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The map label for this gene is pcaI [H]

Identifier: 157157645

GI number: 157157645

Start: 1665180

End: 1665887

Strand: Reverse

Name: pcaI [H]

Synonym: EcE24377A_1670

Alternate gene names: 157157645

Gene position: 1665887-1665180 (Counterclockwise)

Preceding gene: 157159094

Following gene: 157157143

Centisome position: 33.45

GC content: 52.82

Gene sequence:

>708_bases
ATGGCTGGGTTGGATAAACGGGTCGCTACGTATCACGAGGCGCTTGAAGGACTTACCAATGAGATGACGATTCTGGCAGG
AGGTTTTGGCCTGTGCGGCATCCCGGAGAATCTGATTGCTGAAGTTCGCCACCGTGGAGTAACCGGATTAACGGTGGTTT
CCAATAACTGTGGCGTAGATGGTTTTGGACTGGGTGTACTACTGGAAAGCCGACAGATCCGTAAAGTCGTCGCTTCTTAT
GTTGGCGAGAATGCTTTGTTTGAAAAACAGGTGCTTAACGGCGAACTGGAGGCGATTCTCACACCACAAGGAACCCTGGC
AGAACAACTGCGCGCAGGCGGCGCGGGTATCCCGGCGTTCTTTACTGCCACCGGTTACGGTACTCCCGTTGCCGAAGGGA
AAGAAGTCCGCGAATTCAACGGTCGCCCGTACATCATGGAACAGGCTATCACCGGTGATTTTGCCCTGGTCAAAGGCTGG
AAAGCCGACTGGTACGGCAACGTGATTTATCGTCATACCGCGCAAAACTTCAACCCACTGATGGCGGCTGCCGGAAAAAT
CACCGTGGTGGAAGTGGAAGAAATAGTGCCACCTGGTGAACTTGATCCTGCCGCTATCCATACCCCGGGGATTTTTGTTA
ATCGCGTCATTCAGGGGCAATTTGAAAAACGCATTGAACAACGCACCCTTAGCCGCAAAGGAGCCTGA

Upstream 100 bases:

>100_bases
ATATTGATAAGTAATACAAATGAATGAGTCACAAATTTAAAATTAACAAATCATTTTTTGCCCTTAATGATGTTGAAAAG
GACAACTGGAGGGCAACTGT

Downstream 100 bases:

>100_bases
CCATGTTAACCCGTGAACAAATGGCGATGCGAGTCGCCCGCGAATTGCGCGATGGCTATTACGTCAATCTCGGCATTGGT
ATCCCGACCCTGGTGGCAAA

Product: 3-oxoadipate CoA-transferase, subunit A

Products: NA

Alternate protein names: Succinyl CoA:3-oxoacid CoA-transferase; OXCT A [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MAGLDKRVATYHEALEGLTNEMTILAGGFGLCGIPENLIAEVRHRGVTGLTVVSNNCGVDGFGLGVLLESRQIRKVVASY
VGENALFEKQVLNGELEAILTPQGTLAEQLRAGGAGIPAFFTATGYGTPVAEGKEVREFNGRPYIMEQAITGDFALVKGW
KADWYGNVIYRHTAQNFNPLMAAAGKITVVEVEEIVPPGELDPAAIHTPGIFVNRVIQGQFEKRIEQRTLSRKGA

Sequences:

>Translated_235_residues
MAGLDKRVATYHEALEGLTNEMTILAGGFGLCGIPENLIAEVRHRGVTGLTVVSNNCGVDGFGLGVLLESRQIRKVVASY
VGENALFEKQVLNGELEAILTPQGTLAEQLRAGGAGIPAFFTATGYGTPVAEGKEVREFNGRPYIMEQAITGDFALVKGW
KADWYGNVIYRHTAQNFNPLMAAAGKITVVEVEEIVPPGELDPAAIHTPGIFVNRVIQGQFEKRIEQRTLSRKGA
>Mature_234_residues
AGLDKRVATYHEALEGLTNEMTILAGGFGLCGIPENLIAEVRHRGVTGLTVVSNNCGVDGFGLGVLLESRQIRKVVASYV
GENALFEKQVLNGELEAILTPQGTLAEQLRAGGAGIPAFFTATGYGTPVAEGKEVREFNGRPYIMEQAITGDFALVKGWK
ADWYGNVIYRHTAQNFNPLMAAAGKITVVEVEEIVPPGELDPAAIHTPGIFVNRVIQGQFEKRIEQRTLSRKGA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG1788

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit A family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=241, Percent_Identity=58.0912863070539, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI11545841, Length=240, Percent_Identity=55, Blast_Score=242, Evalue=2e-64,
Organism=Escherichia coli, GI1788551, Length=220, Percent_Identity=38.6363636363636, Blast_Score=173, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17531517, Length=246, Percent_Identity=50.4065040650407, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI21358325, Length=238, Percent_Identity=50.4201680672269, Blast_Score=234, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24655794, Length=238, Percent_Identity=50.4201680672269, Blast_Score=233, Evalue=6e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012792
- InterPro:   IPR004165
- InterPro:   IPR004163 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 25296; Mature: 25165

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLDKRVATYHEALEGLTNEMTILAGGFGLCGIPENLIAEVRHRGVTGLTVVSNNCGVD
CCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCC
GFGLGVLLESRQIRKVVASYVGENALFEKQVLNGELEAILTPQGTLAEQLRAGGAGIPAF
CCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCCCCE
FTATGYGTPVAEGKEVREFNGRPYIMEQAITGDFALVKGWKADWYGNVIYRHTAQNFNPL
EEECCCCCCCCCCCHHHHCCCCCEEEEHHCCCCCCEEECCCCCCCCCEEEEECCCCCCHH
MAAAGKITVVEVEEIVPPGELDPAAIHTPGIFVNRVIQGQFEKRIEQRTLSRKGA
HHCCCCEEEEEHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGLDKRVATYHEALEGLTNEMTILAGGFGLCGIPENLIAEVRHRGVTGLTVVSNNCGVD
CCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCC
GFGLGVLLESRQIRKVVASYVGENALFEKQVLNGELEAILTPQGTLAEQLRAGGAGIPAF
CCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCCCCE
FTATGYGTPVAEGKEVREFNGRPYIMEQAITGDFALVKGWKADWYGNVIYRHTAQNFNPL
EEECCCCCCCCCCCHHHHCCCCCEEEEHHCCCCCCEEECCCCCCCCCEEEEECCCCCCHH
MAAAGKITVVEVEEIVPPGELDPAAIHTPGIFVNRVIQGQFEKRIEQRTLSRKGA
HHCCCCEEEEEHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]