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The map label for this gene is gadW [H]

Identifier: 157157215

GI number: 157157215

Start: 3975701

End: 3976429

Strand: Reverse

Name: gadW [H]

Synonym: EcE24377A_4001

Alternate gene names: 157157215

Gene position: 3976429-3975701 (Counterclockwise)

Preceding gene: 157155338

Following gene: 157158855

Centisome position: 79.85

GC content: 41.7

Gene sequence:

>729_bases
ATGACTCATGTCTGCTCGGTGATCCTCATTCGTCGTTCATTCGATATTTATCATGAACAGCATAAAATATCGCTGCATAA
CGAGAGTATCGTGCTGCTGGAGAAAAATTTGGCAGACGATTTTGCGTTTTGTTCACCGGATACGCGACGACTGGATATCG
ATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACATAGCAAAGACCGTCTA
TTAATTAACCAGTTACCCCCCATGCCGCTGGTGACGGCGATTTTTGATAGCTTCAATGAATCCGGGGTAAATTCACCGAT
ACTGAGCAATATGCTCTACCTTTCCTGTTTATCGATGTTTTCTCATAAGAAAGAACTGATCCCCTTACTTTTCAATAGCA
TCAGCACTGTTTCAGGAAAAGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGAA
AGAATGTATACCAGCGAGAGTCTCATCAAAAAAAAGTTGCAGGATGAAAATACCTGTTTCAGTAAAATATTACTCGCTTC
CAGGATGTCGATGGCCAGACGATTACTCGAGTTACGTCAAATTCCTCTGCATACTATTGCAGAAAAATGTGGCTATAGCA
GTACGTCGTACTTTATAAACACATTTCGACAATATTATGGTGTAACGCCACATCAGTTTGCGCAACATTCGCCAGGTACC
TTTTCCTGA

Upstream 100 bases:

>100_bases
CTACCAAATCTGGCAGTTTTTTCGCTAAGAAACAGTCTGGCAACATTTCATTAGTATACTGAAATTGAAATAATCGCAGT
ATGAAATATAAGGGATAATC

Downstream 100 bases:

>100_bases
CATATTTTGCATTTGAATATTGGTCAGGATCTCACACCTGCTTCATGTGAAACTCTTCCCTGATGATTTCTGCCGGGCTA
CCGGCTAGTTCTCTTTCGCA

Product: transcriptional regulator GadW

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFCSPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRL
LINQLPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
FS

Sequences:

>Translated_242_residues
MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFCSPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRL
LINQLPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
FS
>Mature_241_residues
THVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFCSPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLL
INQLPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAER
MYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGTF
S

Specific function: Depending on the conditions (growth phase and medium), acts as a positive or negative regulator of gadA and gadBC. Repression occurs directly or via the repression of the expression of gadX. Activation occurs directly by the binding of gadW to the gadA an

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789932, Length=242, Percent_Identity=98.7603305785124, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1786776, Length=241, Percent_Identity=35.6846473029046, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI1787776, Length=237, Percent_Identity=33.7552742616034, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789933, Length=132, Percent_Identity=37.8787878787879, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1790557, Length=133, Percent_Identity=37.593984962406, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1786778, Length=124, Percent_Identity=41.1290322580645, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 28050; Mature: 27919

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFCSPDTRRLDIDELTVCH
CCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHH
YLQNIRQLPRNLGLHSKDRLLINQLPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMF
HHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAERMYTSESLIKKKLQDENTCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCCC
FS
CC
>Mature Secondary Structure 
THVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFCSPDTRRLDIDELTVCH
CHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHH
YLQNIRQLPRNLGLHSKDRLLINQLPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMF
HHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAERMYTSESLIKKKLQDENTCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCCC
FS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]