Click here to switch to the map view.

The map label for this gene is cspB [H]

Identifier: 157158051

GI number: 157158051

Start: 1762391

End: 1762606

Strand: Reverse

Name: cspB [H]

Synonym: EcE24377A_1765

Alternate gene names: 157158051

Gene position: 1762606-1762391 (Counterclockwise)

Preceding gene: 157157501

Following gene: 157157405

Centisome position: 35.4

GC content: 34.72

Gene sequence:

>216_bases
ATGTCAAATAAAATGACTGGTTTAGTAAAATGGTTTAACGCTGATAAAGGTTTCGGCTTTATTTCTCCTGTTGATGGTAG
TAAAGATGTGTTTGTGCATTTTTCTGCGATTCAGAATGATAATTATCGAACCTTATTTGAAGGTCAAAAGGTTACCTTCT
CTATAGAGAGTGGTGCTAAAGGTCCTGCAGCAGTAAATGTCATCATTACTGATTAA

Upstream 100 bases:

>100_bases
TTATTCACTTATTCCTTCTTTGAGTCTCTCCAATTAAGTACGAAGTCGTTTCTGTTATGCAAACCATTTATGCCGAAAGG
CTCAAGTTAAGGAATGTAGA

Downstream 100 bases:

>100_bases
AATTCATCGCTCGTCTGTATACGATAACGAAGAAGGCTGATGCCTGAGTAGAGATACGGACAGAGTAGTGAATATTGGAT
CTCTTTAATAAAAAGTAAGG

Product: cold shock DNA-binding protein

Products: NA

Alternate protein names: CSP-B [H]

Number of amino acids: Translated: 71; Mature: 70

Protein sequence:

>71_residues
MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRTLFEGQKVTFSIESGAKGPAAVNVIITD

Sequences:

>Translated_71_residues
MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRTLFEGQKVTFSIESGAKGPAAVNVIITD
>Mature_70_residues
SNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRTLFEGQKVTFSIESGAKGPAAVNVIITD

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787839, Length=71, Percent_Identity=98.5915492957747, Blast_Score=144, Evalue=9e-37,
Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=83.5820895522388, Blast_Score=117, Evalue=1e-28,
Organism=Escherichia coli, GI2367114, Length=68, Percent_Identity=77.9411764705882, Blast_Score=114, Evalue=1e-27,
Organism=Escherichia coli, GI1787834, Length=68, Percent_Identity=73.5294117647059, Blast_Score=108, Evalue=6e-26,
Organism=Escherichia coli, GI1786841, Length=66, Percent_Identity=68.1818181818182, Blast_Score=96, Evalue=3e-22,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=70.3125, Blast_Score=96, Evalue=3e-22,
Organism=Escherichia coli, GI1787107, Length=62, Percent_Identity=53.2258064516129, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI2367112, Length=70, Percent_Identity=45.7142857142857, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7745; Mature: 7614

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRTLFEGQKVTFSIESGAK
CCCCCCEEEEEECCCCCCEEEECCCCCCEEEEEEEEECCCCEEEEECCCEEEEEECCCCC
GPAAVNVIITD
CCEEEEEEEEC
>Mature Secondary Structure 
SNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRTLFEGQKVTFSIESGAK
CCCCCEEEEEECCCCCCEEEECCCCCCEEEEEEEEECCCCEEEEECCCEEEEEECCCCC
GPAAVNVIITD
CCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8022261; 9439003; 9097039; 9278503 [H]