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The map label for this gene is rlmG [H]

Identifier: 157157132

GI number: 157157132

Start: 3563970

End: 3565106

Strand: Reverse

Name: rlmG [H]

Synonym: EcE24377A_3551

Alternate gene names: 157157132

Gene position: 3565106-3563970 (Counterclockwise)

Preceding gene: 157156983

Following gene: 157155932

Centisome position: 71.59

GC content: 54.0

Gene sequence:

>1137_bases
ATGAGCCACTTAGACAACGGTTTCCGTTCACTGACACTACAACGTTTTCCGGCGACGGATGACGTTAACCCGCTACAGGC
GTGGGAAGCGGCGGATGAATATTTGCTGCAACAGTTGGACGACACAGAAATCCGCGGCCCGGTGTTGATCCTGAATGATG
CCTTTGGTGCGTTAAGCTGCGCGCTGGCGGAACATAAGCCGTACAGCATTGGCGACTCATACATCAGTGAACTGGCGACG
CGCGAGAATTTACGCCTCAACGGGATTGATGAGTCGAGCGTGAAGTTTCTCGACAGCACCGCCGACTACCCGCAACAGCC
GGGTGTGGTGCTGATCAAAGTGCCGAAAACACTGGCATTGCTGGAACAGCAACTGCGTGCGCTGCGCAAAGTGGTCACGT
CGGATACACGTATTATTGCCGGTGCCAAAGCCCGTGACATTCACACTTCCACGCTGGAGCTGTTCGAGAAAGTGCTCGGC
CCGACCACCACTACGCTGGCATGGAAAAAAGCGCGCCTGATTAATTGCACTTTCAATGAACCGCCGCTGGCCGATGCGCC
GCAGACCGTTAGCTGGAAGCTGGAAGGTACTGACTGGACTATCCACAACCATGCGAATGTCTTCTCCCGCACCGGGCTGG
ATATTGGCGCACGCTTCTTTATGCAACATCTGCCAGAGAATCTCGAAGGTGAGATTGTCGATCTCGGCTGTGGTAACGGC
GTTATTGGCCTGACGCTGCTTGATAAAAACCCGCAGGCGAAAGTGGTGTTTGTCGATGAATCGCCGATGGCGGTCGCTTC
CAGCCGAATGAATGTTGAAACCAACATGCCAGAGGCGTTGGATCGCTGCGAGTTTATGATCAACAACGCGCTCTCCGGAG
TGAAGCCTTTCCGCTTTAATGCTGTGCTCTGCAACCCGCCGTTTCATCAGCAACATGCGCTGACCGATAACGTCGCCTGG
GAGATGTTCCACCACGCCCGCCGCTGCCTGAAAATCAACGGCGAACTGTATATCGTTGCCAACCGTCACCTGGATTACTT
CCATAAACTGAAGAAGATTTTCGGCAACTGCACCACCATCGCCACGAATAATAAATTTGTGGTGCTGAAAACAGTGAAGC
TGGGGCGTCGTCGGTAA

Upstream 100 bases:

>100_bases
AAGTAAGATTGCTCATAACCGCCCACGAAAAAGAGAAAAAGGGTATACTCACGCACCCTTTTCAGGGGCAACGTCGAAAT
TTTATCATTCAGGAGGGCCG

Downstream 100 bases:

>100_bases
GATTGGGAGTTACCTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACGTTCAGCACGTTACTCCCAGGCCGGATAAGA
CGCGGCAAGCGTCGCATCCG

Product: methyltransferase family protein

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEIRGPVLILNDAFGALSCALAEHKPYSIGDSYISELAT
RENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
VIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNMPEALDRCEFMINNALSGVKPFRFNAVLCNPPFHQQHALTDNVAW
EMFHHARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR

Sequences:

>Translated_378_residues
MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEIRGPVLILNDAFGALSCALAEHKPYSIGDSYISELAT
RENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
VIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNMPEALDRCEFMINNALSGVKPFRFNAVLCNPPFHQQHALTDNVAW
EMFHHARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR
>Mature_377_residues
SHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEIRGPVLILNDAFGALSCALAEHKPYSIGDSYISELATR
ENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLGP
TTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNGV
IGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNMPEALDRCEFMINNALSGVKPFRFNAVLCNPPFHQQHALTDNVAWE
MFHHARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA [H]

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family [H]

Homologues:

Organism=Escherichia coli, GI87082206, Length=378, Percent_Identity=98.9417989417989, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI1790830, Length=173, Percent_Identity=33.5260115606936, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR017237
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.174 [H]

Molecular weight: Translated: 42348; Mature: 42217

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEIRGPVLILNDAFGALSC
CCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHH
ALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLAL
HHHCCCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCCCCCCEEEEECCHHHHH
LEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLGPTTTTLAWKKARLINCTFNE
HHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCC
PPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
CCCCCCCCCEEEEECCCEEEEECCCCHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCC
VIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNMPEALDRCEFMINNALSGVKPFRFN
EEEEEEECCCCCEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
AVLCNPPFHQQHALTDNVAWEMFHHARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTI
EEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCEEEEEECCCHHHHHHHHHHHCCCEEE
ATNNKFVVLKTVKLGRRR
EECCCEEEEEECCCCCCC
>Mature Secondary Structure 
SHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEIRGPVLILNDAFGALSC
CCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHH
ALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLAL
HHHCCCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCCCCCCEEEEECCHHHHH
LEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLGPTTTTLAWKKARLINCTFNE
HHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCC
PPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
CCCCCCCCCEEEEECCCEEEEECCCCHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCC
VIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNMPEALDRCEFMINNALSGVKPFRFN
EEEEEEECCCCCEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
AVLCNPPFHQQHALTDNVAWEMFHHARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTI
EEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCEEEEEECCCHHHHHHHHHHHCCCEEE
ATNNKFVVLKTVKLGRRR
EECCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA