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The map label for this gene is ibpB

Identifier: 157157574

GI number: 157157574

Start: 4183231

End: 4183659

Strand: Reverse

Name: ibpB

Synonym: EcE24377A_4194

Alternate gene names: 157157574

Gene position: 4183659-4183231 (Counterclockwise)

Preceding gene: 157155984

Following gene: 157159179

Centisome position: 84.02

GC content: 48.95

Gene sequence:

>429_bases
ATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTGGCCAACGCACTGCAAAACGCCGG
TGAAAGCCAGAGCTTCCCGCCGTACAACATTGAGAAAAGCGACGATAACCACTACCGCATTACCCTTGCGCTGGCAGGTT
TCCGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAG
AAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAGCCTGAGCTTTACGCTGGCTGAAAATATGGAAGTCTCTGG
CGCAACCTTCGTAAACGGTTTACTGCATATTGATTTAATTCGTAATGAGCCTGAACCCATCGCAGCGCAGCGTATCGCTA
TCAGCGAACGTCCCGCGTTAAATAGCTAA

Upstream 100 bases:

>100_bases
CGCCTGGCGCGGCCTGACATCTCCATGCTCGCCGTCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCT
TCTTAGAAGGAGAAATGACT

Downstream 100 bases:

>100_bases
CTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTTCTTCTTCAACAATGCGTTAAATTTCGCCTGT
TTAAGATATTTCGGCACGTT

Product: heat shock chaperone IbpB

Products: NA

Alternate protein names: 16 kDa heat shock protein B

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNHYRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEE
KKWLHQGLMNQPFSLSFTLAENMEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS

Sequences:

>Translated_142_residues
MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNHYRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEE
KKWLHQGLMNQPFSLSFTLAENMEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS
>Mature_142_residues
MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNHYRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEE
KKWLHQGLMNQPFSLSFTLAENMEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS

Specific function: Associates with aggregated proteins, together with IbpA, to stabilize and protect them from irreversible denaturation and extensive proteolysis during heat shock and oxidative stress. Aggregated proteins bound to the IbpAB complex are more efficiently ref

COG id: COG0071

COG function: function code O; Molecular chaperone (small heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small heat shock protein (HSP20) family

Homologues:

Organism=Escherichia coli, GI87082316, Length=142, Percent_Identity=100, Blast_Score=293, Evalue=4e-81,
Organism=Escherichia coli, GI1790122, Length=138, Percent_Identity=50.7246376811594, Blast_Score=134, Evalue=2e-33,

Paralogues:

None

Copy number: 686 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): IBPB_ECO24 (A7ZTP0)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001465169.1
- ProteinModelPortal:   A7ZTP0
- SMR:   A7ZTP0
- STRING:   A7ZTP0
- EnsemblBacteria:   EBESCT00000019205
- GeneID:   5586090
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_4194
- eggNOG:   COG0071
- GeneTree:   EBGT00050000010332
- HOGENOM:   HBG748242
- OMA:   NQLLIRG
- ProtClustDB:   PRK11597
- BioCyc:   ECOL331111:ECE24377A_4194-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02001
- InterPro:   IPR002068
- InterPro:   IPR008978
- InterPro:   IPR022848

Pfam domain/function: PF00011 HSP20; SSF49764 HSP20_chap

EC number: NA

Molecular weight: Translated: 16093; Mature: 16093

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS01031 HSP20

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNHYRITLALAGFRQEDLE
CCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCE
IQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAENMEVSGATFVNGLLHIDLI
EEEECCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHEEEEEEE
RNEPEPIAAQRIAISERPALNS
CCCCCCCHHHEEECCCCCCCCC
>Mature Secondary Structure
MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNHYRITLALAGFRQEDLE
CCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCE
IQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAENMEVSGATFVNGLLHIDLI
EEEECCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHEEEEEEE
RNEPEPIAAQRIAISERPALNS
CCCCCCCHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA