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The map label for this gene is gloB

Identifier: 157157699

GI number: 157157699

Start: 236247

End: 237002

Strand: Reverse

Name: gloB

Synonym: EcE24377A_0217

Alternate gene names: 157157699

Gene position: 237002-236247 (Counterclockwise)

Preceding gene: 157157206

Following gene: 157157200

Centisome position: 4.76

GC content: 40.21

Gene sequence:

>756_bases
ATGAATCTTAACAGTATTCCCGCCTTTGATGACAATTACATCTGGGTTTTGAATGATGAAGCAGGTCGCTGCCTGATTGT
CGATCCCGGAGACGCAGAGCCAGTATTAAACGCCATTGCCGCCAATAACTGGCAACCGGAGGCCATATTTCTCACCCACC
ATCATCACGATCACGTTGGCGGCGTAAAAGAACTGGTGGAAAAGTTTCCGCAAATTGTGGTGTATGGTCCACAAGAGACA
CAAGATAAGGGAACAACACAGGTAGTCAAAGATGGCGAAACTGCCTTCGTTTTGGGGCATGAATTTAGTGTAATTGCCAC
ACCGGGTCACACTTTAGGACATATCTGTTACTTCAGTAAACCTTATCTATTTTGCGGCGACACGCTGTTTTCTGGTGGGT
GTGGTAGGTTGTTTGAAGGGACAGCATCACAAATGTATCAATCACTTAATAAGTTAAGTGCGTTACCTGACGATACATTG
GTATGTTGTGCTCATGAATATACGTTATCAAATATGAAGTTTGCTTTGAGTATTCTTCCGCACGATTTGTCCATAAATGA
TTATTATCGTAAAGTTAAGGAGTTACGGGCAAAAAATCAAATAACACTACCCGTAATTCTGAAAAATGAGCGGCAAATTA
ATGTTTTTTTAAGAACGGAAGATATTGATTTAATTAATGTAATTAATGAAGAAACATTATTGCAACAACCTGAAGAGCGT
TTTGCATGGTTAAGGTCAAAGAAAGATAGGTTCTGA

Upstream 100 bases:

>100_bases
CTCCCCAAGGCAAATCGCCCCAGCAATCAGGAGCCACGACAGTTTGAGGGACTCTTGCCGGTTTCATCACAACCTTCCGT
TTCACACTGAGAGGTAATCT

Downstream 100 bases:

>100_bases
TAAAACTTTCTTGTCATCGGCTCCGTTCGCCGTTATGATCGGTCGTCTTTTAAGCAACTATTGACACACACATGAAGGCA
AAAGCGATATTACTCGCCTC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNWQPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQET
QDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTEDIDLINVINEETLLQQPEER
FAWLRSKKDRF

Sequences:

>Translated_251_residues
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNWQPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQET
QDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTEDIDLINVINEETLLQQPEER
FAWLRSKKDRF
>Mature_251_residues
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNWQPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQET
QDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTEDIDLINVINEETLLQQPEER
FAWLRSKKDRF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=34.2307692307692, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=34.2307692307692, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI14150041, Length=229, Percent_Identity=35.3711790393013, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI116642887, Length=232, Percent_Identity=35.3448275862069, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI21703352, Length=232, Percent_Identity=35.3448275862069, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=37.3737373737374, Blast_Score=122, Evalue=3e-28,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=99.601593625498, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1787158, Length=193, Percent_Identity=29.5336787564767, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=227, Percent_Identity=33.0396475770925, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320478, Length=231, Percent_Identity=33.7662337662338, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=231, Percent_Identity=30.3030303030303, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356335, Length=261, Percent_Identity=35.632183908046, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24667711, Length=261, Percent_Identity=35.632183908046, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24667703, Length=261, Percent_Identity=35.632183908046, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_ECO24 (A7ZHU8)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461377.1
- ProteinModelPortal:   A7ZHU8
- SMR:   A7ZHU8
- STRING:   A7ZHU8
- EnsemblBacteria:   EBESCT00000021236
- GeneID:   5587003
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0217
- eggNOG:   COG0491
- GeneTree:   EBGT00050000008983
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   PRK10241
- BioCyc:   ECOL331111:ECE24377A_0217-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28421; Mature: 28421

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNWQPEAIFLTHHHHDHVG
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCC
GVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSK
HHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCEEEEEECCEEEEECCCCHHEEEEEECC
PYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTLVCCAHEYTLSNMKFALSILP
CEEEECCCHHCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCEEEEEECC
HDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTEDIDLINVINEETLLQQPEER
CCCCHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEECCCEEEECCCHHHHHCCHHHH
FAWLRSKKDRF
HHHHHHCCCCC
>Mature Secondary Structure
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNWQPEAIFLTHHHHDHVG
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCC
GVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSK
HHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCEEEEEECCEEEEECCCCHHEEEEEECC
PYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTLVCCAHEYTLSNMKFALSILP
CEEEECCCHHCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCEEEEEECC
HDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTEDIDLINVINEETLLQQPEER
CCCCHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEECCCEEEECCCHHHHHCCHHHH
FAWLRSKKDRF
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA