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The map label for this gene is istB [H]

Identifier: 157157772

GI number: 157157772

Start: 3390926

End: 3391753

Strand: Reverse

Name: istB [H]

Synonym: EcE24377A_3369

Alternate gene names: 157157772

Gene position: 3391753-3390926 (Counterclockwise)

Preceding gene: 157155459

Following gene: 157155468

Centisome position: 68.11

GC content: 57.37

Gene sequence:

>828_bases
ATGCATGAACTGGAGACACTGCTGAGTCGTCTTAAAATGGAACATCTGGGCTACCACGTTGAAAGCCTGCTGGAGCAGGC
GGCCAAAAAAGAACTGAACTACCGCGAGTTCCTGTGCATGGCGTTGCAGCAGGAATGGAACGGGAGGCATCAGCGCGGTA
TGGAGTCCCGGCTGAAACAGGCCCGCTTCCCGTGGGTCAAAACGCTGGAGCAGTTCGACTTCGGCTTCCAGCCAGGTATC
GATCGTAAGGTCGTCCGTGAGCTGGCCGGGCTGGCGTTCGTGGAGCGTAGCGAGAACGTGATCCTGCTGGGTCCGCCCGG
TGTGGGTAAAACGCACCTGGCAGTCGCTCTCGGGGTAAAAGCGGCGGACGCTGGTCATCGGGTACTGTTCATGCCACTGG
ATAAGCTGATCGCCACACTCATGAAGGCAAAACAGGAAAACCGGCTGGAAAAACAGCTACAGCAGCTTGGTTATGCCCGG
GTATACGGTGTGGAGTGGCCATCAGGTCACAATGTTCGCTGGGTGCGTGATGAGATGAGCAGTCTGGTGCTGCTGACTGA
CACACCCTGGTATCCGCCTTCAGGTGAGCTGGTTGGTGAAATCTCGAAGGTATTCGACTGTGAGATACGGCACTGGTACA
GCGAACCAGTCCGGGGCATACAGGGTTACAACTGTTATGACGGTGGTGAACATACGGACAGTGACCCGCAGGCCGAATGG
CCAGGCAGGGAAACACTTCCTCAGCCCCGGCTGTATCTGGTGGAGGAGCGCGCTGAAGAACAGACAGAAGCCGCGCCTTT
ACCGGTTCCGCTGGCTTCAGGCCAGTAA

Upstream 100 bases:

>100_bases
CATCCAGCTGGCAAACCGTGCCGGAGCATCACGCCCCACTCTGGCAGCAGGTCAGTCAGGTGGAACACAGACCGTTGAGT
ACGTATGAGGAGCTGTTGTG

Downstream 100 bases:

>100_bases
TTAACCTCAGGGTGCCACATCGTGGCACCCGTTTTTTCCTGTCATCTTTCATTTCAGCCCGAAGGGGAACACTTGTTCCT
CCCCGGGACCATGTGTTCCC

Product: IS21 family transposition helper protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MHELETLLSRLKMEHLGYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQARFPWVKTLEQFDFGFQPGI
DRKVVRELAGLAFVERSENVILLGPPGVGKTHLAVALGVKAADAGHRVLFMPLDKLIATLMKAKQENRLEKQLQQLGYAR
VYGVEWPSGHNVRWVRDEMSSLVLLTDTPWYPPSGELVGEISKVFDCEIRHWYSEPVRGIQGYNCYDGGEHTDSDPQAEW
PGRETLPQPRLYLVEERAEEQTEAAPLPVPLASGQ

Sequences:

>Translated_275_residues
MHELETLLSRLKMEHLGYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQARFPWVKTLEQFDFGFQPGI
DRKVVRELAGLAFVERSENVILLGPPGVGKTHLAVALGVKAADAGHRVLFMPLDKLIATLMKAKQENRLEKQLQQLGYAR
VYGVEWPSGHNVRWVRDEMSSLVLLTDTPWYPPSGELVGEISKVFDCEIRHWYSEPVRGIQGYNCYDGGEHTDSDPQAEW
PGRETLPQPRLYLVEERAEEQTEAAPLPVPLASGQ
>Mature_275_residues
MHELETLLSRLKMEHLGYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQARFPWVKTLEQFDFGFQPGI
DRKVVRELAGLAFVERSENVILLGPPGVGKTHLAVALGVKAADAGHRVLFMPLDKLIATLMKAKQENRLEKQLQQLGYAR
VYGVEWPSGHNVRWVRDEMSSLVLLTDTPWYPPSGELVGEISKVFDCEIRHWYSEPVRGIQGYNCYDGGEHTDSDPQAEW
PGRETLPQPRLYLVEERAEEQTEAAPLPVPLASGQ

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 31414; Mature: 31414

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHELETLLSRLKMEHLGYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
ARFPWVKTLEQFDFGFQPGIDRKVVRELAGLAFVERSENVILLGPPGVGKTHLAVALGVK
HCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHCCCE
AADAGHRVLFMPLDKLIATLMKAKQENRLEKQLQQLGYARVYGVEWPSGHNVRWVRDEMS
ECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEHHHHC
SLVLLTDTPWYPPSGELVGEISKVFDCEIRHWYSEPVRGIQGYNCYDGGEHTDSDPQAEW
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PGRETLPQPRLYLVEERAEEQTEAAPLPVPLASGQ
CCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MHELETLLSRLKMEHLGYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
ARFPWVKTLEQFDFGFQPGIDRKVVRELAGLAFVERSENVILLGPPGVGKTHLAVALGVK
HCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHCCCE
AADAGHRVLFMPLDKLIATLMKAKQENRLEKQLQQLGYARVYGVEWPSGHNVRWVRDEMS
ECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEHHHHC
SLVLLTDTPWYPPSGELVGEISKVFDCEIRHWYSEPVRGIQGYNCYDGGEHTDSDPQAEW
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PGRETLPQPRLYLVEERAEEQTEAAPLPVPLASGQ
CCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]