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The map label for this gene is ysaA [H]

Identifier: 157157371

GI number: 157157371

Start: 4053730

End: 4054203

Strand: Reverse

Name: ysaA [H]

Synonym: EcE24377A_4070

Alternate gene names: 157157371

Gene position: 4054203-4053730 (Counterclockwise)

Preceding gene: 157156720

Following gene: 157155273

Centisome position: 81.42

GC content: 53.8

Gene sequence:

>474_bases
ATGAACCGGTTTATTATTGCGGATGCGACGAAATGTATCGGTTGCCGTACCTGTGAAGTGGCTTGCGCAGTGTCGCATCA
GGATAATCAGGATTGCGCTGCGTTGTCACCAGACGAGTTTATTTCCCGTATTCGTGTCATTAAAGACCACAGCTGGACCA
CGGCAGTAGCCTGTCATCAGTGTGAAGATGCACCGTGCGCGAATGTCTGCCCTGTTGACGCGATAAGCCGCGAACATGGG
CATATTTTCGTTGAACAAACACGTTGCATTGGCTGTAAAAGCTGTATGCTGGCTTGCCCGTTTGGTGCGATGGAGGTCGT
TTCTTCGCGCAAAAAGGCGAGGGCGATTAAGTGCGATCTGTGCTGGCATCGGGAGACGGGGCCGGCCTGTGTTGAAGCCT
GCCCGACAAAGGCGTTGCAGTGCATGGATGTCGAGAAAGTGCAGCGGCATCGGCTACGGCAGCAGCCTGTTTGA

Upstream 100 bases:

>100_bases
GCTTTGGACAAGTGCCAAAACTTTAACATTTCCTTCGTTGGATCAAAGCAGTAGGGACGCGCTCTCTGGCACTCTGCTGT
TTTAGTGCAAAGGAGTGATC

Downstream 100 bases:

>100_bases
AACGTTTTCCGGTGTTTCTCAACGCCGGAATAGTGCGCTTTGTTTATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTA
CAAATTCTTCCAAATTCAAT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MNRFIIADATKCIGCRTCEVACAVSHQDNQDCAALSPDEFISRIRVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHG
HIFVEQTRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV

Sequences:

>Translated_157_residues
MNRFIIADATKCIGCRTCEVACAVSHQDNQDCAALSPDEFISRIRVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHG
HIFVEQTRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV
>Mature_157_residues
MNRFIIADATKCIGCRTCEVACAVSHQDNQDCAALSPDEFISRIRVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHG
HIFVEQTRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367245, Length=157, Percent_Identity=98.0891719745223, Blast_Score=313, Evalue=4e-87,
Organism=Escherichia coli, GI1789067, Length=162, Percent_Identity=61.7283950617284, Blast_Score=197, Evalue=3e-52,
Organism=Escherichia coli, GI1788811, Length=147, Percent_Identity=53.0612244897959, Blast_Score=148, Evalue=1e-37,
Organism=Escherichia coli, GI87082179, Length=153, Percent_Identity=46.4052287581699, Blast_Score=140, Evalue=3e-35,
Organism=Escherichia coli, GI87082180, Length=156, Percent_Identity=44.8717948717949, Blast_Score=135, Evalue=1e-33,
Organism=Escherichia coli, GI1789079, Length=187, Percent_Identity=39.0374331550802, Blast_Score=127, Evalue=2e-31,
Organism=Escherichia coli, GI87082114, Length=190, Percent_Identity=33.6842105263158, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787872, Length=165, Percent_Identity=32.7272727272727, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1787122, Length=163, Percent_Identity=31.9018404907975, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1787963, Length=161, Percent_Identity=31.055900621118, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI226510944, Length=144, Percent_Identity=36.1111111111111, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI2367345, Length=173, Percent_Identity=31.7919075144509, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17420; Mature: 17420

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
14.0 %Cys+Met (Translated Protein)
11.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
14.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIIADATKCIGCRTCEVACAVSHQDNQDCAALSPDEFISRIRVIKDHSWTTAVACHQ
CCEEEEECCCHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHC
CEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDL
CCCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHCEEEEEE
CWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV
EECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRFIIADATKCIGCRTCEVACAVSHQDNQDCAALSPDEFISRIRVIKDHSWTTAVACHQ
CCEEEEECCCHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHC
CEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDL
CCCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHCEEEEEE
CWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV
EECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]