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The map label for this gene is pmrD [H]

Identifier: 157157275

GI number: 157157275

Start: 2548129

End: 2548395

Strand: Reverse

Name: pmrD [H]

Synonym: EcE24377A_2554

Alternate gene names: 157157275

Gene position: 2548395-2548129 (Counterclockwise)

Preceding gene: 157158327

Following gene: 157159312

Centisome position: 51.18

GC content: 47.57

Gene sequence:

>267_bases
ATGGAATGGCTGGTCAAAAAATCGTGCTGCAATAAACAAGATAACAGACATGTACTTATGCTCTGCGATGCTGGCGGCGC
GATAAAAATGATCGCCGAAGTGAAGAGTGACTTCGCCGTGAAAGTGGGGGATTTACTCTCGCCTTTGCAGAATGCGCTTT
ATTGTATTAATCGTGAAAAGCTGCATACGGTGAAAGTCCTCTCGGCAAGCAGTTATAGCCCCGATGAGTGGGAACGGCAG
TGTACAGCGGCAGGGAAAACTCAGTAA

Upstream 100 bases:

>100_bases
CGTGTCACTTCTCATTTCTGCCATTCCATTGCCGTTGATAAAGAGTTTATCTGTTAATCTGTAAATTGATGTGAAAACTC
TTAGCAAACAGGATAATGCA

Downstream 100 bases:

>100_bases
CGTTGTTTTGTCGTGGGCAGGAAAATCAGCATCAACCCGCTCATAATACAGGCTACTCCCAGTAGTGCTTTCAGCGAAAA
AGTCCCTTCCCAGCCGGGAA

Product: polymyxin B resistance protein pmrD

Products: NA

Alternate protein names: BasR post-transcriptional activator; Polymyxin resistance protein pmrD [H]

Number of amino acids: Translated: 88; Mature: 88

Protein sequence:

>88_residues
MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQ
CTAAGKTQ

Sequences:

>Translated_88_residues
MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQ
CTAAGKTQ
>Mature_88_residues
MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQ
CTAAGKTQ

Specific function: Interacts with phosphorylated BasR protein to mediate transcriptional induction of BasR-activated genes to induce polymyxin resistance in some natural isolates [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pmrD family [H]

Homologues:

Organism=Escherichia coli, GI87082076, Length=88, Percent_Identity=97.7272727272727, Blast_Score=180, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020146
- ProDom:   PD028730 [H]

Pfam domain/function: PF11183 PmrD [H]

EC number: NA

Molecular weight: Translated: 9815; Mature: 9815

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
5.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
9.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGDLLSPLQNALYCINREK
CCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
LHTVKVLSASSYSPDEWERQCTAAGKTQ
EEEEEEEECCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGDLLSPLQNALYCINREK
CCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
LHTVKVLSASSYSPDEWERQCTAAGKTQ
EEEEEEEECCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626063; 9205837; 9278503 [H]