTGTCCATACTTATCAGGCCCATCTT
GCCAGTGCCGAAGGGGTGTTATCGTTGATTCCGTTTGGCGATCACGATGACTGGTTGCTCTCTTTACCGCTGTTTCACGT
CTCCGGTCAGGGAATTATGTGGCGCTGGTTATACGCTGGTGCGCGGATGACGGTGCGTGATAAACAGCCATTGGAGCAAA
TGCTGGCAGGCTGTACTCACGCTTCACTGGTGCCAACACAACTCTGGCGTTTGCTGGTTAACCGCAGTTCCGTTTCCCTG
AAAGCGGTGTTACTTGGCGGCGCGGCTATCCCGGTCGAGTTGACGGAACAGGCGCGCGAGCAGGGGATTCGTTGCTTTTG
CGGCTATGGTCTGACCGAGTTTGCCTCCACGGTGTGTGCGAAAGAAGCCGACGGCCTGGCAGACGTTGGTTCGCCGCTGC
CGGGTCGGGAAGTGAAAATCGTTAATAATGAAGTGTGGCTGCGGGCTGCCAGTATGGCAGAAGGTTACTGGCGTAACGGG
CAACTGGTTTCACTGGTTAATGACGAAGGCTGGTACGCTACGCGCGATCGCGGTGAGATGCATAATGGCAAGCTGACCAT
TGTCGGACGTTTAGACAATCTGTTCTTCAGTGGCGGTGAGGGTATTCAGCCGGAAGAAGTCGAGCGTGTTATTGCTGCAC
ATCCTGCGGTTTTGCAGGTGTTTATCGTTCCCGTTGCCGACAAGGAGTTTGGTCATCGGCCGGTAGCGGTGGTGGAGTAT
GACCAGCAAACCGTTGATCTTGGCGAATGGGTGAAAGATAAGCTGGCTCGTTTTCAACAACCGGTGCGCTGGCTGACTTT
ACCGCCGGAGCTGAAAAACGGCGGTATTAAAATTTCACGTCAGGCGCTAAAAGAGTGGGTGCAACGTCAGCAATAA

Upstream 100 bases:

>100_bases
CAGGGCTGGACACGCTGGATCTGATGCAGGCGCAGCAGGTACGTCGCTGGCCGGGTAGCACGCTGCCTGTCGTGGAAGTT
GATGCACTGGAGCGGTTGTT

Downstream 100 bases:

>100_bases
TTAACCCTCCGTGTCACTTCTCATTTCTGCCATTCCATTGCCGTTGATAAAGAGTTTATCTGTTAATCTGTAAATTGATG
TGAAAACTCTTAGCAAACAG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAAQGVVEGSGVMLRAWNTPQTQLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNG
QLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ

Sequences:

>Translated_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAAQGVVEGSGVMLRAWNTPQTQLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNG
QLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ
>Mature_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAAQGVVEGSGVMLRAWNTPQTQLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNG
QLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=451, Percent_Identity=97.7827050997783, Blast_Score=862, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=378, Percent_Identity=24.8677248677249, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI221142682, Length=494, Percent_Identity=23.4817813765182, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786810, Length=506, Percent_Identity=23.7154150197628, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788107, Length=352, Percent_Identity=26.4204545454545, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789201, Length=364, Percent_Identity=25.2747252747253, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI32563687, Length=459, Percent_Identity=24.6187363834423, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=483, Percent_Identity=25.6728778467909, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=499, Percent_Identity=24.4488977955912, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994694, Length=355, Percent_Identity=27.3239436619718, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=355, Percent_Identity=27.0422535211268, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=355, Percent_Identity=27.0422535211268, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25141359, Length=399, Percent_Identity=23.8095238095238, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17557194, Length=363, Percent_Identity=24.7933884297521, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=295, Percent_Identity=26.1016949152542, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17538037, Length=357, Percent_Identity=23.8095238095238, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581924, Length=531, Percent_Identity=22.7871939736347, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50128; Mature: 50128

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAAQGVVEGSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHCCEEECCCEEEEEC
NTPQTQLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHI
CCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
QRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAEGVLSLIPFGDHDDWLL
HHCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCEEEE
VNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGE
ECCCEEEEEHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCCCEEEEEEEECCEEECCCC
GIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEYDQQTVDLGEWVKDKLARFQQ
CCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ
CCEEEECCHHHCCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAAQGVVEGSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHCCEEECCCEEEEEC
NTPQTQLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHI
CCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
QRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAEGVLSLIPFGDHDDWLL
HHCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCEEEE
VNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGE
ECCCEEEEEHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCCCEEEEEEEECCEEECCCC
GIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEYDQQTVDLGEWVKDKLARFQQ
CCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ
CCEEEECCHHHCCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626063; 9205837; 9278503 [H]