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The map label for this gene is yjjW [H]

Identifier: 157157888

GI number: 157157888

Start: 4953721

End: 4954584

Strand: Reverse

Name: yjjW [H]

Synonym: EcE24377A_4977

Alternate gene names: 157157888

Gene position: 4954584-4953721 (Counterclockwise)

Preceding gene: 157157718

Following gene: 157159050

Centisome position: 99.5

GC content: 55.21

Gene sequence:

>864_bases
ATGAACAGCAGATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTT
TTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGCTGCAATGATTGCGGGGAGTGCG
TGCCACAGTGTCCGCATCAGGCATTGCAGATTGTTGACGGCAACGTACTGTGGAACGCTGTGGTTTGCGAGCAGTGTGAT
ACCTGCCTGAAGATGTGTCCGCAACATGCCACGCCAATGGCGCAATCCATGAGCGTGGATGAGGTGCTTAGCCATATCCG
CAAAGCGGTGCTGTTTATTGAAGGGATAACGGTAAGCGGCGGCGAAGCCACGACCCAACTGCCGTTTGTAGTGGCGCTAT
TTACAGCCATCAAAAACGATCCGCAACTGCGCCACCTCACCTGCCTCGTGGACAGTAACGGTATGTTGAGCGAAACCGGC
TGGGAAAAATTACTCCCGGTGTGCGACGGCGCGATGCTCGATCTCAAAGCGTGGGGGAGCGAATGTCATCAGCAATTAAC
CGGACGCGATAATCAGCAGATTAAGCGCAGCATCTGTTTGCTGGCAGAGCGCGGTAAGCTGGCGGAACTGCGTTTGCTGG
TAATTCCAGACCAGGTGGATTATTTGCATCACATCGATGAGCTGGCGACGTTTATCAAGAGACTTGGCGATGTTCCGGTT
CGCCTGAATGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCCGGAAGACGTTGAGCC
GTTGGCTGATGCGTTAAAGGTGCGCGGGGTGAGCCGGTTGATATTTCCGGCGCTCTATTTGTGA

Upstream 100 bases:

>100_bases
AAATATCGCGCCGAAGGTTCACGCACCAACACCACCTGGCTGGGCGAAGAAGCCGCACGCAACACTCGTATTCTGGAACG
CCAGCCGCGCGTGATAAGCC

Downstream 100 bases:

>100_bases
CATGCCGGATACGGCCTACGGTTCGGCACAAACCTGTAGGCATGATAAGACGCGACAAGCGTCGCATCAGGCATTATGCG
CCGACTGCCGGATGCTGCCT

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQALQIVDGNVLWNAVVCEQCD
TCLKMCPQHATPMAQSMSVDEVLSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQVDYLHHIDELATFIKRLGDVPV
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL

Sequences:

>Translated_287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQALQIVDGNVLWNAVVCEQCD
TCLKMCPQHATPMAQSMSVDEVLSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQVDYLHHIDELATFIKRLGDVPV
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL
>Mature_287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQALQIVDGNVLWNAVVCEQCD
TCLKMCPQHATPMAQSMSVDEVLSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQVDYLHHIDELATFIKRLGDVPV
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790839, Length=287, Percent_Identity=96.5156794425087, Blast_Score=573, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 31602; Mature: 31602

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: PS01087 RADICAL_ACTIVATING ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
5.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQ
CCCCHHHHHHHCCCEECCCCCHHEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHCHHH
ALQIVDGNVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEVLSHIRKAVLFIEGITVSG
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEECC
GEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETGWEKLLPVCDGAMLDLKAWGS
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
ECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQVDYLHHIDELATFIKRLGDVPV
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCE
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL
EEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQ
CCCCHHHHHHHCCCEECCCCCHHEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHCHHH
ALQIVDGNVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEVLSHIRKAVLFIEGITVSG
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEECC
GEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETGWEKLLPVCDGAMLDLKAWGS
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
ECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQVDYLHHIDELATFIKRLGDVPV
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCE
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL
EEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]